Development and validation of an analytical method for the quantification of cytochrome c in skin transport studies

2009 ◽  
Vol 24 (7) ◽  
pp. 732-736 ◽  
Author(s):  
Yogeshwar G. Bachhav ◽  
Yogeshvar N. Kalia
Keyword(s):  
Cytochrome C ◽  
Analytical Method ◽  
Transport Studies ◽  
Development And Validation ◽  
Skin Transport

Development and Validation of an HPLC Method for Determination of Spirotetramat and Spirotetramat cis enol in Various Vegetables and Soil

2013 ◽  
Vol 96 (3) ◽  
pp. 670-675 ◽  
Author(s):  
Balwinder Singh ◽  
Kousik Mandal ◽  
Sanjay K Sahoo ◽  
Urvashi Bhardwaj ◽  
Raminderjit Singh Battu
Keyword(s):  
Analytical Method ◽  
Anhydrous Sodium Sulfate ◽  
Hplc Method ◽  
Array Detector ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Precision And Accuracy ◽  
Hplc Grade Acetonitrile ◽  
Development And Validation

Abstract An easy and simple analytical method was standardized and validated for the estimation of residues of spirotetramat and its metabolite spirotetramat cis enol in various substrates: okra fruits, brinjal leaves and fruits, green chili, red chili, and soil. The samples were extracted with acetonitrile, diluted with brine solution, partitioned into dichloromethane, dried over anhydrous sodium sulfate, and cleaned up by treatment with activated charcoal powder. Final clear extracts were concentrated under vacuum and reconstituted with HPLC grade acetonitrile. Residues were estimated using HPLC with a photodiode array detector and a C18 column, and confirmed by HPTLC. Acetonitrile was used as the mobile phase at 0.4 mL/min. Both spirotetramat and spirotetramat cis enol presented distinct peak at retention times of 8.518 and 7.598 min, respectively. Consistent recoveries ranging from 82 to 97% for spirotetramat and spirotetramat cis enol were observed when samples were spiked at 1.00 to 0.03 mg/kg levels. The LOQ of the method was found to be 0.03 mg/kg. The analytical method was validated in terms of parameters, including selectivity, linearity, precision, and accuracy.

Development and Validation of a Green Analytical Method for the Determination of Norfloxacin in Raw Material by FTIR Spectrophotometry

2016 ◽  
Vol 99 (6) ◽  
pp. 1533-1536 ◽  
Author(s):  
Jéssica Sayuri Hisano Natori ◽  
Eliane Gandolpho Tótoli ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Urinary Tract ◽  
Organic Solvents ◽  
Analytical Method ◽  
Environmentally Friendly ◽  
Raw Material ◽  
Waste Generation ◽  
Ftir Spectrophotometry ◽  
Tract Infections ◽  
Development And Validation

Abstract Norfloxacin is a broad-spectrum antimicrobial agent, widely used in humans and animals for the treatment of urinary tract infections. It is a second-generation fluoroquinolone. Several analytical methods to analyze norfloxacin have been described in the literature. However, most of them are complex and require the use of large amounts of organic solvents. This paper describes the development and validation of a green analytical method for the determination of norfloxacin in raw material by FTIR spectrophotometry. This method does not require the use of organic solvents, minimizing waste generation in the process and its environmental impacts. The development of methods that promote the reduction, prevention, or elimination of waste generation has become highly attractive to the pharmaceutical industry because of the growing demand from civil society and government authorities for environmentally friendly products and services. The FTIR spectrophotometry method was validated according to International Conference on Harmonization guidelines, showing adequate linearity (r = 0.9936), precision, accuracy, and robustness. This validated method can be used as an environmentally friendly alternative for the quantification of norfloxacin in raw material in QC routine analysis.

Development and Validation of an Analytical Method Readily Applicable for Quality Control of Tabebuia impetiginosa (Taheebo) Ethanolic Extract

2018 ◽  
Vol 101 (3) ◽  
pp. 695-700 ◽  
Author(s):  
Yan Jin ◽  
Kyung Min Jeong ◽  
Jeongmi Lee ◽  
Jing Zhao ◽  
Su-Young Choi ◽  
...  
Keyword(s):  
Quality Control ◽  
Analytical Method ◽  
Ethanolic Extract ◽  
Development And Validation

scholarly journals Oxcarbazepine: validation and application of an analytical method

2010 ◽  
Vol 46 (2) ◽  
pp. 265-272 ◽  
Author(s):  
Paula Cristina Rezende Enéas ◽  
Renata Barbosa de Oliveira ◽  
Gerson Antônio Pianetti
Keyword(s):  
Spectrophotometric Method ◽  
Analytical Method ◽  
Disintegration Time ◽  
International Conference ◽  
Pharmacopoeial Monograph ◽  
Development And Validation ◽  
Excipient Compatibility

Oxcarbazepine (OXC) is an important anticonvulsant and mood stabilizing drug. A pharmacopoeial monograph for OXC is not yet available and therefore the development and validation of a new analytical method for quantification of this drug is essential. In the present study, a UV spectrophotometric method for the determination of OXC was developed. The various parameters, such as linearity, precision, accuracy and specificity, were studied according to International Conference on Harmonization Guidelines. Batches of 150 mg OXC capsules were prepared and analyzed using the validated UV method. The formulations were also evaluated for parameters including drug-excipient compatibility, flowability, uniformity of weight, disintegration time, assay, uniformity of content and the amount of drug dissolved during the first hour.

scholarly journals Development and validation of an analytical method using UPLC–MS/MS to quantify everolimus in dried blood spots in the oncology setting

2018 ◽  
Vol 149 ◽  
pp. 106-113 ◽  
Author(s):  
Lotte M. Knapen ◽  
Yvo de Beer ◽  
Roger J.M. Brüggemann ◽  
Leo M. Stolk ◽  
Frank de Vries ◽  
...  
Keyword(s):  
Analytical Method ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Development And Validation

Development and Validation of an Analytical Method for Betanine and Isobetanine in Processed Food Products Labeled with Beet Red

2021 ◽  
Vol 36 (5) ◽  
pp. 376-381
Author(s):  
Hyun-Hee Kang ◽  
◽  
Choong-In Yun ◽  
Gayeong Lee ◽  
Jae-Wook Shin ◽  
...  
Keyword(s):  
Analytical Method ◽  
Food Products ◽  
Processed Food ◽  
Development And Validation
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