Ischaemic conditioning reduces kidney injury in an experimental large-animal model of warm renal ischaemia

2015 ◽  
Vol 102 (12) ◽  
pp. 1517-1525 ◽  
Author(s):  
J. P. Hunter ◽  
S. A. Hosgood ◽  
A. D. Barlow ◽  
M. L. Nicholson
Keyword(s):  
Animal Model ◽  
Kidney Injury ◽  
Large Animal Model ◽  
Large Animal ◽  
Renal Ischaemia ◽  
Ischaemic Conditioning

A Large Animal Model for Acute Kidney Injury by Temporary Bilateral Renal Artery Occlusion

10.3791/62230 ◽  
2021 ◽  
Author(s):  
Ilias P. Doulamis ◽  
Alvise Guariento ◽  
Mossab Y. Saeed ◽  
Rio S. Nomoto ◽  
Thomas Duignan ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Animal Model ◽  
Renal Artery ◽  
Kidney Injury ◽  
Artery Occlusion ◽  
Large Animal Model ◽  
Large Animal ◽  
Renal Artery Occlusion ◽  
Bilateral Renal Artery

scholarly journals Effect of Low-Frequency Therapeutic Ultrasound on Induction of Nitric Oxide in CKD: Potential to Prevent Acute Kidney Injury

2020 ◽  
Vol 6 (6) ◽  
pp. 453-460
Author(s):  
Michael W. Dae ◽  
Kathleen D. Liu ◽  
Richard J. Solomon ◽  
Dong W. Gao ◽  
Carol A. Stillson
Keyword(s):  
Nitric Oxide ◽  
Acute Kidney Injury ◽  
Animal Model ◽  
Blood Flow ◽  
Radionuclide Imaging ◽  
Therapeutic Ultrasound ◽  
Low Frequency ◽  
Kidney Injury ◽  
Large Animal Model ◽  
Large Animal

<b><i>Introduction:</i></b> Post-contrast acute kidney injury (PC-AKI) develops in a significant proportion of patients with CKD after invasive cardiology procedures and is strongly associated with adverse outcomes. <b><i>Objective:</i></b> We sought to determine whether increased intrarenal nitric oxide (NO) would prevent PC-AKI. <b><i>Methods:</i></b> To create a large animal model of CKD, we infused 250 micron particles into the renal arteries in 56 ± 8 kg pigs. We used a low-frequency therapeutic ultrasound device (LOTUS – 29 kHz, 0.4 W/cm<sup>2</sup>) to induce NO release. NO and laser Doppler probes were used to assess changes in NO content and blood flow. Glomerular filtration rate (GFR) was measured by technetium-diethylene-triamine-pentaacetic acid (Tc-99m-DTPA) radionuclide imaging. PC-AKI was induced by intravenous infusion of 7 cm<sup>3</sup>/kg diatrizoate. In patients with CKD, we measured GFR at baseline and during LOTUS using Tc-99m--DTPA radionuclide imaging. <b><i>Results:</i></b> In the pig model, CKD developed over 4 weeks (serum creatinine [Cr], mg/dL, 1.0 ± 0.2–2.6 ± 0.9, <i>p</i> &#x3c; 0.01, <i>n</i> = 12). NO and renal blood flow (RBF) increased in cortex and medulla during LOTUS. GFR increased 75 ± 24% (<i>p</i> = 0.016, <i>n</i> = 3). PC-AKI developed following diatrizoate i.v. infusion (Cr 2.6 ± 0.7 baseline to 3.4 ± 0.6 at 24 h, <i>p</i> &#x3c; 0.01, <i>n</i> = 3). LOTUS (starting 15 min prior to contrast and lasting for 90 min) prevented PC-AKI in the same animals 1 week later (Cr 2.5 ± 0.4 baseline to 2.6 ± 0.7 at 24 h, <i>p</i> = ns, <i>n</i> = 3). In patients with CKD (<i>n</i> = 10), there was an overall 25% increase in GFR in response to LOTUS (<i>p</i> &#x3c; 0.01). <b><i>Conclusions:</i></b> LOTUS increased intrarenal NO, RBF, and GFR and prevented PC-AKI in a large animal model of CKD, and significantly increased GFR in patients with CKD. This novel approach may provide a noninvasive nonpharmacological means to prevent PC-AKI in high-risk patients.

Method used to establish a large animal model of drug-induced acute kidney injury

2021 ◽  
pp. 153537022098175
Author(s):  
Si-Yang Wang ◽  
Chao-Yang Zhang ◽  
Guang-Yan Cai ◽  
Xiang-Mei Chen
Keyword(s):  
Acute Kidney Injury ◽  
Animal Model ◽  
Health Hazard ◽  
Kidney Injury ◽  
Basic Research ◽  
Large Animal Model ◽  
Large Animal ◽  
Drug Induced ◽  
Large Animal Models ◽  
New Treatments

Acute kidney injury is a serious health hazard disease due to its complex etiology and lack of effective treatments, resulting in high medical costs and high mortality. At present, a large number of basic research studies on acute kidney injury have been carried out. However, acute kidney injury models established in rodents sometimes do not simulate the course of human disease well. Research in large animal models of acute kidney injury is relatively rare, and methods to build a mature model of acute kidney injury have failed. Because its kidney anatomy and morphology are very similar to those in humans, the mini pig is an ideal animal in which to model kidney disease. Nephrotoxic drug-induced acute kidney injury has a high incidence. In this study, we established models of acute kidney injury induced by two drugs (gentamicin and cisplatin). Finally, the model of cisplatin-induced acute kidney injury was developed successfully, but we found the model of gentamycin-induced acute kidney injury was not reproducible. Compared to other models, these models better represent acute kidney injury caused by antibiotics and chemotherapeutic drugs and provide a basis for the study of new treatments for acute kidney injury in a large animal model.

Zinc preconditioning protects against renal ischaemia reperfusion injury in a preclinical sheep large animal model

BioMetals ◽  
2018 ◽  
Vol 31 (5) ◽  
pp. 821-834 ◽  
Author(s):  
Dermot O’Kane ◽  
Luke Gibson ◽  
Clive N. May ◽  
Justin du Plessis ◽  
Arthur Shulkes ◽  
...  
Keyword(s):  
Animal Model ◽  
Reperfusion Injury ◽  
Large Animal Model ◽  
Large Animal ◽  
Ischaemia Reperfusion Injury ◽  
Renal Ischaemia ◽  
Ischaemia Reperfusion

DEVELOPMENT OF A LARGE ANIMAL MODEL OF OPIATE ADDICTION TO EVALUATE CARDIOVASCULAR FUNCTION.

Analgesia ◽  
1995 ◽  
Vol 1 (4) ◽  
pp. 598-602 ◽  
Author(s):  
L.D. Napier ◽  
Z. Mateo ◽  
D.A. Yoshishige ◽  
B.A. Barron ◽  
J.L. Caffrey
Keyword(s):  
Animal Model ◽  
Cardiovascular Function ◽  
Large Animal Model ◽  
Opiate Addiction ◽  
Large Animal

scholarly journals A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Premila D. Leiphrakpam ◽  
Hannah R. Weber ◽  
Andrea McCain ◽  
Roser Romaguera Matas ◽  
Ernesto Martinez Duarte ◽  
...  
Keyword(s):  
Animal Model ◽  
Acute Respiratory Distress Syndrome ◽  
Respiratory Distress Syndrome ◽  
Distress Syndrome ◽  
Inhalation Injury ◽  
Smoke Inhalation ◽  
Large Animal Model ◽  
Large Animal ◽  
X Ray ◽  
Chest X Ray

Abstract Background Acute respiratory distress syndrome (ARDS) is multifactorial and can result from sepsis, trauma, or pneumonia, amongst other primary pathologies. It is one of the major causes of death in critically ill patients with a reported mortality rate up to 45%. The present study focuses on the development of a large animal model of smoke inhalation-induced ARDS in an effort to provide the scientific community with a reliable, reproducible large animal model of isolated toxic inhalation injury-induced ARDS. Methods Animals (n = 21) were exposed to smoke under general anesthesia for 1 to 2 h (median smoke exposure = 0.5 to 1 L of oak wood smoke) after the ultrasound-guided placement of carotid, pulmonary, and femoral artery catheters. Peripheral oxygen saturation (SpO2), vital signs, and ventilator parameters were monitored throughout the procedure. Chest x-ray, carotid, femoral and pulmonary artery blood samples were collected before, during, and after smoke exposure. Animals were euthanized and lung tissue collected for analysis 48 h after smoke inhalation. Results Animals developed ARDS 48 h after smoke inhalation as reflected by a decrease in SpO2 by approximately 31%, PaO2/FiO2 ratio by approximately 208 (50%), and development of bilateral, diffuse infiltrates on chest x-ray. Study animals also demonstrated a significant increase in IL-6 level, lung tissue injury score and wet/dry ratio, as well as changes in other arterial blood gas (ABG) parameters. Conclusions This study reports, for the first time, a novel large animal model of isolated smoke inhalation-induced ARDS without confounding variables such as cutaneous burn injury. Use of this unique model may be of benefit in studying the pathophysiology of inhalation injury or for development of novel therapeutics.

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