Effect of realgar on extracellular amino acid neurotransmitters in hippocampal CA1 region determined by online microdialysis-dansyl chloride derivatization-high-performance liquid chromatography and fluorescence detection

2014 ◽  
Vol 28 (9) ◽  
pp. 1254-1262 ◽  
Author(s):  
Taoguang Huo ◽  
Yinghua Zhang ◽  
Weikai Li ◽  
Huilei Yang ◽  
Hong Jiang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Fluorescence Detection ◽  
High Performance ◽  
Amino Acid Neurotransmitters ◽  
Ca1 Region ◽  
Hippocampal Ca1 Region ◽  
Hippocampal Ca1 ◽  
Extracellular Amino Acid

scholarly journals A Jerte Valley Cherry-Based Product as a Supply of Tryptophan

2012 ◽  
Vol 5 ◽  
pp. IJTR.S9394 ◽  
Author(s):  
María Garrido ◽  
Javier Espino ◽  
Antonio F. Toribio-Delgado ◽  
Javier Cubero ◽  
Juan I. Maynar-Mariño ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Bioactive Compounds ◽  
Fluorescence Detection ◽  
Essential Amino Acid ◽  
High Performance ◽  
Good Source ◽  
Potential Health ◽  
Tryptophan Content

L-Tryptophan (tryptophan) is an essential amino acid in humans. It has important roles as a precursor of different bioactive compounds. Based on previous studies in which tryptophan has been shown to be present in fresh cherries, the aim of the present work was to analyze the tryptophan content of a Jerte Valley cherry-based product. A previously optimized method of analysis of tryptophan was used, ie, high-performance liquid chromatography with fluorescence detection (HPLC/FL). As expected, HPLC/FL technique permitted to detect and quantify the tryptophan content in a different matrix rather than fresh cherries. In fact, the Jerte Valley cherry-based product contained 69.54 ± 10.64 ppm of tryptophan, thereby showing that this product is a good source of tryptophan. In summary, it has been proven that the Jerte Valley cherry-based product is rich in tryptophan and may be indicated as a supply of this essential amino acid as well as having potential health benefits for conditions where tryptophan is necessary.

scholarly journals Determination of amino acid profile for argininosuccinic aciduria disorder using High-Performance Liquid Chromatography with fluorescence detection

2020 ◽  
Author(s):  
Hossein Salmanizadeh ◽  
Neda Sahi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Fluorescence Detection ◽  
Mobile Phase ◽  
High Performance ◽  
Potassium Dihydrogen Phosphate ◽  
Amino Acid Profile ◽  
Dihydrogen Phosphate ◽  
Argininosuccinic Aciduria

Argininosuccinic aciduria is an autosomal, recessive amino acid disorder that is caused by a deficiency of the argininosuccinate lyase enzyme. Citrulline is the most significant marker to detect this disorder. We used the High-performance liquid chromatography with fluorescence detection with 450 nm emission and 330 nm excitation wavelengths, 15 mmol/L potassium dihydrogen phosphate and 5 mmol/L dipotassium hydrogen phosphate as Mobile Phase A, and 50 mL water, 250 mL acetonitrile, and 200 mL methanol as Mobile Phase B in gradient mode with flow rate of 1.2 mL/min. The citrulline concentration was 22 µmol/L in healthy infants and 220 µmol/L in infants suffering from the disorder.

Sign in / Sign up

Export Citation Format

Share Document