scholarly journals Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology

2021 ◽  
Author(s):  
N. Romanova ◽  
T. Niemann ◽  
J.FW Greiner ◽  
B. Kaltschmidt ◽  
C. Kaltschmidt ◽  
...  
Keyword(s):  
Cellular Behavior ◽  
Culture Effects

scholarly journals Hyperosmolality in CHO Culture: Effects on cellular behavior and morphology

2020 ◽  
Author(s):  
Nadiya Romanova ◽  
Tarek Niemann ◽  
Johannes Greiner ◽  
Barbara Kaltschmidt ◽  
Christian Kaltschmidt ◽  
...  
Keyword(s):  
Cellular Behavior ◽  
Culture Effects

Use of Flexible Materials with a Novel Pressure Driven Equibiaxial Cell Stretching Device for Mechanical Stimulation of Single Mammalian Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
James D. Kubicek ◽  
Stephanie Brelsford ◽  
Philip R. LeDuc
Keyword(s):  
Cell Fate ◽  
Mechanical Stimulation ◽  
Mammalian Cells ◽  
Cellular Response ◽  
Single Cells ◽  
Complex Nature ◽  
Cellular Behavior ◽  
Cell Stretching ◽  
Stimulation Of ◽  
Pressure Driven

AbstractMechanical stimulation of single cells has been shown to affect cellular behavior from the molecular scale to ultimate cell fate including apoptosis and proliferation. In this, the ability to control the spatiotemporal application of force on cells through their extracellular matrix connections is critical to understand the cellular response of mechanotransduction. Here, we develop and utilize a novel pressure-driven equibiaxial cell stretching device (PECS) combined with an elastomeric material to control specifically the mechanical stimulation on single cells. Cells were cultured on silicone membranes coated with molecular matrices and then a uniform pressure was introduced to the opposite surface of the membrane to stretch single cells equibiaxially. This allowed us to apply mechanical deformation to investigate the complex nature of cell shape and structure. These results will enhance our knowledge of cellular and molecular function as well as provide insights into fields including biomechanics, tissue engineering, and drug discovery.

A Photoactivatable α5β1-Specific Integrin Ligand

2018 ◽  
Author(s):  
Roshna Vakkeel ◽  
Aleeza Farrukh ◽  
Aranzazu del Campo
Keyword(s):  
Cellular Response ◽  
Light Exposure ◽  
Matrix Composition ◽  
Cellular Behavior ◽  
Dynamic Variations ◽  
In Situ Activation ◽  
Controlled Exposure ◽  
Cellular Behaviour ◽  
Integrin Ligand

In order to study how dynamic changes of α5β1 integrin engagement affect cellular behaviour, photoactivatable derivatives of α5β1 specific ligands are presented in this article. The presence of the photoremovable protecting group (PRPG) introduced at a relevant position for integrin recognition, temporally inhibits ligand bioactivity. Light exposure at cell-compatible dose efficiently cleaves the PRPG and restores functionality. Selective cell response (attachment, spreading, migration) to the activated ligand on the surface is achieved upon controlled exposure. Spatial and temporal control of the cellular response is demonstrated, including the possibility to in situ activation. Photoactivatable integrin-selective ligands in model microenvironments will allow the study of cellular behavior in response to changes in the activation of individual integrins as consequence of dynamic variations of matrix composition.

Influence of MWNTs/PMMA Thin Film on Cellular Behavior of Cardiac Stem Cells From Brown Adipose

2013 ◽  
Vol 40 (10) ◽  
pp. 1070
Author(s):  
Cui-Mi DUAN ◽  
Hong-Yu SUN ◽  
Ye YUAN ◽  
Zhi-Qiang LIU ◽  
Rong-Yu TANG ◽  
...  
Keyword(s):  
Thin Film ◽  
Stem Cells ◽  
Cardiac Stem Cells ◽  
Cellular Behavior ◽  
Brown Adipose

Influence of Supramolecular Chiral Hydrogel on Cellular Behavior of Endothelial Cells Under High-Glucose-Induced Injury

2021 ◽  
Vol 26 (1) ◽  
pp. 17-24
Author(s):  
Weijie Cai ◽  
Musha Hamushan ◽  
Changli Zhao ◽  
Pengfei Cheng ◽  
Wanrun Zhong ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
High Glucose ◽  
Cellular Behavior

scholarly journals Influence of Subcellular Localization and Functional State on Protein Turnover

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1747
Author(s):  
Roya Yousefi ◽  
Kristina Jevdokimenko ◽  
Verena Kluever ◽  
David Pacheu-Grau ◽  
Eugenio F. Fornasiero
Keyword(s):  
Subcellular Localization ◽  
Functional State ◽  
Protein Turnover ◽  
Protein Localization ◽  
Small Gtpase ◽  
Turnover Rates ◽  
Cellular Behavior ◽  
Two Factors ◽  
Brain Creatine Kinase ◽  
Selection Of

Protein homeostasis is an equilibrium of paramount importance that maintains cellular performance by preserving an efficient proteome. This equilibrium avoids the accumulation of potentially toxic proteins, which could lead to cellular stress and death. While the regulators of proteostasis are the machineries controlling protein production, folding and degradation, several other factors can influence this process. Here, we have considered two factors influencing protein turnover: the subcellular localization of a protein and its functional state. For this purpose, we used an imaging approach based on the pulse-labeling of 17 representative SNAP-tag constructs for measuring protein lifetimes. With this approach, we obtained precise measurements of protein turnover rates in several subcellular compartments. We also tested a selection of mutants modulating the function of three extensively studied proteins, the Ca2+ sensor calmodulin, the small GTPase Rab5a and the brain creatine kinase (CKB). Finally, we followed up on the increased lifetime observed for the constitutively active Rab5a (Q79L), and we found that its stabilization correlates with enlarged endosomes and increased interaction with membranes. Overall, our data reveal that both changes in protein localization and functional state are key modulators of protein turnover, and protein lifetime fluctuations can be considered to infer changes in cellular behavior.

The effect of scaffold architecture on cellular behavior in vitro

2020 ◽  
Author(s):  
V. Elagin ◽  
S. Rodimova ◽  
N. Minaev ◽  
A. Shpichka ◽  
M. Karabut ◽  
...  
Keyword(s):  
Cellular Behavior ◽  
Scaffold Architecture

scholarly journals The cell biology of regeneration

2012 ◽  
Vol 196 (5) ◽  
pp. 553-562 ◽  
Author(s):  
Ryan S. King ◽  
Phillip A. Newmark
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Cell Biology ◽  
Progenitor Cell ◽  
Complex Structures ◽  
Regenerative Processes ◽  
Cellular Behavior ◽  
Positional Identity ◽  
Progenitor Cell Proliferation ◽  
Functional Analyses

Regeneration of complex structures after injury requires dramatic changes in cellular behavior. Regenerating tissues initiate a program that includes diverse processes such as wound healing, cell death, dedifferentiation, and stem (or progenitor) cell proliferation; furthermore, newly regenerated tissues must integrate polarity and positional identity cues with preexisting body structures. Gene knockdown approaches and transgenesis-based lineage and functional analyses have been instrumental in deciphering various aspects of regenerative processes in diverse animal models for studying regeneration.

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