Comparative Analysis of Chemical Constituents by HPLC‐ESI‐MS n and Antioxidant Activities of Dendrobium huoshanense and Dendrobium officinale

2021 ◽  
Author(s):  
Jingqiong Wan ◽  
Xiaohui Gong ◽  
Feixuan Wang ◽  
Chongwei Wen ◽  
Yuan Wei ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Dendrobium Officinale ◽  
Esi Ms ◽  
Dendrobium Huoshanense

scholarly journals First Systematic Analysis for Chemical Composition by HPLC-ESI-MSn and Antioxidant Activity Comparison of Dendrobium huoshanense and Dendrobium officinale

2020 ◽  
Author(s):  
Jingqiong Wan ◽  
Xiaohui Gong ◽  
Chongwei Wen ◽  
Yuan Wei ◽  
Lingdong Kong ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antioxidant Activities ◽  
Scientific Basis ◽  
Dendrobium Officinale ◽  
Oh Radicals ◽  
High Quality ◽  
Systematic Analysis ◽  
Dendrobium Huoshanense

Abstract Background: Dendrobium huoshanense C. Z. Tang et S. J. Cheng (D. huoshanense) is a precious Dao-di herb with high quality, excellent efficacy and edible value of China, Dendrobium officinale Kimura et Migo (D. officinale) is widely utilized and commonly used as a substitute for D. huoshanense. But few studies have been conducted to systematically analyze chemical composition and bioactivities of the two.Methods: HPLC-ESI-MSn was used to identify the compounds in D. huoshanense and D. officinale grown in Huoshan from methanol extracts and different polar fractions of water extracts, including petroleum ether, ethyl acetate and n-butanol; The in vitro antioxidant activity were evaluated by the scavenging effects on DPPH and ·OH radicals, total reduction ability and total antioxidant ability.Results: 61 compounds were identified in D. huoshanense and 49 compounds were identified in D. officinale and with 43 shared compounds; Among them, 44 and 7 compounds were respectively identified from the two for the first time. Oxyresveratrol, quercetin-3-O-glucoside, quercetin-3-O-arabinoside, naringenin-7-O-glucoside, baicalin, hesperetin, vitexin and apigenin-6-C-pentosyl-8-C-(2’’-O-quinoyl)-glucoside were first identified in Dendrobium. In addition, 5 compounds were unique in D. huoshanense compared with other species of Dendrobium . The results of in vitro antioxidant activity showed that n-BuOH-soluble fraction of D. huoshanense and D. officinale both revealed the strongest antioxidant activities compared with other fractions, furthermore, the antioxidant activities of different fractions of D. huoshanense were separately superior to that of D. officinale.Conclusion: These results may provide a reference for the substances study about D. huoshanense's high-quality efficacy, and also provide a scientific basis for its development and utilization.

Investigation of Compounds and Biological Activity of Selected Indonesian Marine Sponges

2020 ◽  
Vol 10 (3) ◽  
pp. 312-321
Author(s):  
Idin Sahidin ◽  
Carla W. Sabandar ◽  
Wahyuni ◽  
Rini Hamsidi ◽  
Sandra Aulia Mardikasari ◽  
...  
Keyword(s):  
Biological Activity ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Marine Sponges ◽  
Chemical Screening ◽  
Methanol Extracts ◽  
Ms Analysis ◽  
Antioxidant Agents

Background: Marine sponges provided a great source of natural products with promising biological activity. This study was aimed to investigate the chemical constituents of methanol extracts of selected Indonesian marine sponges (Callyspongia sp., Clathria sp., Melophlus sarasinorum, and Xestospongia sp.), collected from the Saponda Islands, Southeast Sulawesi, Indonesia as well as to evaluate their antimicrobial and antioxidant activities. Methods: LCMS/MS analysis used to identify the compounds. Agar well diffusion and DPPH assays were used to evaluate the antimicrobial and antioxidant activities. Results: Chemical screening reported alkaloids, terpenoids, steroids, and saponins from all investigated sponges. The LC-MS/MS analysis identified various compounds which mainly contained steroids. Antimicrobial activity (against Bacillus subtilis, Escherichia coli, Salmonella enterica, and Candida albicans) was only shown by the Xestospongia sp. extract. Meanwhile, extracts of M. sarasinorum, Xestospongia sp., and Callyspongia sp. exhibited potent radical scavenging activity. Conclusion: The study concluded that the selected sponges could provide various groups of compounds. Methanol extracts of these sponges could be used as sources of antimicrobial and antioxidant agents.

Chemical Constituents Isolated from Rhododendron ungernii with Antioxidant Profile

2019 ◽  
Vol 9 (3) ◽  
pp. 238-243 ◽  
Author(s):  
Emine Dede ◽  
Nusret Genc ◽  
Mahfuz Elmastas ◽  
Huseyin Aksit ◽  
Ramazan Erenler
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Isolation And Identification ◽  
Reduced Pressure ◽  
Scavenging Effect ◽  
Pharmaceutical Industries ◽  
Dpph Scavenging

Background: Plant in Rhododendron genus that contains phenolic compounds has been used in traditional medicine and revealed considerable biological activities. Objective: Isolation and identification of antioxidant natural products from Rhododendron ungernii. Methods: Rhododendron ungernii Trautv. flowers were collected and dried in shade. The dried flowers were extracted with methanol for 3 days. The solvent was removed by reduced pressure to yield the extract which was subjected to column chromatography (Sephadex LH-20, C18 reversed phase column) to isolate catechin-7-O-glucoside (1), quercetin-3-O-β-galactoside (2), quercetin-3-O- β-xyloside (3), farrerol (4), myricetin (5), and quercetin (6). The structures of isolated compounds were elucidated by spectroscopic methods such as 1D-NMR, 2D-NMR, and LC-TOF/MS. DPPH scavenging effect, ABTS+ scavenging activity, and reducing power (FRAP) were performed for antioxidant assays of isolated natural compounds. Results: Isolated flavonoids displayed the outstanding antioxidant activities. Catechin-7-O-glucoside (1) and quercetin-3-O-β-galactoside (2) (IC50, 3.66 µg/mL) had the most DPPH• scavenging effect among the compounds. The highest ABTS•+ scavenging activity (IC50, 1.41 µg/mL) and reducing power effect (6.05 mmol TE/g comp) were observed for myricetin (5). Conclusion: R. ungernii extract and isolated compounds could be a promising antioxidant for food and pharmaceutical industries.

scholarly journals Determination of Chemical Constituents and Antioxidant Activities of Leaves and Stems from Jatropha cinerea (Ortega) Müll. Arg and Jatropha cordata (Ortega) Müll. Arg

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 212
Author(s):  
Yeimi Cecilia Vega-Ruiz ◽  
Corina Hayano-Kanashiro ◽  
Nohemí Gámez-Meza ◽  
Luis Angel Medina-Juárez
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biological Effects ◽  
Chemical Constituents ◽  
Weather Conditions ◽  
Antioxidant Compounds ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Northwestern Region ◽  
High Concentrations

Jatropha species have been shown to be an important source of secondary metabolites with different biological effects. Jatropha cinerea (Ortega) Müll. Arg and Jatropha cordata (Ortega) Müll. Arg are distributed in the Northwestern region of Mexico, are adapted to extreme weather conditions and are widely used (stems, leaves, and sap) in traditional medicine. The aim of the present study was to carry out the phytochemical characterization and the evaluation of the antioxidant activity in methanolic extracts of stems and leaves from J. cinerea and J. cordata. The compounds present in the extracts of both species were characterized by ESI-IT-MS/MS and quantified by HPLC-DAD. The results showed that the stem extracts of both species are rich in phenolic acids, while the leaf extracts are rich in flavonoids. Some of the main compounds found were gallic acid, gentisic acid, 3,4-Dihydroxybenzoic acid, vitexin, isovitexin, and catechol. Both species showed high concentrations of phenols and total flavonoids and antioxidant activity. J. cordata showed the highest antioxidant capacity and the highest concentration of phenolic compounds. Overall, both Jatropha species are a natural source of antioxidant compounds with potential biotechnological uses.

scholarly journals The Dual Antimelanogenic and Antioxidant Activities of the Essential Oil Extracted from the Leaves ofAcorus macrospadiceus(Yamamoto) F. N. Wei et Y. K. Li

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Huey-Chun Huang ◽  
Hsiao-Fen Wang ◽  
Kuang-Hway Yih ◽  
Long-Zen Chang ◽  
Tsong-Min Chang
Keyword(s):  
Essential Oil ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Reducing Power ◽  
Cellular Level ◽  
Tyrosinase Activity ◽  
Oxygen Species ◽  
Melanin Content ◽  
Chelating Activity

The antimelanogenic and antioxidant activities of the essential oil extracted from the leaves ofAcorus macrospadiceus(Yamamoto) F. N. Wei et Y. K. Li have never been explored. The essential oil effectively inhibited mushroom tyrosinase activity (EC50= 1.57 mg/mL) and B16F10 tyrosinase activity (IC50= 1.01 mg/mL), decreased the melanin content (EC50= 1.04 mg/mL), and depleted the cellular level of the reactive oxygen species (ROS) (EC50= 1.87 mg/mL). The essential oil effectively scavenged 2,2-diphenyl-1-picryl-hydrazyl (DPPH) (EC50= 0.121 mg/mL) and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) ABTS+radicals (EC50= 0.122 mg/mL). It also exhibited an apparent reducing power (EC50= 0.021 mg/mL) and metal-ion chelating activity (EC50= 0.029 mg/mL). The chemical constituents of the essential oil are ethers (55.73%), ketones (19.57%), monoterpenes (7.82%), alcohols (3.85%), esters (3.77%), sesquiterpenes (3.72%), and aromatic compounds (2.85%). The results confirm thatA. macrospadiceusessential oil is a natural antioxidant and inhibitor of melanogenesis.

scholarly journals Extraction, Radical Scavenging and Antimicrobial Activity of Essential Oils of Root of Dryopteris marginalis

2019 ◽  
Vol 31 (11) ◽  
pp. 2512-2516 ◽  
Author(s):  
Md. Zahidul Islam ◽  
Jannat Al Foisal ◽  
Mahbubur Rahman ◽  
Mst. Afsana Mimi ◽  
Faridul Islam ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Total Concentration ◽  
Petroleum Ether Extract ◽  
Radical Scavenging ◽  
Organic Extracts ◽  
Zones Of Inhibition ◽  
Essential Oil Yield

The aim of the study was to characterize the chemical constituents and determine antibacterial and antioxidant activities of essential oils and three different extracts of Dryopteris marginalis. The root had essential oil yield of 0.36 % (w/w) in which 12 organic compounds representing 97.22% of the root oils were identified. Tyranton was found as the most abundant component with 77.571 % of the total concentration in the essential oil. The zones of inhibition of different organic extracts against the tested bacteria were found in the range of 6.5-15 mm. Pseudomonas was the most vulnerable with MICs of 15.62 μg/mL by both ethyl acetate and petroleum ether extract producing 3.8 mm zone of inhibition. The essential oils extracted from roots of Dryopteris marginalis showed maximum 85.29 % inhibition of radical scavenging at 2 mg/mL concentration. Among all root extracts, methanol extract exhibited 41.11 % inhibition at 2 mg/mL concentration.

Anti-osteoporotic and antioxidant activities of chemical constituents of the aerial parts of Ducrosia ismaelis

2014 ◽  
Vol 24 (15) ◽  
pp. 3434-3439 ◽  
Author(s):  
Abubaker M.A. Morgan ◽  
Hyun Woo Lee ◽  
Sang-Hyun Lee ◽  
Chi-Hwan Lim ◽  
Hae-Dong Jang ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Aerial Parts

scholarly journals Accurate authentication of Dendrobium officinale and its closely related species by comparative analysis of complete plastomes

2018 ◽  
Vol 8 (6) ◽  
pp. 969-980 ◽  
Author(s):  
Shuying Zhu ◽  
Zhitao Niu ◽  
Qingyun Xue ◽  
Hui Wang ◽  
Xuezhu Xie ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Related Species ◽  
Dendrobium Officinale ◽  
Closely Related Species

scholarly journals Chemical Constituents from Walsura pinnata (Meliaceae)

2017 ◽  
Vol 12 (9) ◽  
pp. 1934578X1701200
Author(s):  
Mohamad A. Mahdzir ◽  
Jamil A. Shilpi ◽  
Norfaizah Mahmud ◽  
Sujatha Ramasamy ◽  
Khalijah Awang
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Chemical Constituents ◽  
Cancer Cell Lines ◽  
Phytochemical Study ◽  
Triterpene Acid ◽  
Human Cancer Cell Lines ◽  
Esi Ms

A phytochemical study on the bark of Walsura pinnata has led to the isolation of a new oleanane triterpene acid, 3-oxo-olean-9(11),12-dien-28-oic acid (1), together with nine known compounds (2–10). Their structures were established on the basis of the detailed spectroscopic analysis, including one- and two-dimensional NMR, ESI-MS and HR-ESI-MS techniques. Compounds 2, 3, 5, 6 and 8 were isolated from W. pinnata for the first time. Compounds 3 and 4 showed in vitro growth inhibitory activity against two human cancer cell lines MCF-7 and SK-OV-3 with IC50 values within the range of 8.85 - 18.28 μg/mL. To the best of our knowledge, this is the first report on the cytotoxic activity of compound 3 towards both cancer cell lines.

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