Simultaneous quantification and pharmacokinetic evaluation of roflumilast and its N‐oxide in cynomolgus monkey plasma by LC–MS/MS method

2020 ◽  
Author(s):  
Haijun Qu ◽  
Keyu Zhang ◽  
Chunqing Fu ◽  
Feng Qiu
Keyword(s):  
Cynomolgus Monkey ◽  
Simultaneous Quantification ◽  
Monkey Plasma ◽  
Pharmacokinetic Evaluation

Detection of Elevated Antibody Against Calreticulin by ELISA in Aged Cynomolgus Monkey Plasma

2011 ◽  
Vol 28 (2) ◽  
pp. 85-89 ◽  
Author(s):  
Atsunori Higashino ◽  
Takashi Kageyama ◽  
Sachi Sri Kantha ◽  
Keiji Terao
Keyword(s):  
Cynomolgus Monkey ◽  
Monkey Plasma

LC–MS/MS quantification of next-generation biotherapeutics: a case study for an IgE binding Nanobody in cynomolgus monkey plasma

Bioanalysis ◽  
2014 ◽  
Vol 6 (9) ◽  
pp. 1201-1213 ◽  
Author(s):  
Koen Sandra ◽  
Kjell Mortier ◽  
Lucie Jorge ◽  
Luis C Perez ◽  
Pat Sandra ◽  
...  
Keyword(s):  
Cynomolgus Monkey ◽  
Next Generation ◽  
Ige Binding ◽  
Monkey Plasma

scholarly journals Method development and validation for the quantitation of the complement inhibitor Cp40 in human and cynomolgus monkey plasma by UPLC-ESI-MS

2017 ◽  
Vol 1041-1042 ◽  
pp. 19-26 ◽  
Author(s):  
Alexandra Primikyri ◽  
Malvina Papanastasiou ◽  
Yiannis Sarigiannis ◽  
Sophia Koutsogiannaki ◽  
Edimara S. Reis ◽  
...  
Keyword(s):  
Cynomolgus Monkey ◽  
Method Development ◽  
Complement Inhibitor ◽  
Esi Ms ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Monkey Plasma

Comparison of Lipoprotein Separation and Lipid Analysis Methodologies for Human and Cynomolgus Monkey Plasma Samples

2011 ◽  
Vol 5 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Seongah Han ◽  
Amy M. Flattery ◽  
David McLaren ◽  
Richard Raubertas ◽  
Sang Ho Lee ◽  
...  
Keyword(s):  
Cynomolgus Monkey ◽  
Lipid Analysis ◽  
Plasma Samples ◽  
Monkey Plasma

scholarly journals Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLC/ESI-Q-TOF MS and Their Protective Effects on PC12 Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Erwei Hao ◽  
Jianfeng Qin ◽  
Wei Wei ◽  
Jianhua Miao ◽  
Yan Xie ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Pc12 Cells ◽  
Cynomolgus Monkey ◽  
Cell Damage ◽  
Neuroprotective Effect ◽  
Control Group ◽  
Protective Effects ◽  
Health Food ◽  
Monkey Plasma ◽  
Tof Ms

Yizhi Granule (YZG) is a health food containing six traditional Chinese medicines (TCMs). It improves memory barriers in rat experiments. Here, we describe the first fast and sensitive ultraperformance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF MS) method for analyzing YZG in plasma. We used this technique for studies in cynomolgus monkey plasma. By comparing retention time, MS, and MS/MS data of reference compounds, 70 compounds were detected in YZG. Of these, 63 were identified including 60 saponins, 2 flavones, and 1 methyl ester. There were 33 saponins, 1 flavone, and 1 methyl ester in the plasma. Next, to study the therapeutic properties of YZG, the neuroprotective effect of some of the absorbed components was evaluated using PC12 cell damage caused by the Aβ25–35 model. The results showed that 9 compounds protect PC12 cells from Aβ25–35 with cell viability (%) of 111.00 ± 8.12 (G-Rb1), 102.20 ± 4.22 (G-Rb2), 100.34 ± 6.47 (G-Rd), 102.83 ± 2.10 (G-Re), 101.68 ± 7.64 (NG-Fa), 101.19 ± 7.83 (NG-R1), 102.53 ± 0.55 (NG-R2), 106.88 ± 4.95 (gypenoside A), and 103.95 ± 4.11 (gypenoside XLIX), respectively, versus the control group (87.51 ± 6.59). These results can reveal the real pharmacodynamic basis of YZG and provide a theoretical basis for subsequent studies. It can also provide some references for the research of Alzheimer’s disease.

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