Cloning and Characterization of a Panel of Mitochondrial Targeting Sequences for Compartmentalization Engineering in Saccharomyces cerevisiae

2021 ◽  
Author(s):  
Chang Dong ◽  
Zhuwei Shi ◽  
Lei Huang ◽  
Huimin Zhao ◽  
Zhinan Xu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Targeting ◽  
Mitochondrial Targeting Sequences

scholarly journals Characterization of the signal that directs Bcl-xL, but not Bcl-2, to the mitochondrial outer membrane

2003 ◽  
Vol 160 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Thomas Kaufmann ◽  
Sarah Schlipf ◽  
Javier Sanz ◽  
Karin Neubert ◽  
Reuven Stein ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Mitochondrial Outer Membrane ◽  
Mitochondrial Targeting ◽  
Mitochondrial Localization ◽  
Intracellular Membranes ◽  
Bona Fide ◽  
Targeting Signal ◽  
Tm Domain ◽  
Mitochondrial Targeting Sequences

It is assumed that the survival factors Bcl-2 and Bcl-xL are mainly functional on mitochondria and therefore must contain mitochondrial targeting sequences. Here we show, however, that only Bcl-xL is specifically targeted to the mitochondrial outer membrane (MOM) whereas Bcl-2 distributes on several intracellular membranes. Mitochondrial targeting of Bcl-xL requires the COOH-terminal transmembrane (TM) domain flanked at both ends by at least two basic amino acids. This sequence is a bona fide targeting signal for the MOM as it confers specific mitochondrial localization to soluble EGFP. The signal is present in numerous proteins known to be directed to the MOM. Bcl-2 lacks the signal and therefore localizes to several intracellular membranes. The COOH-terminal region of Bcl-2 can be converted into a targeting signal for the MOM by increasing the basicity surrounding its TM. These data define a new targeting sequence for the MOM and propose that Bcl-2 acts on several intracellular membranes whereas Bcl-xL specifically functions on the MOM.

Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes

1986 ◽  
Vol 6 (12) ◽  
pp. 4509-4515
Author(s):  
M Rosenkrantz ◽  
T Alam ◽  
K S Kim ◽  
B J Clark ◽  
P A Srere ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Citrate Synthase ◽  
Amino Terminus ◽  
Translation Product ◽  
Mitochondrial Targeting ◽  
E Coli ◽  
Homologous Genes ◽  
Primary Translation Product ◽  
Mitochondrial Targeting Sequences

Saccharomyces cerevisiae contains two genes, CIT1 and CIT2, encoding functional citrate synthase (K.-S. Kim, M. S. Rosenkrantz, and L. Guarente, Mol. Cell. Biol. 6:1936-1942, 1986). We show here that CIT2 encodes a nonmitochondrial form of citrate synthase. The DNA sequence of CIT2 presented provides a possible explanation for why the CIT2 product, unlike the CIT1 product, fails to be imported into mitochondria. While the products of these two genes are highly homologous, they diverge strikingly at their amino termini. The amino terminus of the CIT1 primary translation product extends 39 residues beyond the amino termini of Escherichia coli and porcine citrate synthases. This extension consists of a typical mitochondrial targeting motif. The amino terminus of the CIT2 primary translation product extends 20 residues beyond the amino termini of the E. coli and porcine enzymes. The CIT2-encoded extension is not homologous to that of CIT1, resulting in a nonmitochondrial localization of the product. The CIT2-encoded extension, however, does bear certain similarities to mitochondrial targeting sequences. The possible role of this sequence in targeting this CIT2 product to a nonmitochondrial organelle is discussed.

scholarly journals Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes.

1986 ◽  
Vol 6 (12) ◽  
pp. 4509-4515 ◽  
Author(s):  
M Rosenkrantz ◽  
T Alam ◽  
K S Kim ◽  
B J Clark ◽  
P A Srere ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Citrate Synthase ◽  
Amino Terminus ◽  
Translation Product ◽  
Mitochondrial Targeting ◽  
E Coli ◽  
Homologous Genes ◽  
Primary Translation Product ◽  
Mitochondrial Targeting Sequences

Saccharomyces cerevisiae contains two genes, CIT1 and CIT2, encoding functional citrate synthase (K.-S. Kim, M. S. Rosenkrantz, and L. Guarente, Mol. Cell. Biol. 6:1936-1942, 1986). We show here that CIT2 encodes a nonmitochondrial form of citrate synthase. The DNA sequence of CIT2 presented provides a possible explanation for why the CIT2 product, unlike the CIT1 product, fails to be imported into mitochondria. While the products of these two genes are highly homologous, they diverge strikingly at their amino termini. The amino terminus of the CIT1 primary translation product extends 39 residues beyond the amino termini of Escherichia coli and porcine citrate synthases. This extension consists of a typical mitochondrial targeting motif. The amino terminus of the CIT2 primary translation product extends 20 residues beyond the amino termini of the E. coli and porcine enzymes. The CIT2-encoded extension is not homologous to that of CIT1, resulting in a nonmitochondrial localization of the product. The CIT2-encoded extension, however, does bear certain similarities to mitochondrial targeting sequences. The possible role of this sequence in targeting this CIT2 product to a nonmitochondrial organelle is discussed.

Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple

2018 ◽  
Vol 39 (4) ◽  
pp. 474-482
Author(s):  
Hoang Thi Le Thuong ◽  
Nguyen Quang Hao ◽  
Tran Thi Thuy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Ph ◽  
Yeast Strains ◽  
Biological Studies ◽  
Taxonomic Characterization

Eight yeast strains (denoted as D1 to D8) were isolated from samples of natural fermented pineapple. Strain D8 showed highest alcoholic production at low pH and special aroma of pineapple has been chosen for further study. Taxonomic characterization of strain D8 using morphological, biochemical and molecular biological studies confirmed that strain D8  belong to Saccharomycetaceae family, Saccharomycetales order and Saccharomyces cerevisiae species. Therefore, we named this strain as Saccharomyces cerevisiae D8 for further study on Brandy production from pineapple. Citation: Hoang Thi Le Thuong, Nguyen Quang Hao, Tran Thi Thuy, 2017. Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple. Tap chi Sinh hoc, 39(4): 474- 482. DOI: 10.15625/0866-7160/v39n4.10864.*Corresponding author: [email protected] Received 5 December 2016, accepted 12 August 2017

Characterization of sterol-ester hydrolase in Saccharomyces cerevisiae

1978 ◽  
Vol 525 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Shigeru Taketani ◽  
Takashi Osumi ◽  
Hirohiko Katsuki
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sterol Ester ◽  
Ester Hydrolase
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