scholarly journals A versatile oscillating‐flow microfluidic PCR system utilizing a thermal gradient for nucleic acid analysis

2020 ◽  
Vol 117 (5) ◽  
pp. 1525-1532 ◽  
Author(s):  
Varun L. Kopparthy ◽  
Niel D. Crews
Keyword(s):  
Nucleic Acid ◽  
Thermal Gradient ◽  
Oscillating Flow ◽  
Nucleic Acid Analysis

scholarly journals Oscillating-flow Thermal Gradient PCR

2019 ◽  
Author(s):  
Varun L. Kopparthy ◽  
Niel D. Crews
Keyword(s):  
Thermal Gradient ◽  
Oscillating Flow ◽  
Gradient System ◽  
Rna Amplification ◽  
Dna And Rna ◽  
Simultaneous Acquisition ◽  
Phage Dna ◽  
Nucleic Acid Analysis ◽  
Microscope Slides ◽  
Glass Microscope

ABSTRACTWe report the development of a versatile system based on oscillating-flow methodology in a thermal gradient system for nucleic acid analysis. Analysis of DNA and RNA samples were performed in the device, without additional temperature control and complexity. The technique reported in this study eliminates the need for predetermined fluidic channels for thermocycles, and complexity involved with additional incubation steps required for RNA amplification. A microfluidic device was fabricated using rapid prototyping by simply sandwiching dual side adhesive Kapton tape and a PDMS spacer between glass microscope slides. Amplification of the 181-bp segment of a viral phage DNA (ΦX174) and B2M gene in human RNA samples was demonstrated using the system. The developed system enables simultaneous acquisition of amplification and melt curves, eliminating the need for post-processing.

scholarly journals Alpha-Helical Fragaceatoxin C Nanopore Engineered for Double-Stranded and Single-Stranded Nucleic Acid Analysis

2016 ◽  
Vol 128 (40) ◽  
pp. 12682-12686 ◽  
Author(s):  
Carsten Wloka ◽  
Natalie Lisa Mutter ◽  
Misha Soskine ◽  
Giovanni Maglia
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Analysis

Fully Integrated Lab-on-a-Disc for Nucleic Acid Analysis of Food-Borne Pathogens

2014 ◽  
Vol 86 (8) ◽  
pp. 3841-3848 ◽  
Author(s):  
Tae-Hyeong Kim ◽  
Juhee Park ◽  
Chi-Ju Kim ◽  
Yoon-Kyoung Cho
Keyword(s):  
Nucleic Acid ◽  
Fully Integrated ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Nucleic Acid Analysis

Nucleic acids in prion preparations: unspecific background or essential component?

1994 ◽  
Vol 343 (1306) ◽  
pp. 425-430 ◽  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Large Scale ◽  
Size Range ◽  
Complete Recovery ◽  
Tissue Homogenate ◽  
Purification Step ◽  
Prolonged Incubation ◽  
Purification Scheme ◽  
Nucleic Acid Analysis

As recently published (Kellings et al. J. gen Vir. 73, 1025-1029 (1992)), the analysis of purified scrapie prions by return refocusing gel electrophoresis revealed remaining nucleic acids in the size range up to 1100 nucleotides. The results defined the possible characteristics of a hypothetical scrapie-specific nucleic acid. If homogeneous in size, such a molecule would be less than 80 nucleotides in length at a particle-toinfectivity ratio (p: i) near unity; if heterogeneous, scrapie-specific nucleic acids would have to include molecules smaller than 240 nucleotides. To decrease the amount of nucleic acids, several modifications of the PrP Sc purification scheme were introduced. Instead of sucrose gradient, ultrafiltration was applied as a purification step and nucleic acids were degraded by BenzonasetM after ultrafiltration, but significant reduction of the p: i ratio could not be achieved. To prevent trapping of nucleic acids in prion rods, nuclease (Benzonase™ ) was added into the tissue homogenate and incubated at 37°C, overnight. The Benzonase treatment revealed no loss of infectivity, but the whole procedure of nucleic acid analysis did not lead to a reduction of the p :i ratio. In another approach the number of nucleic acid degradations steps was reduced to essentially two steps: Zn 2+ hydrolysis and Benzonase digestion. Higher Zn 2+ concentrations and prolonged incubation times resulted in a more efficient nucleic acid degradation. The bioassays yielded complete recovery of infectivity. Large-scale preparations for determining the p: i ratio are still underway

Lateral flow devices for nucleic acid analysis exploiting quantum dots as reporters

2015 ◽  
Vol 864 ◽  
pp. 48-54 ◽  
Author(s):  
Eleni A. Sapountzi ◽  
Sotirios S. Tragoulias ◽  
Despina P. Kalogianni ◽  
Penelope C. Ioannou ◽  
Theodore K. Christopoulos
Keyword(s):  
Quantum Dots ◽  
Nucleic Acid ◽  
Lateral Flow ◽  
Nucleic Acid Analysis ◽  
Flow Devices

Use of Solid Amalgam Electrodes in Nucleic Acid Analysis

2002 ◽  
Vol 14 (21) ◽  
pp. 1488-1493 ◽  
Author(s):  
Bogdan Yosypchuk ◽  
Michael Heyrovský ◽  
Emil Palecek ◽  
Ladislav Novotný
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Analysis

Local Nucleic Acid Analysis of Adherent Cells

2018 ◽  
pp. 115-137
Author(s):  
Aditya Kashyap ◽  
Deborah Huber ◽  
Julien Autebert ◽  
Govind V. Kaigala
Keyword(s):  
Nucleic Acid ◽  
Adherent Cells ◽  
Nucleic Acid Analysis
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