Determination of sunitinib and its active metabolite,N-desethyl sunitinib in mouse plasma and tissues by UPLC-MS/MS: assay development and application to pharmacokinetic and tissue distribution studies

2014 ◽  
Vol 29 (5) ◽  
pp. 679-688 ◽  
Author(s):  
Xiao Chen ◽  
Zhong Wang ◽  
Mengping Liu ◽  
Min Liao ◽  
Xinfeng Wang ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Active Metabolite ◽  
Assay Development ◽  
Mouse Plasma ◽  
Distribution Studies

scholarly journals Determination of Epimedin B in Rat Plasma and Tissue by LC-MS/MS: Application in Pharmacokinetic and Tissue Distribution Studies

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Qianru Feng ◽  
Shunjun Xu ◽  
Jiejing Yu ◽  
Shuai Sun ◽  
Liu Yang
Keyword(s):  
Tissue Distribution ◽  
Rat Plasma ◽  
Precipitation Method ◽  
Tissue Homogenate ◽  
Spectrometric Method ◽  
Female Rat ◽  
Mass Spectrometric Method ◽  
Tandem Mass Spectrometric ◽  
Distribution Studies

A simple, sensitive, and specific liquid chromatography tandem mass-spectrometric method was developed and validated for the determination of epimedin B in rat plasma and tissue samples. After being processed with a protein precipitation method, these samples were separated on an Agilent Eclipse XDB-C18 column with an isocratic mobile phase consisting of acetonitrile and 0.1% formic acid aqueous solution (32 : 68, v/v). The calibration curve of epimedin B was linear over the concentration range from 1 to 500 ng/mL in plasma and tissue homogenate. The method was then applied to pharmacokinetic and tissue distribution studies after a single oral administration of Herba Epimedii extract to SD rats. Results showed that epimedin B reached the plasma peak concentration at 0.4 h and the terminal elimination half-life was 1.6 h in rat plasma, and the plasma area under the curve from time zero to infinity (AUC0–∞) was 14.35 μg/L·h. The concentration distribution of epimedin B in rat tissue was in the following order: liver > ovary > womb > lung > kidney > spleen > heart > brain, indicating that the compound could be widely distributed in rat, and the reproductive system may be the principal target of epimedin B for female rat.

scholarly journals Determination of Oxaliplatin by a UHPLC-MS/MS Method: Application to Pharmacokinetics and Tongue Tissue Distribution Studies in Rats

2021 ◽  
Vol 15 (1) ◽  
pp. 52
Author(s):  
Xiuqing Gao ◽  
Robert Y. L. Tsai ◽  
Jing Ma ◽  
Yang Wang ◽  
Xiaohua Liu ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Lower Limit ◽  
Limit Of Detection ◽  
Rat Plasma ◽  
Patch Application ◽  
Icp Ms ◽  
Platinum Based Chemotherapy ◽  
Limit Of Quantitation ◽  
Distribution Studies

Oxaliplatin (OXP), a third-generation platinum-based chemotherapy drug, was often indirectly analyzed via total platinum by an ICP-MS because it was difficult to directly quantify using an LC-MS/MS method, due to its instability, bad column separability and severe MS signal inhibition. Here, we developed and validated a specific, sensitive and reproducible LC-MS/MS method for the quantification of OXP itself in rat plasma and tongue tissue on a SCIEX 4000 QTRAP® MS/MS system equipped with a Phenomenex Lux 5u Cellulose-1 column (250 × 4.6 mm, 5 μm). This method was validated at the lower limit of detection (LOD) and the lower limit of quantitation (LLOQ) of 5 ng/mL and 10 ng/mL, with linearity of 10–5000 ng/mL (r2 > 0.99) and 10–2500 ng/mL (r2 > 0.99), in rat plasma and tongue homogenates, respectively. The intra- and inter-day precision (CV%) and accuracy (RE%) were within 15% for LLOQ, low-, medium- and high-quality control samples. The mean extraction recoveries were around 50% and 80% for plasma and tongue homogenates, respectively. This assay was successfully applied to pharmacokinetics study following intravenous administration of OXP, as well as tongue tissue distribution after 1 h and 4 h of a novel oral mucosal patch application.

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