High-performance liquid chromatographic enantioseparation of (R,S)-fluoxetine using Marfey's reagent and (S)-N-(4-nitrophenoxycarbonyl) phenylalanine methoxyethyl ester as chiral derivatizing reagents along with direct thin-layer chromatographic resolutio

2010 ◽  
Vol 24 (11) ◽  
pp. 1152-1158 ◽  
Author(s):  
Ravi Bhushan ◽  
Charu Agarwal
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Marfey’S Reagent ◽  
Marfey's Reagent ◽  
Chiral Derivatizing Reagents ◽  
Liquid Chromatographic

Liquid-chromatographic estimation of saturated phospholipid palmitate in amniotic fluid compared with a thin-layer chromatographic method for acetone-precipitated lecithin.

1982 ◽  
Vol 28 (11) ◽  
pp. 2264-2268 ◽  
Author(s):  
S M Sax ◽  
J J Moore ◽  
A Oley ◽  
J S Amenta ◽  
J A Silverman
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
High Performance ◽  
Chromatographic Method ◽  
Dynamic Range ◽  
Fetal Lung ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Phenacyl Esters ◽  
Liquid Chromatographic

Abstract The "high-performance" liquid-chromatographic method we report for measuring palmitate derived from saturated phospholipids in amniotic fluid is sensitive and precise and possesses a wide dynamic range. Palmitate from nonsurfactant sources is largely excluded. The procedure involves oxidation of the chloroform/methanol-extracted lipids with osmium tetroxide, precipitation of the resulting phosphatides with cold acetone, mild alkaline hydrolysis, derivatization of the liberated fatty acids to phenacyl esters, and reversed-phase chromatography. The method is no less convenient or more time-consuming than measurement of the lecithin/sphingomyelin ratio by thin-layer chromatography. The clinical usefulness of the procedure is indicated by results of a correlative study of 26 amniotic fluids evaluated by "lung profile" tests in a second laboratory. A value for dipalmitoyl lecithin of 13 mg/L or greater indicates fetal lung maturity.

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