Direct injection HPLC micro method for the determination of voriconazole in plasma using an internal surface reversed-phase column

2004 ◽  
Vol 18 (9) ◽  
pp. 719-722 ◽  
Author(s):  
F. Péhourcq ◽  
C. Jarry ◽  
B. Bannwarth
Keyword(s):  
Direct Injection ◽  
Internal Surface ◽  
Reversed Phase ◽  
Phase Column ◽  
Reversed Phase Column

A Non-Extraction HPLC Method for the Simultaneous Determination of Serum Paracetamol and Salicylate

1988 ◽  
Vol 25 (6) ◽  
pp. 661-667 ◽  
Author(s):  
C M Dawson ◽  
T W M Wang ◽  
S J Rainbow ◽  
T R Tickner
Keyword(s):  
Benzoic Acid ◽  
Direct Injection ◽  
Internal Standard ◽  
Internal Surface ◽  
Reversed Phase ◽  
Hplc Method ◽  
Good Precision ◽  
Phase Column ◽  
Reversed Phase Column

A direct injection HPLC method for the sumultaneous measurement of serum paracetamol and salicylate is described using a Pinkerton internal surface reversed-phase column with benzoic acid as internal standard. The method is linear to at least 1000 mg/L for both drugs and shows good precision at levels of 62–500 mg/L. None of the drugs tested for interference affected the quantitation of either drug. In patient samples, the values obtained with this method correlated well with those from enzymatic paracetamol and Trinder salicylate methods.

Improved liquid-chromatographic determination of caffeine in plasma.

1980 ◽  
Vol 26 (9) ◽  
pp. 1351-1354 ◽  
Author(s):  
J Blanchard ◽  
J D Mohammadi ◽  
K A Conrad
Keyword(s):  
Plasma Sample ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Small Sample ◽  
Small Animals ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Phase Column ◽  
Reversed Phase Column

Abstract We describe a rapid, specific, and sensitive liquid-chromatographic micro-method for caffeine in plasma. Each plasma sample can be assayed within about 15 min of its receipt. Samples are denatured with acetonitrile, centrifuged, and the supernate is chromatographed on a reversed-phase column. Only 100 microL of plasma is required, and concentrations as low as 0.3 mg/L can be measured accurately. Other xanthines and their metabolites do not interfere. The small sample required makes the procedure ideally suited for measuring caffeine in the plasma of infants and small animals as well as adults.

scholarly journals Rapid Postcolumn Methodology for Determination of Paralytic Shellfish Toxins in Shellfish Tissue

2008 ◽  
Vol 91 (3) ◽  
pp. 589-597 ◽  
Author(s):  
Wade A Rourke ◽  
Cory J Murphy ◽  
Ginette Pitcher ◽  
Jeffery M van de Riet ◽  
B Garth Burns ◽  
...  
Keyword(s):  
Reversed Phase ◽  
Mouse Bioassay ◽  
Working Standard ◽  
Shellfish Toxins ◽  
Paralytic Shellfish Toxins ◽  
Paralytic Shellfish ◽  
Relative Standard ◽  
Phase Column ◽  
Reversed Phase Column

Abstract A rapid liquid chromatographic (LC) method with postcolumn oxidation and fluorescence detection (excitation 330 nm, emission 390 nm) for the determination of paralytic shellfish toxins (PSTs) in shellfish tissue has been developed. Extracts prepared for mouse bioassay (MBA) were treated with trichloroacetic acid to precipitate protein, centrifuged, and pH-adjusted for LC analysis. Saxitoxin (STX), neoSTX (NEO), decarbamoylSTX (dcSTX), and the gonyautoxins, GTX1, GTX2, GTX3, GTX4, GTX5, dcGTX2, and dcGTX3, were separated on a polar-linked alkyl reversed-phase column using a step gradient elution; the N-sulfocarbamoyl GTXs, C1, C2, C3, and C4, were determined on a C-8 reversed-phase column in the isocratic mode. Relative toxicities were used to determine STX-dihydrochloride salt (diHCl) equivalents (STXeq). Calibration graphs were linear for all toxins studied with STX showing a correlation coefficient of 0.999 and linearity between 0.18 and 5.9 ng STX-diHCl injected (equivalent to 3.9128 g STXeq/100 g in tissue). Detection limits for individual toxins ranged from 0.07 g STXeq/100 g for C1 and C3 to 4.1 g STXeq/100 g for GTX1. Spike recoveries ranged from 76 to 112 in mussel tissue. The relative standard deviation (RSD) of repeated injections of GTX and STX working standard solutions was <4. Uncertainty of measurement at a level of 195 g STXeq/100 g was 9, and within-laboratory reproducibility expressed as RSD was 4.6 using the same material. Repeatability of a 65 g STXeq/100 g sample was 3.0 RSD. Seventy-three samples were analyzed by the new postcolumn method and both AOAC Official Methods for PST determination: the MBA (y = 1.22x + 13.99, r2 = 0.86) and the precolumn LC oxidation method of Lawrence (y = 2.06x + 12.21, r2 = 0.82).

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