scholarly journals Kinetics of Asian and African Zika Virus Lineages over Single‐cycle and Multi‐cycle Growth in Culture: gene expression, cell killing, virus production, and mathematical modeling

2021 ◽  
Author(s):  
Huicheng Shi ◽  
John Yin
Keyword(s):  
Gene Expression ◽  
Mathematical Modeling ◽  
Zika Virus ◽  
Virus Production ◽  
Cell Killing ◽  
Single Cycle ◽  
Cycle Growth ◽  
Kinetics Of

scholarly journals IP-10 and CXCR3 signaling inhibit Zika virus replication in human prostate cells

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244587
Author(s):  
Jennifer L. Spencer Clinton ◽  
Linda L. Tran ◽  
Megan B. Vogt ◽  
David R. Rowley ◽  
Jason T. Kimata ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zika Virus ◽  
Sexual Transmission ◽  
Virus Transmission ◽  
Virus Production ◽  
Viral Gene Expression ◽  
Human Prostate ◽  
Viral Gene ◽  
Prostate Cells ◽  
Cxcr3 Signaling

Our previous studies have shown that Zika virus (ZIKV) replicates in human prostate cells, suggesting that the prostate may serve as a long-term reservoir for virus transmission. Here, we demonstrated that the innate immune responses generated to three distinct ZIKV strains (all isolated from human serum) were significantly different and dependent on their passage history (in mosquito, monkey, or human cells). In addition, some of these phenotypic differences were reduced by a single additional cell culture passage, suggesting that viruses that have been passaged more than 3 times from the patient sample will no longer reflect natural phenotypes. Two of the ZIKV strains analyzed induced high levels of the IP-10 chemokine and IFNγ in human prostate epithelial and stromal mesenchymal stem cells. To further understand the importance of these innate responses on ZIKV replication, we measured the effects of IP-10 and its downstream receptor, CXCR3, on RNA and virus production in prostate cells. Treatment with IP-10, CXCR3 agonist, or CXCR3 antagonist significantly altered ZIKV viral gene expression, depending on their passage in cells of relevant hosts (mosquito or human). We detected differences in gene expression of two primary CXCR3 isoforms (CXCR3-A and CXCR3-B) on the two cell types, possibly explaining differences in viral output. Lastly, we examined the effects of IP-10, agonist, or antagonist on cell death and proliferation under physiologically relevant infection rates, and detected no significant differences. Although we did not measure protein expression directly, our results indicate that CXCR3 signaling may be a target for therapeutics, to ultimately stop sexual transmission of this virus.

scholarly journals Linking Ligand-Induced Alterations in Androgen Receptor Structure to Differential Gene Expression: A First Step in the Rational Design of Selective Androgen Receptor Modulators

2006 ◽  
Vol 20 (6) ◽  
pp. 1201-1217 ◽  
Author(s):  
Dmitri Kazmin ◽  
Tatiana Prytkova ◽  
C. Edgar Cook ◽  
Russell Wolfinger ◽  
Tzu-Ming Chu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Androgen Receptor ◽  
Rational Design ◽  
Dynamic Structure ◽  
Rational Approach ◽  
Peptide Phage Display ◽  
Differential Gene ◽  
Receptor Modulators ◽  
Kinetics Of

Abstract We have previously identified a family of novel androgen receptor (AR) ligands that, upon binding, enable AR to adopt structures distinct from that observed in the presence of canonical agonists. In this report, we describe the use of these compounds to establish a relationship between AR structure and biological activity with a view to defining a rational approach with which to identify useful selective AR modulators. To this end, we used combinatorial peptide phage display coupled with molecular dynamic structure analysis to identify the surfaces on AR that are exposed specifically in the presence of selected AR ligands. Subsequently, we used a DNA microarray analysis to demonstrate that differently conformed receptors facilitate distinct patterns of gene expression in LNCaP cells. Interestingly, we observed a complete overlap in the identity of genes expressed after treatment with mechanistically distinct AR ligands. However, it was differences in the kinetics of gene regulation that distinguished these compounds. Follow-up studies, in cell-based assays of AR action, confirmed the importance of these alterations in gene expression. Together, these studies demonstrate an important link between AR structure, gene expression, and biological outcome. This relationship provides a firm underpinning for mechanism-based screens aimed at identifying SARMs with useful clinical profiles.

scholarly journals Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender

Zygote ◽  
2011 ◽  
Vol 20 (2) ◽  
pp. 123-134 ◽  
Author(s):  
Grazieli Marinheiro Machado ◽  
Ester Siqueira Caixeta ◽  
Carolina Madeira Lucci ◽  
Rodolfo Rumpf ◽  
Maurício Machaim Franco ◽  
...  
Keyword(s):  
Gene Expression ◽  
Morphological Characteristics ◽  
Tunnel Construction ◽  
Agarose Gel ◽  
Male And Female ◽  
Embryo Size ◽  
And Gender ◽  
Phosphate Buffered Saline ◽  
Kinetics Of

SummaryThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression of male and female IVP embryos that were cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in the MQW (42%) gel. However, embryos produced in MQW were bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW (65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not significantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use of MQW was the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression.

Mathematical modeling of hot-air drying kinetics of red bell pepper (var. Lamuyo)

2007 ◽  
Vol 79 (4) ◽  
pp. 1460-1466 ◽  
Author(s):  
A. Vega ◽  
P. Fito ◽  
A. Andrés ◽  
R. Lemus
Keyword(s):  
Mathematical Modeling ◽  
Drying Kinetics ◽  
Bell Pepper ◽  
Hot Air Drying ◽  
Air Drying ◽  
Hot Air ◽  
Kinetics Of

Kinetics of antigen-specific IgM/IgG/IgA antibody responses during Zika virus natural infection in two patients

2018 ◽  
Vol 91 (5) ◽  
pp. 872-876 ◽  
Author(s):  
Ling-Zhai Zhao ◽  
Wen-Xin Hong ◽  
Jian Wang ◽  
Lei Yu ◽  
Feng-Yu Hu ◽  
...  
Keyword(s):  
Zika Virus ◽  
Natural Infection ◽  
Antibody Responses ◽  
Iga Antibody ◽  
Kinetics Of

Mathematical Modeling of Controlled-Release Kinetics of Herbicides in a Dynamic-Water-Bath System

2001 ◽  
pp. 563-574
Author(s):  
Félix M. Pereira ◽  
Adilson R. Gonçalves ◽  
André Ferraz ◽  
Flávio T. Silva ◽  
Samuel C. Oliveira
Keyword(s):  
Mathematical Modeling ◽  
Controlled Release ◽  
Release Kinetics ◽  
Water Bath ◽  
Kinetics Of
Sign in / Sign up

Export Citation Format

Share Document