Development and validation of a reversed-phase liquid chromatographic method for separation and simultaneous determination of COX-2 inhibitors in pharmaceuticals and its application to biological fluids

2005 ◽  
Vol 19 (5) ◽  
pp. 362-368 ◽  
Author(s):  
R. Nageswara Rao ◽  
S. Meena ◽  
D. Nagaraju ◽  
A. Raghu Ram Rao
Keyword(s):  
Chromatographic Method ◽  
Biological Fluids ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Cox 2 ◽  
Development And Validation ◽  
Cox 2 Inhibitors ◽  
Liquid Chromatographic

scholarly journals Development and Validation of Liquid Chromatographic Method for Estimation of Ibuprofen and Famotidine in Combined Dosage Form

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Dimal A. Shah ◽  
Dixita J. Suthar ◽  
Sunil L. Baldania ◽  
Usman K. Chhalotiya ◽  
Kashyap K. Bhatt
Keyword(s):  
Mobile Phase ◽  
Dosage Form ◽  
Chromatographic Method ◽  
Reversed Phase ◽  
Assay Method ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Development And Validation ◽  
Liquid Chromatographic

An isocratic, reversed phase-liquid-chromatographic assay method was developed for the quantitative determination of ibuprofen and famotidine in combined-dosage form. A Brownlee C18, 5 μm column with mobile phase containing water : methanol : acetonitrile (30 : 60 : 10, v/v/v) was used. The flow rate was 1.0 mL/min, and effluents were monitored at 264 nm. The retention times of ibuprofen and famotidine were 4.9 min and 6.8 min, respectively. The linearity for ibuprofen and famotidine was in the range of 2–20 μg/mL and 0.1–10 μg/mL, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. The method was successfully applied to the estimation of ibuprofen and famotidine in combined dosage form.

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