Validated HPLC method for the quantitative determination of CoQ10 in dog plasma and its application to a pharmacokinetic study

2010 ◽  
Vol 25 (9) ◽  
pp. 1038-1044 ◽  
Author(s):  
Bo Yuan ◽  
Chunling Liu ◽  
Pingwei Xu ◽  
Lin Lin ◽  
Cheng Pan ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Pharmacokinetic Study ◽  
Hplc Method ◽  
Dog Plasma

Validated HPLC method for quantitative determination of talinolol in rat plasma and application to a preclinical pharmacokinetic study

Bioanalysis ◽  
2010 ◽  
Vol 2 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Shriram M Pathak ◽  
Prashant B Musmade ◽  
Krishnamurthy M Bhat ◽  
Nayanabhirama Udupa
Keyword(s):  
Quantitative Determination ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Hplc Method

QUANTITATIVE DETERMINATION OF CURCUMIN IN TURMERIC POWDER AND TURMERIC–HONEY BALL PILLS BY HPLC

2018 ◽  
Vol 8 (4) ◽  
pp. 42-47
Author(s):  
Tien Nguyen Huu ◽  
Tram Le Thi Bao ◽  
Ngoc Nguyen Thi Nhu ◽  
Thang Phan Phuoc ◽  
Khan Nguyen Viet
Keyword(s):  
Acetic Acid ◽  
Gastric Mucosa ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
Curcuma Longa ◽  
Biological Marker ◽  
Hplc Method ◽  
Chromatography Analysis ◽  
Precision And Accuracy

Background: Curcumin is a major ingredient in turmeric (Curcuma longa L., Zingiberaceae), which has important activities such as anti-tumor, anti-inflammatory, antioxidant, anti-ischemia, protection of gastric mucosa etc,. Curcumin can be considered as a biological marker of turmeric and turmeric products. Objectives: Developing an HPLC method for quantification of curcumin in turmeric powder and turmeric - honey ball pills; applying this method for products on the market. Materials and methods: turmeric powder and turmeric - honey ball pills collected in Thua Thien Hue province. After optimization process, the method was validated and applied to evaluate the content of curcumin. Results: The chromatography analysis was performed with: Zorbaz Eclipse XDB-C18 (150 × 4.6 nm; 5 µm); Mobile phase: acetonitril: 2% acetic acid (45:55), Flow rate was kept constant at 1.0 ml/min; Detector PDA (420 nm). The method was validated for the HPLC system compatibility, specificity, linearity range, precision and accuracy; the recovery greater than 98%. Conclusion: The developed HPLC method can determine curcumin in turmeric powder and turmeric - honey ball pills. Key words: Curcumin, turmeric powder, turmeric-honey ball pills, quantitative determination, HPLC

scholarly journals A new selective, and sensitive method for the determination of lixivaptan, a vasopressin 2 (V2)-receptor antagonist, in mouse plasma and its application in a pharmacokinetic study

2018 ◽  
Vol 16 (1) ◽  
pp. 614-620
Author(s):  
Haitham Alrabiah ◽  
Mohammed Abunassif ◽  
Sabry Attia ◽  
Gamal Abdel-Hafiz Mostafa
Keyword(s):  
Receptor Antagonist ◽  
Pharmacokinetic Study ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Hplc Method ◽  
Maximum Plasma ◽  
Mouse Plasma ◽  
V2 Receptor ◽  
V2 Receptor Antagonist

AbstractA new, selective and sensitive HPLC method for the determination of lixivaptan, an oral selective vasopressin 2 (V2)-receptor antagonist, was investigated and validated. A Waters symmetry C18 column was used as a stationary phase in isocratic elution mode using a mobile phase composed of KH2PO4 (100 mM)-acetonitrile (40: 60, v/v) at a flow rate of 1.5 mL min-1. Diclofenac was used as the internal standard (IS). Lixivaptan and the IS were extracted from plasma by protein precipitation and were detected at 260 nm. Lixivaptan and diclofenac were eluted at 3.6 and 6.2 min, respectively. The developed method showed good linearity over the calibration range of 50 -1000 ng mL-1 with a lower limit of detection of 16.5 ng mL-1. The extraction percentage of lixivaptan in the mouse plasma was in the range of 88.88 - 114.43%, which indicates acceptable extraction. The aforementioned method was validated according to guidelines of the International Council on Harmonization (ICH). The intra- and inter-day coefficients of variation did not exceed 5.5%. This method was presented to be simple, sensitive, and accurate and was successfully adapted in a pharmacokinetic study of the profile of lixivaptan in mouse plasma. A mean maximum plasma concentration of lixivaptan of 113.82 ng mL-1 was achieved in 0.5 h after oral administration of a 10 mg kg-1 dose in mouse as determined using the developed method.

LC-ESI-MS/MS determination of 4-methylpyrazole in dog plasma and its application to a pharmacokinetic study in dogs

2017 ◽  
Vol 32 (2) ◽  
pp. e4065 ◽  
Author(s):  
Devaraj V. Chandrasekhar ◽  
Ponnayyan Sulochana Suresh ◽  
Sreekanth Dittakavi ◽  
Rakesh A. Hiremath ◽  
Ravi Kanth Bhamidipati ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Esi Ms ◽  
Dog Plasma
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