A reversed phase liquid chromatographic method for the simultaneous determination of several common penicillins in human serum

1991 ◽  
Vol 5 (2) ◽  
pp. 78-82 ◽  
Author(s):  
E. Mendez-Alvarez ◽  
R. Soto-Otero ◽  
G. Sierra-Paredes ◽  
E. Aguilar-Veiga ◽  
J. Galan-Valiente ◽  
...  
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Chromatographic Method ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Liquid Chromatographic

scholarly journals Validated Reversed-Phase Liquid Chromatographic Method with Gradient Elution for Simultaneous Determination of the Antiviral Agents: Sofosbuvir, Ledipasvir, Daclatasvir, and Simeprevir in Their Dosage Forms

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4611
Author(s):  
Essam Ezzeldin ◽  
Nisreen F. Abo-Talib ◽  
Marwa H. Tammam ◽  
Yousif A. Asiri ◽  
Abd El-Galil E. Amr ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Chromatographic Method ◽  
Limit Of Detection ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Dosage Forms ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Liquid Chromatographic

A simple, rapid, sensitive, and precise reversed-phase liquid chromatographic method was developed and validated for the simultaneous determination of four direct-acting antivirals, sofosbuvir (SF), ledipasvir (LD), declatasvir (DC), and simeprevir (SM), in their respective pharmaceutical formulations. Effective chromatographic separation was achieved on an Agilent Eclipse plus C8 column (250 mm × 4.6 mm, 5 µm) at 40 °C with gradient elution using a mobile phase composed of acetonitrile:phosphate buffer (pH 6.5). The quantification of SF and DC was based on peak area measurements at 260 nm, while the quantification of LD and SM was achieved at 330 nm. The linearity was acceptable from 1.0 to 20.0 μg/mL for the studied drugs, with correlation coefficients >0.999. The analytical performance of the newly proposed HPLC procedure was thoroughly validated according to ICH guidelines in terms of linearity, precision (RSD%, 0.39–1.57), accuracy (98.05–101.90%), specificity, limit of detection (LOD) (0.022–0.039 μg/mL), limit of quantification (LOQ) (0.067–0.118 μg/mL), and robustness. The validated HPLC method was successfully used to analyze the abovementioned drugs in their pure and dosage forms without interference from common excipients present in commercial formulations.

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