A rapid HPLC/ESI-MS/MS method for quantitative analysis of isovalerylshikonin in rat plasma

2009 ◽  
pp. n/a-n/a
Author(s):  
MeiXiang Zhu ◽  
Yanbing Gao ◽  
Zhen Wu ◽  
YuFen Zhao
Keyword(s):  
Quantitative Analysis ◽  
Rat Plasma ◽  
Esi Ms

Revealing changes in curcumin bioavailability using vitamin C as an enhancer by HPLC-MS/MS

2019 ◽  
Vol 16 ◽  
Author(s):  
Xufen Dai ◽  
Jiaxue Hao ◽  
Ying Feng ◽  
Jing Wang ◽  
Qiannan Li ◽  
...  
Keyword(s):  
Vitamin C ◽  
High Performance ◽  
Internal Standard ◽  
Fold Increase ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Solution Ph ◽  
Esi Ms ◽  
Simple Strategy ◽  
Isolated Compound

Background: Curcumin (CUR), a natural isolated compound from turmeric, has been the promising star in fighting many diseases but the broad application of curcumin has been limited ascribed to low bioavailability. Objective: The aim of this study is to pursue the enhancement of curcumin bioavailability through co-administration of vitamin C. Methods: Such purpose was achieved through the analysis of curcumin pharmacokinetics by high performance liquid chromatography coupled with electrospray ionization - tandem mass spectrometry (HPLC - ESI - MS/MS). The plasma was separated on a C18 reverse phase column using acetonitrile and ammonium formate solution (pH 6.5; 2.0 mM) at 0.8 mL/min. MS/MS detection was carried out in negative mode using mass patterns of m/z 367.0 > 216.7 for curcumin and m/z 265.2 > 223.9 for internal standard (honokiol). Results: Successful application of the proposed method in the pharmacokinetic study presented clear changes in key pharmacokinetic parameters including the growth of AUC (0-t) up to 2.4 times, 2.2-fold increase of Cmax, 2.2-fold loss of CL, and 1.5-fold diminishment of t1/2. Conclusion: We developed an HPLC-ESI-MS/MS method for determination of curcumin in rat plasma and validated the improvement of bioavailability of curcumin through co-administration of vitamin C. We reasoned these changes to the inhibition of lipid peroxidation induced by the use of vitamin C. Such a simple strategy is possible to become an alternative for enhancing curcumin efficiency in practice.

A profluorescent nitroxide probe for ascorbic acid detection and its application to quantitative analysis of diabetic rat plasma

RSC Advances ◽  
2016 ◽  
Vol 6 (65) ◽  
pp. 60907-60915 ◽  
Author(s):  
Yuta Matsuoka ◽  
Kei Ohkubo ◽  
Toshihide Yamasaki ◽  
Mayumi Yamato ◽  
Hiroshi Ohtabu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Quantitative Analysis ◽  
Rat Plasma ◽  
Diabetic Rat ◽  
Nitroxide Probe ◽  
Ascorbic Acid Detection

15-((9-(Ethylimino)-10-methyl-9Hbenzo[a]phenoxazin-5-yl)amino)-3,11-dioxa-7-azadispiro[5.1.58.36]hexadecan-7-yloxyl, (Nile-DiPy) has been synthesized and examined as an off–on profluorescent nitroxide probe for measuring ascorbic acid in plasma.

Development of quantitative analytical method for volatile thiol compound with LC-ESI-MS as nonvolatile derivative by integrating a thiol-specific derivatization

2021 ◽  
Author(s):  
Yusuke Kawano ◽  
Kengo Suzuki ◽  
Iwao Ohtsu
Keyword(s):  
Quantitative Analysis ◽  
Electrospray Ionization ◽  
Efficient Method ◽  
Analytical Method ◽  
Coffee Bean ◽  
Thiol Compound ◽  
Esi Ms ◽  
Volatile Thiols

Abstract Generally, volatile thiols are hard to be measured with ESI (electrospray ionization)-type LC-MS due to the volatility. Therefore, we here evaluated the pretreatment of their S-bimanyl derivatization by monobromobimane to enable the detection as nonvolatile derivative. Consequently, we successfully developed the convenient and efficient method through the quantitative analysis of 2-furanmethanethiol (volatile thiol odorant of coffee aroma) in coffee bean.

Enantioselective LC‐ESI‐MS/MS determination of dropropizine enantiomers in rat plasma and application to a pharmacokinetic study

2018 ◽  
Vol 33 (3) ◽  
pp. e4434 ◽  
Author(s):  
Narayanan Balaji ◽  
Bhavesh Babulal Gabani ◽  
Umesh Todmal ◽  
Suresh P. Sulochana ◽  
Neeraj Kumar Saini ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Esi Ms

scholarly journals Ion-exchange chromatography followed by ESI-MS for quantitative analysis of sugar monophosphates from glucose catabolism

2006 ◽  
Vol 17 (1) ◽  
pp. 104-107 ◽  
Author(s):  
James J. Walters ◽  
Michael A. Grayson ◽  
Michael L. Gross ◽  
Maureen Hughes ◽  
Georgia Shearer ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Esi Ms ◽  
Glucose Catabolism
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