Development of solid-phase extraction method and its application for determination of hydrochlorothiazide in human plasma using HPLC

2004 ◽  
Vol 18 (2) ◽  
pp. 71-76 ◽  
Author(s):  
Dragica Zendelovska ◽  
Trajče Stafilov ◽  
Petar Miloševski
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Extraction Method ◽  
Solid Phase ◽  
Phase Extraction

A new and simple solid-phase extraction method for LC determination of pyronaridine in human plasma

2005 ◽  
Vol 824 (1-2) ◽  
pp. 45-50 ◽  
Author(s):  
S RAMANATHAN ◽  
S KARUPIAH ◽  
N NAIR ◽  
P OLLIARO ◽  
V NAVARATNAM ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Extraction Method ◽  
Solid Phase ◽  
Phase Extraction

scholarly journals Determination of Ranitidine in Human Plasma by SPE and ESI-LC-MS/MS for Use in Bioequivalence Studies

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Karini B. Bellorio ◽  
Maria Isabel R. Alves ◽  
Nelson R. Antoniosi Filho
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Extraction Method ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Internal Standard ◽  
Phase Extraction ◽  
Good Separation ◽  
Limit Of Quantification

A method for determining ranitidine in human plasma by ESI-LC-MS/MS was validated, using propranolol as internal standard. The extraction method used was solid phase extraction (SPE). Chromatographic separation was performed in a Chromolith C18 (50 mm × 4.6 mm i.d.) analytical column, which provided good separation of ranitidine and propranolol peaks with an analysis time of 2.5 minutes. Extraction yields of 94.4% for ranitidine and 89.4% for the internal standard were obtained. The lower limit of quantification (LLOQ) was 3.00 ng/mL, and limit of detection (LOD) was 0.05 ng/mL, with linearity ranging from 3.00 to 500 ng/mL. The results, thus, showed that this method is suitable for application in bioequivalence studies of ranitidine in human plasma.

scholarly journals A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography

2019 ◽  
Vol 7 (11) ◽  
pp. 1757-1761 ◽  
Author(s):  
Dragica Zendelovska ◽  
Emilija Atanasovska ◽  
Kalina Gjorgjievska ◽  
Kristina Pavlovska ◽  
Krume Jakjovski ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Extraction Method ◽  
High Performance ◽  
Solid Phase ◽  
Hplc Method ◽  
Phase Extraction ◽  
Good Precision ◽  
Plasma Samples

BACKGROUND: A new simple, selective and accurate high-performance liquid chromatographic (HPLC) method utilising solid-phase extraction for the determination of pantoprazole in human plasma samples has been developed. AIM: The purpose of this paper was developing a new HPLC method suitable for the determination of pantoprazole in plasma samples, which enables simple and rapid isolation and concentration of the analysed drug.METHODS: The chromatographic separation was accomplished on a LiChroCart LiChrospher 60 RP select B column using a mobile phase composed of 0.2 % (V/V) water solution of triethylamine (pH 7) and acetonitrile (58:42, V/V) followed by UV detection was at 280 nm. The solid-phase extraction method using LiChrolut RP-18 (200 mg, 3 ml) was applied to the obtained good separation of investigated drug from endogenous plasma components. Best results were achieved when plasma samples were buffered with 0.1 mol/L KH2PO4 (pH 9) before extraction, eluted and reconstituted with acetonitrile and 0.001 mol/L NaOH after extraction, respectively. RESULTS: The standard calibration curves showed good linearity within the range of 25.0-4000.0 ng/mL with a correlation coefficient greater than 0.996. Retention times of pantoprazole and internal standard, lansoprazole was 4.1 and 6.0 min respectively. The limit of quantification was 25.0 ng/mL. For intra- and inter-day precision relative standard deviations ranged from 4.2 to 9.3%. The relative errors for stability investigations were ranged from 0.12 to -10.5%. CONCLUSION: This method has good precision and accuracy and was successfully applied to the pharmacokinetic and bioequivalence study of 40 mg pantoprazole in healthy volunteers.

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