Identification of chemical constituents in Rhizoma Paridis Saponins and their oral administration in rat plasma by UPLC/Q-TOF/MS

2010 ◽  
Vol 25 (6) ◽  
pp. 712-719 ◽  
Author(s):  
Shuli Man ◽  
Wenyuan Gao ◽  
Yanjun Zhang ◽  
Luqi Huang ◽  
Changxiao Liu
Keyword(s):  
Oral Administration ◽  
Chemical Constituents ◽  
Rat Plasma ◽  
Tof Ms

Analysis of the constituents in the rat plasma after oral administration of Yin Chen Hao Tang by UPLC/Q-TOF-MS/MS

2008 ◽  
Vol 46 (3) ◽  
pp. 477-490 ◽  
Author(s):  
Xijun Wang ◽  
Wenjun Sun ◽  
Hui Sun ◽  
Haitao Lv ◽  
Zeming Wu ◽  
...  
Keyword(s):  
Oral Administration ◽  
Rat Plasma ◽  
Tof Ms

Rapid discovery and identification of multiple absorbed chemical constituents and metabolites in rat cerebrospinal fluid after oral administration of Jitai tablets by a LC-MS based metabolomics approach

RSC Advances ◽  
2016 ◽  
Vol 6 (39) ◽  
pp. 32811-32822 ◽  
Author(s):  
Shuping Wang ◽  
Peng Fu ◽  
Lei Liu ◽  
Lingling Wang ◽  
Chengcheng Peng ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Statistical Analysis ◽  
Oral Administration ◽  
Chemical Constituents ◽  
Tof Ms

An integrative strategy using LC-Q/TOF-MS and LC-QqQ-MS/MS coupled with multi-variate statistical analysis was developed to screen and identify the constituents transported into the rat cerebrospinal fluid after oral administration of Jitai tablets.

scholarly journals Comprehensive characterization of the chemical constituents in Yiganmingmu oral liquid and the absorbed prototypes in cynomolgus monkey plasma after oral administration by UPLC-Q-TOF-MS based on the self built components database

2021 ◽  
Author(s):  
wei wei ◽  
Siwei Li ◽  
Linyou Cheng ◽  
Erwei Hao ◽  
Xiaotao Hou ◽  
...  
Keyword(s):  
Oral Administration ◽  
Phenolic Acids ◽  
Cynomolgus Monkey ◽  
Chemical Constituents ◽  
Herbal Medicines ◽  
The Self ◽  
Triterpenoid Saponins ◽  
Oral Liquid ◽  
Tof Ms

Abstract Background: Yiganmingmu oral liquid (YGMM), a well known over-the-counter (OTC) drug in China, is composed of 12 types of valuable herbal medicines and has been widely used in clinical for the treatment of soreness and weakness of waist and knees, dizziness, memory loss, and fatigue. However, the chemical compositions of YGMM and its absorbed compounds in plasma remain unclear. Methods: Since chemical investigation is the first important step to reveal effects and action mechanisms of traditional Chinese medicine (TCM), in this study, based on the self built components database, systematic characterization of the chemical profile of YGMM in vivo was carried out by using a reliable UPLC-Q-TOF-MS method. Moreover, to obtain better understanding of the absorbed prototypes in plasma, serum pharmacochemistry analysis of YGMM after oral administration was conducted by using cynomolgus monkeys as animal model. Results: A total of 667 constituents from the 12 single herbal medicines were collected in the self built components database by searching the reported literatures, and 415 of them were initially screened as candidate compounds in YGMM by comparison of their experimental accurate mass measurements with those theoretical values. After that, 117 compounds including 17 phenolic acids, 25 flavonoids, 4 alkaloids, 10 phthalides, 5 monoterpenes, 8 triterpenoid saponins, 9 anthraquinones, and 39 other compounds, were unambiguously identified or tentatively characterized by analysing their MS/MS fragmentation patterns, and also by comparison with reference standards and those data reported in the literatures. 61 prototypes absorbed in plasma of cynomolgus monkey, including 13 phenolic acids, 21 flavonoids, 8 phthalides, 3 monoterpenes, 4 triterpenoid saponins, and 12 other compounds were tentatively assigned by serum pharmacochemistry analysis after oral administration. Conclusion: It was the first comprehensive analysis of chemical constituents of YGMM and prototypes in plasma, and the data analysis strategy developed in this study showed high efficiency in the structural elucidations. The results might provide scientific evidence for further research on material basis of YGMM.

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