Simultaneous determination of 10 kinds of biogenic amines in rat plasma using high-performance liquid chromatography coupled with fluorescence detection

2018 ◽  
Vol 32 (6) ◽  
pp. e4211 ◽  
Author(s):  
Xinna Wang ◽  
Yawei Liang ◽  
Yaqi Wang ◽  
Mingqin Fan ◽  
Yanni Sun ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biogenic Amines ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
High Performance ◽  
Rat Plasma

scholarly journals Simultaneous Determination of Bergapten, Imperatorin, Notopterol, and Isoimperatorin in Rat Plasma by High Performance Liquid Chromatography with Fluorescence Detection and Its Application to Pharmacokinetic and Excretion Study after Oral Administration of Notopterygium incisum Extract

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
John Teye Azietaku ◽  
Xie-an Yu ◽  
Jin Li ◽  
Jia Hao ◽  
Jun Cao ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
High Performance ◽  
Internal Standard ◽  
Rat Plasma ◽  
Fluorescence Detector ◽  
Excretion Study

A specific, sensitive, and reliable high performance liquid chromatography with fluorescence detection (HPLC-FLD) was first optimized and then used in the simultaneous quantification of bergapten, imperatorin, notopterol, and isoimperatorin in rat plasma using osthole as the internal standard. Liquid-liquid extraction with ethyl acetate was employed in treating the rat plasma samples obtained. Separation was carried out with a Hedera™ ODS column (4.6 × 250 mm, 5 μm) by gradient elution at a temperature of 40°C. Excitation and emission of the fluorescence detector were set to 300 and 490 nm, respectively. The lower limits of quantification for bergapten, imperatorin, notopterol, and isoimperatorin in rat plasma were 4, 40, 4, and 2 ng mL−1, respectively. The intraday and interday precision and accuracy for the four coumarins were within acceptable criteria. The recovery of the method was satisfactory with a range of 80.3–114%. The validated method was successfully used for the simultaneous determination of the four coumarins in Notopterygium incisum extracts and also for the pharmacokinetic and excretion study of bergapten, imperatorin, notopterol, and isoimperatorin in rats.

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