Application of a sensitive and specific LC-ESI-MS/MS method for the simultaneous quantification of twelve bioactive components in dog plasma for an intravenous pharmacokinetic study of Yiqifumai Injection in beagle dogs

2018 ◽  
Vol 32 (8) ◽  
pp. e4256
Author(s):  
Fugeng Zhang ◽  
Xiaoli Qiao ◽  
Hui Lu ◽  
Shaoqiang Zhang ◽  
Wuxun Du ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Bioactive Components ◽  
Beagle Dogs ◽  
Esi Ms ◽  
Simultaneous Quantification ◽  
Dog Plasma

LC-ESI-MS/MS determination of 4-methylpyrazole in dog plasma and its application to a pharmacokinetic study in dogs

2017 ◽  
Vol 32 (2) ◽  
pp. e4065 ◽  
Author(s):  
Devaraj V. Chandrasekhar ◽  
Ponnayyan Sulochana Suresh ◽  
Sreekanth Dittakavi ◽  
Rakesh A. Hiremath ◽  
Ravi Kanth Bhamidipati ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Esi Ms ◽  
Dog Plasma

Sensitive LC-MS/MS Method for the Simultaneous Determination of Bendamustine and its Active Metabolite, γ-Hydroxybendamustine in Small Volume Mice and Dog Plasma and its Application to a Pharmacokinetic Study in Mice and Dogs

Drug Research ◽  
2017 ◽  
Vol 67 (09) ◽  
pp. 497-508 ◽  
Author(s):  
Devaraj Chandrashekar ◽  
Ponnayyan Suresh ◽  
Rajnish Kumar ◽  
Ravi Bhamidipati ◽  
Ramesh Mullangi ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Small Volume ◽  
Internal Standard ◽  
Simultaneous Quantification ◽  
Liquid Liquid Extraction ◽  
Dog Plasma ◽  
Highly Sensitive ◽  
Regulatory Guidelines ◽  
Novel Method

AbstractA highly sensitive, specific and rapid LC-ESI-MS/MS method has been developed and validated for the simultaneous quantification of bendamustine (BM) and γ-hydroxybendamustine (HBM) in small volume (20 µL) mice and dog plasma using phenacetin as an internal standard (IS) as per regulatory guidelines. Both the analytes and IS were extracted from mice and dog plasma using a liquid-liquid extraction method. Chromatography was achieved on Atlantis dC18 column using an isocratic mobile phase (0.2% formic acid:acetonitrile, 25:75) at a flow rate of 0.40 mL/min. The total chromatographic run time was 3.0 min and the elution of BM, HBM and IS occurred at ~1.2, 1.2 and 2.0 min, respectively. A linear response function was established 0.11–518 ng/mL for both the analytes in mice and dog plasma. The intra- and inter-day accuracy and precisions were in the range of 3.46–12.9 and 3.63–8.23%; 1.15–9.00 and 7.86–9.49% for BM and HBM, respectively in mice plasma and 2.15–6.49 and 1.73–13.1%; 4.35–13.9 and 4.33–10.5% for BM and HBM, respectively in dog plasma. This novel method has been applied to a pharmacokinetic study in mice and dogs.

Rapid and simultaneous quantification of seven bioactive components in Radix Astragali based on pressurized liquid extraction combined with HPLC-ESI-MS/MS analysis

2015 ◽  
Vol 7 (7) ◽  
pp. 3054-3062 ◽  
Author(s):  
Feng Qiu ◽  
Zhanqi Tong ◽  
Jianyi Gao ◽  
Manyuan Wang ◽  
Muxin Gong
Keyword(s):  
Liquid Extraction ◽  
Pressurized Liquid Extraction ◽  
Radix Astragali ◽  
Bioactive Components ◽  
Running Time ◽  
Esi Ms ◽  
Simultaneous Quantification ◽  
Ms Analysis

The running time of analyzing one sample using the developed HPLC-ESI-MS/MS method is only 4.5 min, and more than 150 samples could be assayed daily.

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