Quantitative analysis of multicomponents by a single marker and quality evaluation of Venenum Bufonis from different geographical origins

2019 ◽  
Vol 33 (9) ◽  
Author(s):  
Ke‐ke Cui ◽  
Yue‐ting Cao ◽  
Jia‐feng Shao ◽  
Zheng‐yu Lu ◽  
Long‐hu Wang
Keyword(s):  
Quantitative Analysis ◽  
Quality Evaluation ◽  
Single Marker

scholarly journals Systematic Study on a Quantitative Analysis of Multicomponents by Single Marker (QAMS) Method for Simultaneous Determination of Eight Constituents in Pneumonia Mixture by UPLC-MS/MS

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Haibo Zhang ◽  
Weina Xie ◽  
Jiangyun Liu ◽  
Xiaoqiang Xiang ◽  
Shilei Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Quality Evaluation ◽  
Acute Bronchitis ◽  
Internal Reference ◽  
Relative Retention ◽  
External Standard Method ◽  
New Strategy ◽  
Single Marker ◽  
Good Repeatability ◽  
And Control

Pneumonia mixture was formulated and is available to treat children acute pneumonia and acute bronchitis in our hospital for nearly forty years, but there are few studies of its quality evaluation or control. In this paper, a new strategy for quality evaluation of pneumonia mixture was explored and verified through qualitative and quantitative analyses of multicomponents by single marker (QAMS) by UPLC-MS/MS. Baicalein was selected as an internal reference, and the relative correction factors (RCFs) and the relative retention time (RRT) of (R, S)-goitrin, amygdalin, chlorogenic acid, pseudoephedrine hydrochloride, ephedrine hydrochloride, ammonium glycyrrhizinate, and baicalin were established. The robustness and durability of the QAMS method were investigated. RCF values calculated by the average (AVG) method and linear regression (LRG) method had good repeatability and were acceptable for quantitative analysis, and the RTT combined with the exact masses of precursor and fragment ions and their abundance could be adopted for accurately positioning the chromatographic peak of the eight constituents. The consistency and feasibility of the QAMS method were verified by comparing the contents of the seven components calculated by a classic and validated external standard method (ESM) with those of the QAMS method, which reduces analytical cost and time of detection and avoids the problem of the diversity and large quantity of reference standards. The results demonstrated that the QAMS method developed in this paper could provide a new, alternative, and promising method to comprehensively and effectively determine multicomponents and control the quality of pneumonia mixture or even a group of similar medicines.

scholarly journals Chromatographic fingerprinting and quantitative analysis for the quality evaluation of Xinfeng capsule

2020 ◽  
pp. 1-7
Author(s):  
Leilei Gao ◽  
Fang Wang ◽  
Mei Meng
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Quality Evaluation ◽  
Hierarchical Cluster ◽  
Array Detector ◽  
Diode Array Detector ◽  
Chromatographic Fingerprint ◽  
Xinfeng Capsule ◽  
Single Marker ◽  
The Common

Abstract A simple, accurate and sensitive method of high performance liquid chromatography (HPLC) with diode array detector was established to identify Xinfeng capsules and systematically evaluated its quality, based on chromatographic fingerprint integrated with the similarity analysis, hierarchical cluster analysis and the quantitative analysis of multi-components by single marker (QAMS). In this study, 18 peaks were selected as the common peaks to evaluate the similarities among different batches (S1–S10) of Xinfeng capsules samples, which were manufactured in the First Affiliated Hospital of Anhui University of Chinese Medicine with a three-year span. Compared to control fingerprint, the similarities values for 10 batches of samples were more than 0.90. Moreover, by analyzing the reference of astragalus, the chromatogram of astragalus was developed, and 10 common peaks of astragalus were identified. More importantly, simultaneous quantification of three markers in Xinfeng capsule, including Calycosin-7-glucoside, calycosin and Formononetinaldehyde was performed, the three constituents showed good regression (R > 0.999) within linear ranges, and their recoveries were within the range of 97.6–101.5%. The validation results showed that the developed method was specific, accurate, precise and robust. This study demonstrated that the developed method offers an efficient, reliable and practical approach for systematic quality evaluation of Xinfeng capsule.

scholarly journals Simultaneous Determination of Six Active Components in Danggui Kushen Pills via Quantitative Analysis of Multicomponents by Single Marker

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Huiwei Bao ◽  
Jihong Chi ◽  
Huailei Yang ◽  
Fangxin Liu ◽  
Kuo Fang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Gallic Acid ◽  
Quality Evaluation ◽  
Evaluation Method ◽  
Internal Standard ◽  
Active Components ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker

In this paper, a valid evaluation method for the quality control of Danggui Kushen pills (DKP) has been established based on quantitative analysis of multicomponents by single marker (QAMS). Gallic acid, matrine, oxymatrine, catechin, ferulic acid, and rutin were selected as the indexes for quality evaluation of DKP. The analysis was achieved on an Agilent ZORBAX SB-C18 column (250  mm × 4.6  mm, 5 μm) via gradient elution. Gallic acid was used as internal standard to determine the relative correction factors (RCF) between gallic acid and other five constituents in DKP. The contents of those components were calculated at the same time. The accuracy of QAMS method was verified by comparing the contents of six components calculated by external standard (ES) method with those of the QAMS method. It turned out that there was no significant difference between the quantitative results of QAMS method and external standard method. The proposed QAMS method was proved to be accurate and feasible according to methodological experiments, which provided an accurate, efficient, and economical approach for quality evaluation of DKP.

Quality Evaluation of Astragali Radix Products by Quantitative Analysis of Multi-components by Single Marker

2013 ◽  
Vol 5 (4) ◽  
pp. 272-279 ◽  
Author(s):  
Jie SUN ◽  
Zhen-zuo JIANG ◽  
Rui-qing YAN ◽  
Olajide OLALEYE ◽  
Xiao-long ZHANG ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Quality Evaluation ◽  
Astragali Radix ◽  
Single Marker
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