scholarly journals Enhancing plasmid transformation efficiency and enabling CRISPR‐Cas9/Cpf1‐based genome editing in Clostridium tyrobutyricum

2020 ◽  
Vol 117 (9) ◽  
pp. 2911-2917 ◽  
Author(s):  
Jie Zhang ◽  
Wei Hong ◽  
Liang Guo ◽  
Yifen Wang ◽  
Yi Wang
Keyword(s):  
Genome Editing ◽  
Transformation Efficiency ◽  
Clostridium Tyrobutyricum ◽  
Plasmid Transformation

Effect of lipopolysaccharide mutations and temperature on plasmid transformation efficiency in Pseudomonas aeruginosa

1986 ◽  
Vol 32 (5) ◽  
pp. 436-438 ◽  
Author(s):  
Denise Berry ◽  
Andrew M. Kropinski
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Growth Temperature ◽  
Type Strain ◽  
Wild Type Strain ◽  
Transformation Efficiency ◽  
Wild Type ◽  
Plasmid Transformation

We have isolated, and characterized electrophoretically, two new lipopolysaccharide-defective (rough) mutants of Pseudomonas aeruginosa strain PAO. These strains, AK1401 and AK1414, together with two previously characterized isolates, AK1012 and AK1282, were used as recipients in transformation experiments with plasmid pR01614 DNA. The roughest mutant, AK1282, was not transformable, while the transformation efficiency of AK1012, and to a lesser extent the wild-type strain, was dependent upon the growth temperature. The two new isolates which are less rough than AK1012 were transformed at a frequency equivalent to that of the wild type-strain.

Cell de-energization prevents plasmid transformation of yeast Saccharomyces cerevisiae: evidence for the requirement of ATP

2010 ◽  
Vol 5 (1) ◽  
pp. 78-82 ◽  
Author(s):  
Larisa Chaustova ◽  
Valė Miliukienė ◽  
Aurelijus Zimkus
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transformation Efficiency ◽  
Energy Requirement ◽  
Yeast Cells ◽  
Sodium Arsenate ◽  
Yeast Saccharomyces Cerevisiae ◽  
Plasmid Transformation ◽  
Substrate Level Phosphorylation ◽  
Intracellular Atp ◽  
Substrate Level

AbstractThe dependence of the yeast Saccharomyces cerevisiae transformation on energy requirement was studied. The inhibitory effect of sodium arsenate, used for the depletion of the intracellular ATP pool, was determined. Incubation of the yeast cells in 5 mM sodium arsenate diminished ATP accumulation by 50% and the transformation efficiency decreased by 65%. To discriminate between ATP produced by substrate level phosphorylation and oxidative phosphorylation, the inhibitory analysis of a mutant with defective mitochondria was performed. Sodium fluoride (10–50 mM), as inhibitor of glycolysis, elicited a concentration-dependent decrease in intracellular ATP levels in both parental and mutant cells. The equal transformation efficiency of the mitochondrial mutant and parental strain, in addition to experiments with oligomycin, demonstrated the independence of plasmid transformation on mitochondrial ATP synthesis. This is consistent with our hypothesis that yeast transformation efficiency is associated with ATP produced by substrate level phosphorylation.

scholarly journals Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

2021 ◽  
Vol 12 ◽  
Author(s):  
Sunny Ahmar ◽  
Tahir Mahmood ◽  
Sajid Fiaz ◽  
Freddy Mora-Poblete ◽  
Muhammad Sohaib Shafique ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Genome Editing ◽  
Transformation Efficiency ◽  
Crop Improvement ◽  
Agricultural Practices ◽  
Delivery Systems ◽  
Breeding Strategy ◽  
Specific Gene ◽  
Improvement Programs ◽  
Crop Enhancement

Agriculture is an important source of human food. However, current agricultural practices need modernizing and strengthening to fulfill the increasing food requirements of the growing worldwide population. Genome editing (GE) technology has been used to produce plants with improved yields and nutritional value as well as with higher resilience to herbicides, insects, and diseases. Several GE tools have been developed recently, including clustered regularly interspaced short palindromic repeats (CRISPR) with nucleases, a customizable and successful method. The main steps of the GE process involve introducing transgenes or CRISPR into plants via specific gene delivery systems. However, GE tools have certain limitations, including time-consuming and complicated protocols, potential tissue damage, DNA incorporation in the host genome, and low transformation efficiency. To overcome these issues, nanotechnology has emerged as a groundbreaking and modern technique. Nanoparticle-mediated gene delivery is superior to conventional biomolecular approaches because it enhances the transformation efficiency for both temporal (transient) and permanent (stable) genetic modifications in various plant species. However, with the discoveries of various advanced technologies, certain challenges in developing a short-term breeding strategy in plants remain. Thus, in this review, nanobased delivery systems and plant genetic engineering challenges are discussed in detail. Moreover, we have suggested an effective method to hasten crop improvement programs by combining current technologies, such as speed breeding and CRISPR/Cas, with nanotechnology. The overall aim of this review is to provide a detailed overview of nanotechnology-based CRISPR techniques for plant transformation and suggest applications for possible crop enhancement.

Genome editing: Wollen wir das wirklich?

2017 ◽  
Vol 6 (3) ◽  
pp. 162-162
Author(s):  
Liane Kaufmann ◽  
Michael von Aster
Keyword(s):  
Genome Editing

Genome editing (CRISPR-Cas9) to identify and characterise functional variants determining metformin response

2018 ◽  
Author(s):  
M Keller ◽  
J Dalla-Riva ◽  
A Kurbasic ◽  
M Al-Majdoub ◽  
P Spegel ◽  
...  
Keyword(s):  
Genome Editing ◽  
Functional Variants
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