Quantification of mycophenolic acid in human plasma by liquid chromatography with time‐of‐flight mass spectrometry for therapeutic drug monitoring

2020 ◽  
Author(s):  
Natalicia J. Antunes ◽  
Nina Ince ◽  
Jason Raymond ◽  
Karin Kipper ◽  
Lewis Couchman ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Therapeutic Drug Monitoring ◽  
Human Plasma ◽  
Mycophenolic Acid ◽  
Drug Monitoring ◽  
Time Of Flight ◽  
Therapeutic Drug ◽  
Flight Mass Spectrometry

scholarly journals Rapid liquid chromatography tandem mass spectrometry determination of rivaroxaban levels in human plasma for therapeutic drug monitoring

2017 ◽  
Vol 25 (2) ◽  
Author(s):  
Andreea Varga ◽  
Răzvan Constantin Șerban ◽  
Daniela Lucia Muntean ◽  
Cristina Maria Tătar ◽  
Lenard Farczadi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Therapeutic Drug Monitoring ◽  
Tandem Mass Spectrometry ◽  
Human Plasma ◽  
Drug Monitoring ◽  
Single Step ◽  
Therapeutic Drug ◽  
Tandem Mass ◽  
Limit Of Quantification

AbstractA rapid, sensitive, high-throughput liquid chromatography coupled with tandem mass spectrometry method for the quantification of rivaroxaban from human plasma has been developed and validated. For the analytical separation a Zorbax SB-C18 column with isocratic flow of mobile phase composed of 0.2% formic acid in water and acetonitril (65:35, V/V) with a flow rate of 1 mL/min at a temperature of 45ºC was used. Detection of rivaroxaban was performed using positive electrospray ionization and MS/MS mode (sum of m/z 231.1; 289.2 and 318.2 from m/z 436.3). Plasma samples were prepared using single-step protein precipitation with methanol. Method validation was performed with regards to selectivity, linearity (r >0.9927), within-run and between-run precision (CV< 13.1 %) and accuracy (bias< 9.4 %) over a concentration range of 24.00 - 960.00 ng/mL plasma. Recovery was between 96.5 - 108.5% and the lower limit of quantification of rivaroxaban was 24.00 ng/mL. The developed method is simple, rapid, and selective, requires small plasma sample volumes, and was successfully applied for therapeutic drug monitoring of rivaroxaban in treated patients.

Development of Sensitive and High-Throughput Liquid Chromatography–Tandem Mass Spectrometry Method for Quantification of Haloperidol in Human Plasma with Phospholipid Removal Pretreatment

2020 ◽  
Author(s):  
Nela Zidekova ◽  
Adam Nemcek ◽  
Martina Sutovska ◽  
Juraj Mokry ◽  
Martin Kertys
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Therapeutic Drug Monitoring ◽  
Tandem Mass Spectrometry ◽  
Human Plasma ◽  
Therapeutic Drug ◽  
Tandem Mass ◽  
Extraction Recovery ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Abstract Haloperidol, butyrophenone derivative, is a typical antipsychotic drug used in the treatment of schizophrenia, manic phase of bipolar disorder, and acute psychomotor agitations. According to the recent guidelines for therapeutic drug monitoring, it is strongly recommended to measure plasma level during the therapy with haloperidol. The objective of this study was to develop and validate a simple liquid chromatography–tandem mass spectrometry-based method to quantitate haloperidol in human plasma. After one-step extraction procedure using OSTROTM plate, gradient elution on Acquity UPLC BEH C18 (50 × 2.1 mm, 1.7 μm) column over 3.2 min was performed. The detection was conducted on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring mode in positive ionization mode with transitions at m/z 376.29 → 165.14 and m/z 380.28 → 169.17 for haloperidol and haloperidol-d4 (used as an internal standard), respectively. The method was fully validated to cover wide concentration range of 0.05–80 ng/mL in human plasma and meets the criteria for the selectivity, linearity and lower limit of detection, precision and accuracy, matrix effect, extraction recovery, carryover, dilution integrity and stability. The extraction recovery was nearly 100%, and no significant matrix effects were observed. Therefore, the method is applicable to routine therapeutic drug monitoring in patients’ plasma.

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