Cyclin/proliferation cell nuclear antigen immunohistochemistry does not improve the prognostic power of Dukes' or Jass' classifications for colorectal cancer

1995 ◽  
Vol 82 (2) ◽  
pp. 184-187 ◽  
Author(s):  
J. P. Neoptolemos ◽  
G. D. Oates ◽  
K. M. Newbold ◽  
M. Robson ◽  
C. McConkey ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Nuclear Antigen ◽  
Proliferation Cell Nuclear Antigen ◽  
Prognostic Power

scholarly journals Circ-BANP Contributes to Cell Carcinogenesis and Aerobic Glycolysis by Regulating MAPK1 Through miR-874-3p in Colorectal Cancer

2021 ◽  
Author(s):  
Yunxin Zhang ◽  
Kexin Shen ◽  
Hanyi Zha ◽  
Wentao Zhang ◽  
Haishan Zhang
Keyword(s):  
Colorectal Cancer ◽  
Cell Proliferation ◽  
Aerobic Glycolysis ◽  
Lactate Production ◽  
Xenograft Model ◽  
Mitogen Activated Protein Kinase ◽  
Cell Counting ◽  
Nuclear Antigen ◽  
Luciferase Reporter

Abstract BackgroundCircular RNA-BTG3 associated nuclear protein (circ-BANP) was identifified to involve in cell proliferation of colorectal cancer (CRC). The aerobic glycolysis is a key metabolism mediating cancer progression. However, the role of circ-BANP on aerobic glycolysis in CRC remains unknown. MethodsThe expression of circ-BANP, microRNA (miR)-874-3p, and mitogen-activated protein kinase 1 (MAPK1) mNRA was detected using quantitative real-time polymerase chain reaction. Cell viability and invasion were measured by cell counting kit-8 assay or transwell assay. Glucose consumption and lactate production were assessed by a glucose and lactate assay kit. XF Extracellular Flux Analyzer was used to determine extracellular acidifification rate (ECAR). Western blot was used to analyze the levels of hexokinase-2 (HK2), pyruvate kinase M2 (PKM2), MAPK1, proliferating cell nuclear antigen (PCNA), Cyclin D1, N-cadherin, E-cadherin, hypoxia inducible factor-1α (HIF-1α), glucose transport protein 1(GLUT1), and c-Myc. The interaction between miR-874-3p and circ-BANP or MAPK1 was confifirmed by dual luciferase reporter assay. In vivo experiments were conducted through the murine xenograft model. ResultsCirc-BANP was up-regulated in CRC tissues and cell lines. Circ-BANP knockdown suppressed CRC cell proliferation, invasion and aerobic glycolysis in vitro as well as inhibited tumor growth in vivo. Circ-BANP was a sponge of miR-874-3p and performed anti-tumor effffects by binding to miR-874-3p in CRC cells. Subsequently, we confifirmed MAPK1 was a target of miR-874-3p and circ-BANP indirectly regulated MAPK1 expression by sponging miR-874-3p. After that, we found MAPK1 overexpression partially reversed circ-BANP deletion-mediated inhibition on cell carcinogenesis and aerobic glycolysis in CRC. ConclusionCirc-BANP accelerated cell carcinogenesis and aerobic glycolysis by regulating MAPK1 through miR- 874-3p in CRC, suggesting a promising therapeutic strategy for CRC treatment.

Pan cycle expression of proliferating cell nuclear antigen in human colorectal cancer and its proliferative correlations

Cytometry ◽  
1995 ◽  
Vol 22 (3) ◽  
pp. 190-199 ◽  
Author(s):  
Rachael M. Sawtell ◽  
David A. Rew ◽  
Robin N. Stradling ◽  
George D. Wilson
Keyword(s):  
Colorectal Cancer ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Human Colorectal Cancer ◽  
Proliferating Cell

scholarly journals Identification of Prognostic Biomarker Signatures and Candidate Drugs in Colorectal Cancer: Insights from Systems Biology Analysis

2018 ◽  
Author(s):  
Md. Rezanur Rahman ◽  
Tania Islam ◽  
Esra Gov ◽  
Beste Turanli ◽  
Md. Shahjaman ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Drug Repositioning ◽  
Molecular Biomarker ◽  
Cell Cycles ◽  
Pathway Enrichment ◽  
Protein Protein Interaction ◽  
Network Analyses ◽  
Microarray Datasets ◽  
Prognostic Power ◽  
Hub Proteins

Background and objectives: Colorectal cancer (CRC) is the 2nd most cause of cancer related death in the world, but early diagnosis ameliorates the survival of CRC. This report directed to identify molecular biomarker signatures in CRC. Materials and Methods: We analyzed two microarray datasets (GSE35279 and GSE21815) to identify common differentially expressed genes (DEGs). We performed functional overrepresentation, pathway enrichment, protein-protein interaction (PPI), reporter biomolecules, survival, and drug repositioning analyses were done on common DEGs. Results: Total 727 up-regulated and 99 down-regulated DEGs were detected. The significantly enriched pathways PI3K-Akt signaling, Wnt signaling, ECM-interaction, cell cycles were identified. The 10 hub proteins (ADNP, CCND1, CD44, CDK4, CEBPB, CENPA, CENPH, CENPN, MYC, and RFC2) were selected as proteomic signatures from PPI network. Analyses revealed 10 reporter transcription factors (ETS1, ESR1, GATA1, GATA2, GATA3, AR, YBX1, FOXP3, E2F4, and PRDM14) and 2 reporter microRNAs (miR-193b-3p and miR-615-3p) as regulatory component. The prognostic power analysis revealed that hub proteins and reporter biomolecules related with worse survival of patients in CRC. Several candidate repositioned drugs including anti-neoplastic and immunomodulating agents were identified using Connectivity map (CMap) and geneXpharma tool. Conclusions: This study presents biomarker signatures at protein and RNA levels with prognostic capability in CRC. We think that the molecular signatures and candidate drugs presented in this study can be potential biomarkers and therapeutic target in CRC.

scholarly journals Colorectal cancer-associated nuclear antigen

2000 ◽  
Vol 1501 (2-3) ◽  
pp. 162-170 ◽  
Author(s):  
Piotr Szymczyk ◽  
Jarosław Jakubik ◽  
Wanda M. Krajewska ◽  
Danuta Duś ◽  
Jan Berner ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Nuclear Antigen
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