Semi‐rational engineering of an amino acid racemase that is stabilized in aqueous/organic solvent mixtures

2020 ◽  
Vol 117 (9) ◽  
pp. 2683-2693 ◽  
Author(s):  
Christian Femmer ◽  
Matthias Bechtold ◽  
Sven Panke
Keyword(s):  
Amino Acid ◽  
Organic Solvent ◽  
Solvent Mixtures ◽  
Rational Engineering ◽  
Amino Acid Racemase

Characterization of a novel moderate-substrate specificity amino acid racemase from the hyperthermophilic archaeon Thermococcus litoralis

2021 ◽  
Author(s):  
Ryushi Kawakami ◽  
Chinatsu Kinoshita ◽  
Tomoki Kawase ◽  
Mikio Sato ◽  
Junji Hayashi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Substrate Specificity ◽  
Enzyme Stability ◽  
Hyperthermophilic Archaea ◽  
Thermococcus Litoralis ◽  
Hyperthermophilic Archaeon ◽  
Aminobutyrate Aminotransferase ◽  
Chaperone Protein ◽  
Amino Acid Racemase

Abstract The amino acid sequence of the OCC_10945 gene product from the hyperthermophilic archaeon Thermococcus litoralis DSM5473, originally annotated as γ-aminobutyrate aminotransferase, is highly similar to that of the uncharacterized pyridoxal 5ʹ-phosphate (PLP)-dependent amino acid racemase from Pyrococcus horikoshii. The OCC_10945 enzyme was successfully overexpressed in Escherichia coli by co-expression with a chaperone protein. The purified enzyme demonstrated PLP-dependent amino acid racemase activity primarily toward Met and Leu. Although PLP contributed to enzyme stability, it only loosely bound to this enzyme. Enzyme activity was strongly inhibited by several metal ions, including Co2+ and Zn2+, and non-substrate amino acids such as l-Arg and l-Lys. These results suggest that the underlying PLP-binding and substrate recognition mechanisms in this enzyme are significantly different from those of the other archaeal and bacterial amino acid racemases. This is the first description of a novel PLP-dependent amino acid racemase with moderate substrate specificity in hyperthermophilic archaea.

Efficient Conversion of Threonine to Allothreonine Using Immobilized Amino Acid Racemase and Temperature Cycles

2021 ◽  
Author(s):  
Kritsada Intaraboonrod ◽  
Andreas Seidel-Morgenstern ◽  
Heike Lorenz ◽  
Adrian E. Flood
Keyword(s):  
Amino Acid ◽  
Temperature Cycles ◽  
Efficient Conversion ◽  
Amino Acid Racemase

Thermodynamic properties of n-alkoxyethanols + organic solvent mixtures

2003 ◽  
Vol 403 (2) ◽  
pp. 223-229 ◽  
Author(s):  
F.J. Carmona ◽  
J.Antonio González ◽  
Isaı́as Garcı́a de la Fuente ◽  
J.Carlos Cobos
Keyword(s):  
Thermodynamic Properties ◽  
Organic Solvent ◽  
Solvent Mixtures

Ionic equilibria in aqueous organic solvent mixtures The equilibria of HF in an ethanol + water mixture used for cleaning up semiconductors

1997 ◽  
Vol 433 (1-2) ◽  
pp. 77-83 ◽  
Author(s):  
Clara Ràfols ◽  
Koit Herodes ◽  
JoséLuis Beltrán ◽  
Elisabeth Bosch ◽  
Martí Rosés
Keyword(s):  
Organic Solvent ◽  
Solvent Mixtures ◽  
Water Mixture ◽  
Ionic Equilibria

Occurrence of a unique amino acid racemase in a basidiomycetous mushroom, Lentinus edodes

2003 ◽  
Vol 23 (2-6) ◽  
pp. 379-387 ◽  
Author(s):  
Akira Watanabe ◽  
Shiro Yamaguchi ◽  
Koichiro Urabe ◽  
Yasuhiko Asada
Keyword(s):  
Amino Acid ◽  
Lentinus Edodes ◽  
Basidiomycetous Mushroom ◽  
Unique Amino Acid ◽  
Amino Acid Racemase
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