Virus‐like particle preparation is improved by control over capsomere‐DNA interactions during chromatographic purification

2021 ◽  
Author(s):  
Lukas Gerstweiler ◽  
Jingxiu Bi ◽  
Anton Peter Jacob Middelberg
Keyword(s):  
Dna Interactions ◽  
Chromatographic Purification ◽  
Virus Like Particle ◽  
Particle Preparation

scholarly journals Poly-γ-Glutamic Acid Nanoparticles and Aluminum Adjuvant Used as an Adjuvant with a Single Dose of Japanese Encephalitis Virus-Like Particles Provide Effective Protection from Japanese Encephalitis Virus

2011 ◽  
Vol 19 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Shigefumi Okamoto ◽  
Hironori Yoshii ◽  
Masaaki Matsuura ◽  
Asato Kojima ◽  
Toyokazu Ishikawa ◽  
...  
Keyword(s):  
Single Dose ◽  
Glutamic Acid ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Single Injection ◽  
Neutralizing Antibody ◽  
Encephalitis Virus ◽  
Virus Like Particle ◽  
Generation Vaccine ◽  
Particle Preparation

ABSTRACTTo maintain immunity against Japanese encephalitis virus (JEV), a formalin-inactivated Japanese encephalitis (JE) vaccine should be administered several times. The repeated vaccination is not helpful in the case of a sudden outbreak of JEV or when urgent travel to a high-JEV-risk region is required; however, there are few single-injection JE vaccine options. In the present study, we investigated the efficacy of a single dose of a new effective JE virus-like particle preparation containing the JE envelope protein (JE-VLP). Although single administration with JE-VLP protected less than 50% of mice against lethal JEV infection, adding poly(γ-glutamic acid) nanoparticles (γ-PGA-NPs) or aluminum adjuvant (alum) to JE-VLP significantly protected more than 90% of the mice. A single injection of JE-VLP with either γ-PGA-NPs or alum induced a significantly greater anti-JEV neutralizing antibody titer than JE-VLP alone. The enhanced titers were maintained for more than 6 months, resulting in long-lasting protection of 90% of the immunized mice. Although the vaccine design needs further modification to reach 100% protection, a single dose of JE-VLP with γ-PGA-NPs may be a useful step in developing a next-generation vaccine to stop a JE outbreak or to immunize travelers or military personnel.

scholarly journals Two new double-stranded RNA molecules showing non-mendelian inheritance and heat inducibility in Saccharomyces cerevisiae

1984 ◽  
Vol 4 (1) ◽  
pp. 181-187
Author(s):  
M Wesolowski ◽  
R B Wickner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mendelian Inheritance ◽  
Chromosomal Dna ◽  
Double Stranded Rna ◽  
Rna Molecules ◽  
Genetic Elements ◽  
Virus Like Particle ◽  
Particle Preparation ◽  
Mendelian Genetic ◽  
Efficient Transmission

Certain strains of Saccharomyces cerevisiae were found to have a complex nuclear defect (designated clo-) that makes cells unable to maintain some L-B and some L-C double-stranded RNAs at 25 degrees C. The clo- strains were not defective in maintenance of L-A, M1, or M2 double-stranded RNAs. Most clo-strains lacking L and M carry small amounts of two double-stranded RNA species intermediate in size between L and M and denoted T (2.7 kilobase pairs) and W (2.25 kilobase pairs). Some strains carry both T and W, some carry neither, and some carry only W; no strains carrying only T have been found. Both T and W show 4+:0 segregation in meiosis and efficient transmission by cytoplasmic mixing (cytoduction), indicating that they are non-Mendelian genetic elements. T and W do not cross-hybridize with each other or with L-A, L-B, L-C, M1, M2, or chromosomal DNA. T and W are apparently distinct from other known non-Mendelian genetic elements (2mu DNA, [rho], [psi], 20S RNA, [URE3]). In most strains the copy number of both T and W is increased about 10-fold by the growth of cells at 37 degrees C. This heat inducibility of T and W is under control of a cytoplasmic gene. T and W double-stranded RNAs are not found in a purified L-containing virus-like particle preparation from a strain containing L-B, T, and W double-stranded RNAs. The role, if any, of T or W in the killer systems is not known.

scholarly journals Two new double-stranded RNA molecules showing non-mendelian inheritance and heat inducibility in Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (1) ◽  
pp. 181-187 ◽  
Author(s):  
M Wesolowski ◽  
R B Wickner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mendelian Inheritance ◽  
Chromosomal Dna ◽  
Double Stranded Rna ◽  
Rna Molecules ◽  
Genetic Elements ◽  
Virus Like Particle ◽  
Particle Preparation ◽  
Mendelian Genetic ◽  
Efficient Transmission

Certain strains of Saccharomyces cerevisiae were found to have a complex nuclear defect (designated clo-) that makes cells unable to maintain some L-B and some L-C double-stranded RNAs at 25 degrees C. The clo- strains were not defective in maintenance of L-A, M1, or M2 double-stranded RNAs. Most clo-strains lacking L and M carry small amounts of two double-stranded RNA species intermediate in size between L and M and denoted T (2.7 kilobase pairs) and W (2.25 kilobase pairs). Some strains carry both T and W, some carry neither, and some carry only W; no strains carrying only T have been found. Both T and W show 4+:0 segregation in meiosis and efficient transmission by cytoplasmic mixing (cytoduction), indicating that they are non-Mendelian genetic elements. T and W do not cross-hybridize with each other or with L-A, L-B, L-C, M1, M2, or chromosomal DNA. T and W are apparently distinct from other known non-Mendelian genetic elements (2mu DNA, [rho], [psi], 20S RNA, [URE3]). In most strains the copy number of both T and W is increased about 10-fold by the growth of cells at 37 degrees C. This heat inducibility of T and W is under control of a cytoplasmic gene. T and W double-stranded RNAs are not found in a purified L-containing virus-like particle preparation from a strain containing L-B, T, and W double-stranded RNAs. The role, if any, of T or W in the killer systems is not known.

scholarly journals One-shot vaccination with an insect cell-derived low-dose influenza A H7 virus-like particle preparation protects mice against H7N9 challenge

Vaccine ◽  
2014 ◽  
Vol 32 (3) ◽  
pp. 355-362 ◽  
Author(s):  
Miriam Klausberger ◽  
Monika Wilde ◽  
Dieter Palmberger ◽  
Rong Hai ◽  
Randy A. Albrecht ◽  
...  
Keyword(s):  
Insect Cell ◽  
Influenza A ◽  
Low Dose ◽  
Virus Like Particle ◽  
Particle Preparation

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Hepatitis A Antigen in Liver, Bile and Stool

1975 ◽  
Vol 33 ◽  
pp. 340-341
Author(s):  
D.R. Jackson ◽  
J.H. Hoofnagle ◽  
A.N. Schulman ◽  
J.L. Dienstag ◽  
R.H. Purcell ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Liver Enzymes ◽  
Hepatitis A Virus ◽  
Type A ◽  
Initial Study ◽  
Virus Like Particle ◽  
Elevated Liver Enzymes ◽  
Ag Particles ◽  
Ag Particle ◽  
Human Stool

Using immune electron microscopy Feinstone et. al. demonstrated the presence of a 27 nm virus-like particle in acute-phase stools of patients with viral hepatitis, type A, These hepatitis A antigen (HA Ag) particles were aggregated by convalescent serum from patients with type A hepatitis but not by pre-infection serum. Subsequently Dienstag et. al. and Maynard et. al. produced acute hepatitis in chimpanzees by inoculation with human stool containing HA Ag. During the early acute disease, virus like particles antigenically, morphologically and biophysically identical to the human HA Ag particle were found in chimpanzee stool. Recently Hilleman et. al. have described similar particles in liver and serum of marmosets infected with hepatitis A virus (HAV). We have investigated liver, bile and stool from chimpanzees and marmosets experimentally infected with HAV. In an initial study, a chimpanzee (no.785) inoculated with HA Ag-containing stool developed elevated liver enzymes 21 days after exposure.

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