Investigation of the incompatibility of Knoxiae Radix and Glycyrrhizae Radix et Rhizoma in rats by 1 H NMR and MS‐based untargeted metabolomic analysis

2021 ◽  
Author(s):  
Xu Yan ◽  
Min Zhao ◽  
Wanru Zou ◽  
Pengyao Tian ◽  
Lin Sun ◽  
...  
Keyword(s):  
Metabolomic Analysis ◽  
Glycyrrhizae Radix ◽  
H Nmr

scholarly journals Putative imbalanced amino acid metabolism in rainbow trout long term fed a plant-based diet as revealed by 1H-NMR metabolomics

2021 ◽  
Vol 10 ◽  
Author(s):  
Catherine Deborde ◽  
Blandine Madji Hounoum ◽  
Annick Moing ◽  
Mickaël Maucourt ◽  
Daniel Jacob ◽  
...  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Proton Nuclear Magnetic Resonance ◽  
Free Form ◽  
Metabolomic Analysis ◽  
Nmr Metabolomics ◽  
H Nmr

Abstract The long-term effect of a plant (P)-based diet was assessed by proton nuclear magnetic resonance (1H-NMR) metabolomics in rainbow trout fed a marine fish meal (FM)–fish oil (FO) diet (M), a P-based diet and a control commercial-like diet (C) starting with the first feeding. Growth performances were not heavily altered by long-term feeding on the P-based diet. An 1H-NMR metabolomic analysis of the feed revealed significantly different soluble chemical compound profiles between the diets. A set of soluble chemical compounds was found to be specific either to the P-based diet or to the M diet. Pterin, a biomarker of plant feedstuffs, was identified both in the P-based diet and in the plasma of fish fed the P-based diet. 1H-NMR metabolomic analysis on fish plasma and liver and muscle tissues at 6 and 48 h post feeding revealed significantly different profiles between the P-based diet and the M diet, while the C diet showed intermediate results. A higher amino acid content was found in the plasma of fish fed the P-based diet compared with the M diet after 48 h, suggesting either a delayed delivery of the amino acids or a lower amino acid utilisation in the P-based diet. This was associated with an accumulation of essential amino acids and the depletion of glutamine in the muscle, together with an accumulation of choline in the liver. Combined with an anticipated absorption of methionine and lysine supplemented in free form, the present results suggest an imbalanced essential amino acid supply for protein metabolism in the muscle and for specific functions of the liver.

Metabolomic analysis of serum by (1) H NMR spectroscopy in amyotrophic lateral sclerosis

2010 ◽  
Vol 411 (17-18) ◽  
pp. 1383 ◽  
Author(s):  
G.N. Babu ◽  
Alok Kumar ◽  
Lakshmi Bala ◽  
Jayantee Kalita ◽  
U.K. Misra ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Nmr Spectroscopy ◽  
Metabolomic Analysis ◽  
H Nmr ◽  
Lateral Sclerosis

scholarly journals Diagnosis of papillary thyroid carcinoma by 1H NMR spectroscopy-based metabolomic analysis of whole blood

2020 ◽  
Vol 14 (4) ◽  
pp. 187-196
Author(s):  
Tiantian Wang ◽  
Zhigang Sun ◽  
Yong Wang ◽  
Feifei Li ◽  
Xiaoming Zhou ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Whole Blood ◽  
Metabolomic Analysis ◽  
Papillary Thyroid ◽  
H Nmr

scholarly journals Effect of rootstock on the scion of Hevea brasiliensis through metabolic analysis of latex samples by ¹H NMR

2011 ◽  
Vol 11 (spe) ◽  
pp. 82-88 ◽  
Author(s):  
Eduardo Sanches Pereira do Nascimento ◽  
Clayton Rodrigues de Oliveira ◽  
Paulo de Souza Gonçalves ◽  
Reginaldo Brito da Costa ◽  
Rogério Manoel Biagi Moreno ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Hevea Brasiliensis ◽  
Multivariate Data Analysis ◽  
Metabolic Profile ◽  
Metabolomic Analysis ◽  
Pca Analysis ◽  
H Nmr ◽  
Metabolic Analysis ◽  
Different Types ◽  
Grafting Through

In this study, the effect of rootstock on grafting through metabolomic analysis of latex (Hevea brasiliensis) samples was verified by ¹H nuclear magnetic resonance (NMR) and multivariate data analysis. Sixteen metabolites present in the latex cytosol were characterized by NMR. PCA analysis showed that the latex samples of the RR and GR groups can be differentiated. The GR group samples present a metabolic profile similar to the RR group samples, while the RG group is in an intermediate position between RR and GG groups. Sucrose and formate contributed greatly to the separation obtained by PCA, presenting a good correlation between the results. ¹H NMR was an efficient technique to differentiate latex samples from different types of rootstocks and grafting and in the future could be used to predict rubber production by latex analysis.

scholarly journals Metabolomic Analysis of Complex Chinese Remedies: Examples of Induced Nephrotoxicity in the Mouse from a Series of Remedies Containing Aristolochic Acid

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Dong-Ming Tsai ◽  
Jaw-Jou Kang ◽  
Shoei-Sheng Lee ◽  
San-Yuan Wang ◽  
I-Lin Tsai ◽  
...  
Keyword(s):  
Discriminant Analysis ◽  
Chinese Medicine ◽  
Aristolochic Acid ◽  
Renal Tubules ◽  
Herbal Formula ◽  
Metabolomic Analysis ◽  
Main Ingredient ◽  
H Nmr ◽  
Renal Tubular ◽  
Metabolomics Analysis

Aristolochic acid nephropathy is caused by aristolochic acid (AA) and AA-containing herbs. In traditional Chinese medicine, a principle called “Jun-Chen-Zou-Shi” may be utilized to construct a remedial herbal formula that attempts to mitigate the toxicity of the main ingredient. This study usedBu-Fei-A-Jiao-Tang(BFAJT) to test if the compound remedy based on a principle of “Jun-Chen-Zou-Shi” can decrease the toxicity of AA-containing herbs. We compared the three toxicities of AA standard,Madouling(anAristolochiaherb), and a herbal formula BFAJT. AA standard was given for BALB/c mice at a dose of 5 mg/kg bw/day or 7.5 mg/kg bw/day for 10 days.Madoulingand BFAJT were given at an equivalence of AA 0.5 mg/kg bw/day for 21 days. Nephrotoxicity was evaluated by metabolomics and histopathology. The urinary metabolomics profiles were characterized by1H NMR spectroscopy. The spectral data was analyzed with partial least squares discriminant analysis, and the significant differential metabolites between groups were identified. The result showed different degrees of acute renal tubular injuries, and metabolomics analysis found that the kidney injuries were focused in proximal renal tubules. Both metabolomics and pathological studies revealed that AA standard,Madouling, and BFAJT were all nephrotoxicants. The compositions of the compound remedy did not diminish the nephrotoxicity caused by AA.

scholarly journals Serum Metabolomic Profiling of Rats by Intervention of Aconitum soongaricum

2015 ◽  
Vol 10 (12) ◽  
pp. 1934578X1501001
Author(s):  
Fan Zhang ◽  
Jiao Liu ◽  
Jun Lei ◽  
Wenjing He ◽  
Yun Sun
Keyword(s):  
Statistical Analysis ◽  
Nmr Spectroscopy ◽  
Metabolic Profiles ◽  
Metabolomic Analysis ◽  
Serum Samples ◽  
Multivariate Statistical ◽  
H Nmr ◽  
Metabolomic Profiling ◽  
Clinical Detection ◽  
Endogenous Metabolites

To understand the toxic mechanism and to find the changes in the endogenous metabolites of Aconitum soongaricum Stapf for clinical detection, a combination of 1H NMR spectroscopy and multivariate statistical analysis was applied to examine the metabolic profiles of the blood serum samples collected from the rat model. In total, thirteen biomarkers of A. soongaricum were found and identified. It turned out that A. soongaricum treatment may partially disorder the metabolism. The study has shown the potential application of NMR-based metabolomic analysis in providing further insights into the toxicity caused by A. soongaricum.

Metabolomic analysis of serum by (1) H NMR spectroscopy in amyotrophic lateral sclerosis

2010 ◽  
Vol 411 (7-8) ◽  
pp. 563-567 ◽  
Author(s):  
Alok Kumar ◽  
Lakshmi Bala ◽  
Jayantee Kalita ◽  
U.K. Misra ◽  
R.L. Singh ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Nmr Spectroscopy ◽  
Metabolomic Analysis ◽  
H Nmr ◽  
Lateral Sclerosis

scholarly journals Metabolomic analysis with 1 H-NMR for non-invasive diagnosis of hepatic fibrosis degree in patients with chronic hepatitis C

2017 ◽  
Vol 49 (12) ◽  
pp. 1338-1344 ◽  
Author(s):  
Tommaso Gabbani ◽  
Maria Marsico ◽  
Patrizia Bernini ◽  
Elisabetta Lorefice ◽  
Cecilia Grappone ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Hepatic Fibrosis ◽  
Metabolomic Analysis ◽  
H Nmr ◽  
Non Invasive
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