Qualitative, quantitative, and pharmacokinetic study on the absorbed components of Ardisia japonica ( Thunb .) Blume in rat plasma based on molecular networking combined with quadrupole time‐of‐flight LC/MS and triple quadrupole LC/MS

2021 ◽  
Author(s):  
He Chen Wang ◽  
Tian Jiao Li ◽  
Yong Rui Bao ◽  
Shuai Wang ◽  
Xian Sheng Meng
Keyword(s):  
Pharmacokinetic Study ◽  
Time Of Flight ◽  
Rat Plasma ◽  
Triple Quadrupole ◽  
Molecular Networking

scholarly journals A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic Study

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Yi Li ◽  
Hui Guo ◽  
Yinying Wu ◽  
Qianqian Geng ◽  
Danfeng Dong ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Pharmacokinetic Study ◽  
High Performance ◽  
Time Of Flight ◽  
Internal Standard ◽  
High Sensitivity ◽  
Rat Plasma ◽  
Linear Range ◽  
Tandem Mass ◽  
Tandem Mass Spectrometric

A rapid and sensitive method for determining aesculin of Cortex fraxini in rat was developed using high-performance liquid chromatography (HPLC) quadrupole time-of-flight (QTOF) tandem mass (MS/MS). Rat plasma was pretreated by fourfold methanol to remove plasma proteins. Chromatographic separation was performed on a reverse phase column. A tandem mass spectrometric detection with an electrospray ionization (ESI) interface was achieved using collision-induced dissociation (CID) under positive ionization mode. The MS/MS patterns monitored werem/z341.2716 →m/z179.1043 for aesculin andm/z248.3025 →m/z120.9130 for tinidazole (internal standard). The linear range was calculated to be 10.0–1500.0 ng/mL with a detection limit of 2.0 ng/mL. The inter- and intraday accuracy and precision were within ±7.0%. Pharmacokinetic study showed that aesculin was confirmed to be a one-compartment open model. The method is believed to have good linear range, high sensitivity and recoveries, and superior analytical efficiency. It will probably be an alternative for pharmacokinetic study of aesculin.

scholarly journals Analysis of Bioactive Components, Simultaneous Determination and Pharmacokinetic Study of Six Components After Intragastric Administration of Qiling Wenshen Xiaonang Granule.

2020 ◽  
Author(s):  
Jiawen Shi ◽  
Yu Wang ◽  
Yiqian Zhang ◽  
Yuanxue Liu ◽  
Zhipeng Huo ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Polycystic Ovary ◽  
Rat Plasma ◽  
Intragastric Administration ◽  
Triple Quadrupole ◽  
Quadrupole Mass ◽  
Ionization Source ◽  
Significant Difference

Abstract Background: Qiling Wenshen Xiaonang Granule (QLWSG), a compound preparation of traditional Chinese medicine, which was recently approved by the National Medical Products Administration for clinical trials of polycystic ovary syndrome (PCOS). It is necessary to do further researches about QLWSG for better understanding.Methods: In this study, qualitative and quantitative analysis of QLWSG in rat plasma were conducted by ultra-fast liquid chromatography coupled with triple quadrupole mass spectrometry (UFLC-QqQ-MS). The multi-reaction monitoring mode/electrospray ionization source of the triple quadrupole mass spectrometer was used for detection. The plasma samples were prepared by precipitating protein with organic solvent.Results: 21 prototypes in rat plasma were identified after intragastric administration of QLWSG, and most of the identified components are flavonoids and phenolic acids. Furthermore, a sensitive and specific UFLC-QqQ-MS method was also built, which was used for simultaneous determination and pharmacokinetic study of six active components, astragaloside IV, calycosin, calycosin-7-O-β-D-glucoside, icariin, epimedin C and rosmarinic acid in rat plasma after intragastric administration of QLWSG with three doses (8, 16, 24 g/kg). All the calibration curves showed good linearity (r > 0.9942) in the range of concentration measured. The validated method is stable and reliable, meanwhile, successfully applied for pharmacokinetic evaluation of tested compounds. The results showed that there was no significant difference in half-lives (t1/2) and clearance (CL/F) of six analytes at the three doses were observed.Conclusions: It can be inferred that the pharmacokinetic behavior of QLWSG is positively correlated to dose at the range of 8–24 g/kg. Simultaneous determination of the six compounds in vivo for the first time by UFLC-QqQ-MS. The validated method and the results of this study would benefit therapeutic material basis and clinical application for QLWSG. And the validated method would also be applied to the analysis of other Chinese medicine compound prescriptions.

Quantification of annonacin in Rat plasma: Application to pharmacokinetic study

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
N Bonneau ◽  
I Schmitz Afonso ◽  
D Touboul ◽  
A Brunelle ◽  
P Champy
Keyword(s):  
Pharmacokinetic Study ◽  
Rat Plasma
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