Integration of metabolic pathway manipulation and promoter engineering for the fine‐tuned biosynthesis of malic acid in Bacillus coagulans

2021 ◽  
Author(s):  
Wenhui Sun ◽  
Bo Jiang ◽  
Dongying Zhao ◽  
Zhongji Pu ◽  
Yongming Bao
Keyword(s):  
Metabolic Pathway ◽  
Malic Acid ◽  
Bacillus Coagulans ◽  
Promoter Engineering

Enabling the biosynthesis of malic acid in Lactococcus lactis by establishing the reductive TCA pathway and promoter engineering

2020 ◽  
Vol 161 ◽  
pp. 107645
Author(s):  
Wenhui Sun ◽  
Bo Jiang ◽  
Yue Zhang ◽  
Jianli Guo ◽  
Dongying Zhao ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Malic Acid ◽  
Promoter Engineering

scholarly journals Discovery and Characterization of Human-Urine Utilization by Asymptomatic-Bacteriuria-Causing Streptococcus agalactiae

2015 ◽  
Vol 84 (1) ◽  
pp. 307-319 ◽  
Author(s):  
Deepak S. Ipe ◽  
Nouri L. Ben Zakour ◽  
Matthew J. Sullivan ◽  
Scott A. Beatson ◽  
Kimberly B. Ulett ◽  
...  
Keyword(s):  
Metabolic Pathway ◽  
Human Urine ◽  
Malic Acid ◽  
Streptococcus Agalactiae ◽  
Malic Enzyme ◽  
Asymptomatic Bacteriuria ◽  
Acute Cystitis ◽  
Content Type ◽  
Normal Human

Streptococcus agalactiaecauses both symptomatic cystitis and asymptomatic bacteriuria (ABU); however, growth characteristics ofS. agalactiaein human urine have not previously been reported. Here, we describe a phenotype of robust growth in human urine observed in ABU-causingS. agalactiae(ABSA) that was not seen among uropathogenicS. agalactiae(UPSA) strains isolated from patients with acute cystitis. In direct competition assays using pooled human urine inoculated with equal numbers of a prototype ABSA strain, designated ABSA 1014, and any one of several UPSA strains, measurement of the percentage of each strain recovered over time showed a markedly superior fitness of ABSA 1014 for urine growth. Comparative phenotype profiling of ABSA 1014 and UPSA strain 807, isolated from a patient with acute cystitis, using metabolic arrays of >2,500 substrates and conditions revealed unique and specificl-malic acid catabolism in ABSA 1014 that was absent in UPSA 807. Whole-genome sequencing also revealed divergence in malic enzyme-encoding genes between the strains predicted to impact the activity of the malate metabolic pathway. Comparative growth assays in urine comparing wild-type ABSA and gene-deficient mutants that were functionally inactivated for the malic enzyme metabolic pathway by targeted disruption of themaeEormaeKgene in ABSA demonstrated attenuated growth of the mutants in normal human urine as well as synthetic human urine containing malic acid. We conclude that someS. agalactiaestrains can grow in human urine, and this relates in part to malic acid metabolism, which may affect the persistence or progression ofS. agalactiaeABU.

Enrichment of common carp (Cyprinus carpio) diet with Malic acid: Effects on skin mucosal immunity, antioxidant defecne and growth performance

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Malic Acid ◽  
Control Group ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Treatment Comparison ◽  
Genes Expression ◽  
Significant Difference ◽  
And Control

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.

PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Mahyudin Abdul Rachman
Keyword(s):  
Metabolic Pathway ◽  
Acid Production ◽  
Double Mutant ◽  
Enterobacter Aerogenes ◽  
H2 Production ◽  
Hydrogen Gas ◽  
Survival Ratio ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Ethyl Methane

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12, 13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase deffi cient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methane sludge

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