scholarly journals Creation and Filtering of a Recurrent Spectral Library of CHO Cell Metabolites and Media Components

2021 ◽  
Author(s):  
Kelly H. Telu ◽  
Ramesh Marupaka ◽  
Nirina R. Andriamaharavo ◽  
Yamil Simón‐Manso ◽  
Yuxue Liang ◽  
...  
Keyword(s):  
Spectral Library ◽  
Cho Cell

Creation of Soil Spectral Library for Marathwada Region

2016 ◽  
Vol 5 (1) ◽  
pp. 1787-1794
Author(s):  
Ramdas D. Gore ◽  
◽  
Reena H. Chaudhari ◽  
Bharti W. Gawali ◽  
Keyword(s):  
Spectral Library

USGS Digital Spectral Library splib06a

Data Series ◽  
10.3133/ds231 ◽  
2007 ◽  
Author(s):  
Roger N. Clark ◽  
Gregg A. Swayze ◽  
Richard A. Wise ◽  
K. Eric Livo ◽  
Todd M. Hoefen ◽  
...  
Keyword(s):  
Spectral Library

Separation and Detection of Caffeine, Theophylline and Theobromine from Coffee Varieties, Carbonated Soft Drinks and Alcoholic Beverages

2017 ◽  
Vol 68 (11) ◽  
pp. 2704-2707
Author(s):  
Delia Nica Badea ◽  
Codrina Levai
Keyword(s):  
Food Safety ◽  
Mobile Phase ◽  
Soft Drinks ◽  
Alcoholic Beverages ◽  
Instrumental Method ◽  
Spectral Library ◽  
Active Components ◽  
Carbonated Soft Drinks ◽  
Mass Detector ◽  
Methyl Xanthine

The paper evaluates the presence of methyl xanthine compounds: caffeine, theophylline, theobromine used as ingredients in carbonated soft drinks or as color and flavor ingredients in alcoholic beverages. The active components extracted from the selected products (coffee, tea, drinks) was separated and identified chromatographically using plates with silica nano -Sil NH2 / UV-254, mobile phase ethanol - water (50: 1, 50: 3, 50: 5; 50: 7; v / v) and 60 F254 plates, mobile phase acetone-toluene-chloroform (40:30:30 v / v). Separated caffeine and identified by TLC was analyzed using a HelWet Packard 5890 Gas Chromatograph equipped with MS 5972 mass detector and spectral library to confirm identification. This simple and rapid TLC, GC / MS instrumental method is useful in controlling traces of methyl xanthine compounds in food as a food safety measure.is useful in controlling traces compound of food products containing methylxanthines as a food safety measure.

Development and validation of a spectral library searching method for peptide identification from MS/MS

PROTEOMICS ◽  
2007 ◽  
Vol 7 (5) ◽  
pp. 655-667 ◽  
Author(s):  
Henry Lam ◽  
Eric W. Deutsch ◽  
James S. Eddes ◽  
Jimmy K. Eng ◽  
Nichole King ◽  
...  
Keyword(s):  
Peptide Identification ◽  
Spectral Library ◽  
Searching Method ◽  
Development And Validation

scholarly journals Recombinant γY278H Fibrinogen Showed Normal Secretion from CHO Cells, but a Corresponding Heterozygous Patient Showed Hypofibrinogenemia

2021 ◽  
Vol 22 (10) ◽  
pp. 5218
Author(s):  
Tomu Kamijo ◽  
Takahiro Kaido ◽  
Masahiro Yoda ◽  
Shinpei Arai ◽  
Kazuyoshi Yamauchi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cho Cells ◽  
Fibrinogen Level ◽  
Culture Media ◽  
Chinese Hamster ◽  
Plasma Fibrinogen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cho Cell ◽  
Accelerated Degradation ◽  
Heterozygous Patient

We identified a novel heterozygous hypofibrinogenemia, γY278H (Hiroshima). To demonstrate the cause of reduced plasma fibrinogen levels (functional level: 1.12 g/L and antigenic level: 1.16 g/L), we established γY278H fibrinogen-producing Chinese hamster ovary (CHO) cells. An enzyme-linked immunosorbent assay demonstrated that synthesis of γY278H fibrinogen inside CHO cells and secretion into the culture media were not reduced. Then, we established an additional five variant fibrinogen-producing CHO cell lines (γL276P, γT277P, γT277R, γA279D, and γY280C) and conducted further investigations. We have already established 33 γ-module variant fibrinogen-producing CHO cell lines, including 6 cell lines in this study, but only the γY278H and γT277R cell lines showed disagreement, namely, recombinant fibrinogen production was not reduced but the patients’ plasma fibrinogen level was reduced. Finally, we performed fibrinogen degradation assays and demonstrated that the γY278H and γT277R fibrinogens were easily cleaved by plasmin whereas their polymerization in the presence of Ca2+ and “D:D” interaction was normal. In conclusion, our investigation suggested that patient γY278H showed hypofibrinogenemia because γY278H fibrinogen was secreted normally from the patient’s hepatocytes but then underwent accelerated degradation by plasmin in the circulation.

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