Improved protein A resin for antibody capture in a continuous countercurrent tangential chromatography system

2019 ◽  
Vol 117 (3) ◽  
pp. 646-653 ◽  
Author(s):  
Dmitriy Fedorenko ◽  
Amit K. Dutta ◽  
Jasmine Tan ◽  
Jonathan Walko ◽  
Mark Brower ◽  
...  
Keyword(s):  
Protein A ◽  
Antibody Capture ◽  
Continuous Countercurrent

scholarly journals Affinity Chromatography: An Enabling Technology for Large Scale Bioprocessing - How does it get to the Developing World

2021 ◽  
Vol 2 (7) ◽  
pp. 01-02
Author(s):  
Frank Riske ◽  
Brendan Riske
Keyword(s):  
Affinity Chromatography ◽  
Large Scale ◽  
Developing World ◽  
Process Development ◽  
Enzyme Inhibitor ◽  
Specific Interaction ◽  
Protein A ◽  
Biological Interactions ◽  
Antibody Capture ◽  
Developed World

Affinity chromatography was initially used to describe chromatographic biological interactions such as lectin-glycoprotein, antibody -antigen and enzyme-inhibitor. This definition has expanded to include the specific interaction between a target and a ligand. The use of affinity chromatography has reached a zenith with the explosion of Mab therapeutics and the use of Protein-A chromatography for antibody capture. Now, affinity chromatography has moved to non Mab proteins. This can result in the same economic advantages as Mab, by enabling the standardization of process development and manufacturing processes in flexible multiproduct production sites. The output is improved product throughput, higher target recoveries, and potentially less expensive drugs. These advantages are available to the developed world but how do we make this technology available to the developing world?

Performance optimization of continuous countercurrent tangential chromatography for antibody capture

2016 ◽  
Vol 32 (2) ◽  
pp. 430-439 ◽  
Author(s):  
Amit K. Dutta ◽  
Jasmine Tan ◽  
Boris Napadensky ◽  
Andrew L. Zydney ◽  
Oleg Shinkazh
Keyword(s):  
Performance Optimization ◽  
Antibody Capture ◽  
Continuous Countercurrent

Optimization of protein A chromatography for antibody capture

2012 ◽  
pp. 1367-1371 ◽  
Author(s):  
Candy Ng ◽  
Hector Osuna-Sanchez ◽  
Eric Valéry ◽  
Daniel Bracewell ◽  
Eva Sørensen
Keyword(s):  
Protein A ◽  
Antibody Capture

scholarly journals Design of high productivity antibody capture by protein A chromatography using an integrated experimental and modeling approach

2012 ◽  
Vol 899 ◽  
pp. 116-126 ◽  
Author(s):  
Candy K.S. Ng ◽  
Hector Osuna-Sanchez ◽  
Eric Valéry ◽  
Eva Sørensen ◽  
Daniel G. Bracewell
Keyword(s):  
Protein A ◽  
High Productivity ◽  
Modeling Approach ◽  
Antibody Capture

Protein A antibody-capture ELISA (PACE): an ELISA format to avoid denaturation of surface-adsorbed antigens

1993 ◽  
Vol 158 (2) ◽  
pp. 267-276 ◽  
Author(s):  
Philip K.M. Ngai ◽  
Friederike Ackermann ◽  
Hans Wendt ◽  
Reto Savoca ◽  
Hans Rudolf Bosshard
Keyword(s):  
Protein A ◽  
Capture Elisa ◽  
Elisa Format ◽  
Antibody Capture

Use of Protein a Conjugated to Peroxidase to Localize Immunoglobulin G in SSPE Ferret Brain

1982 ◽  
Vol 40 ◽  
pp. 350-351
Author(s):  
Hannah R. Brown ◽  
Anthony F. Nostro ◽  
Halldor Thormar
Keyword(s):  
Immunoglobulin G ◽  
Human Disease ◽  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Inflammatory Lesions ◽  
Sspe Virus ◽  
Specific Immunoglobulin ◽  
Antibody Titers ◽  
The Brain

Subacute sclerosing panencephalitis (SSPE) is a slowly progressing disease of the CNS in children which is caused by measles virus. Ferrets immunized with measles virus prior to inoculation with the cell associated, syncytiogenic D.R. strain of SSPE virus exhibit characteristics very similar to the human disease. Measles virus nucleocapsids are present, high measles antibody titers are found in the sera and inflammatory lesions are prominent in the brains. Measles virus specific immunoglobulin G (IgG) is present in the brain,and IgG/ albumin ratios indicate that the antibodies are synthesized within the CNS.

Presence of antibody in virus-infected brain cells of SSPE ferrets

1983 ◽  
Vol 41 ◽  
pp. 804-805
Author(s):  
Hannah R. Brown ◽  
Tammy L. Donato ◽  
Halldor Thormar
Keyword(s):  
Measles Virus ◽  
Plasma Cells ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Neutralizing Antibody ◽  
Brain Cells ◽  
Antibody Titers ◽  
A Cell ◽  
The Central Nervous System ◽  
The Brain

Measles virus specific immunoglobulin G (IgG) has been found in the brains of patients with subacute sclerosing panencephalitis (SSPE), a slowly progressing disease of the central nervous system (CNS) in children. IgG/albumin ratios indicate that the antibodies are synthesized within the CNS. Using the ferret as an animal model to study the disease, we have been attempting to localize the Ig's in the brains of animals inoculated with a cell associated strain of SSPE. In an earlier report, preliminary results using Protein A conjugated to horseradish peroxidase (PrAPx) (Dynatech Diagnostics Inc., South Windham, ME.) to detect antibodies revealed the presence of immunoglobulin mainly in antibody-producing plasma cells in inflammatory lesions and not in infected brain cells.In the present experiment we studied the brain of an SSPE ferret with neutralizing antibody titers of 1:1024 in serum and 1:512 in CSF at time of sacrifice 7 months after i.c. inoculation with SSPE measles virus-infected cells. The animal was perfused with saline and portions of the brain and spinal cord were immersed in periodate-lysine-paraformaldehyde (P-L-P) fixative. The ferret was not perfused with fixative because parts of the brain were used for virus isolation.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

Role of PKC isozymes in water transport in toad urinary bladder

1991 ◽  
Vol 49 ◽  
pp. 302-303
Author(s):  
A.J. Mia ◽  
L.X. Oakford ◽  
T. Yorio
Keyword(s):  
Urinary Bladder ◽  
Apical Membrane ◽  
Membrane Surface ◽  
Protein A ◽  
Cell Types ◽  
Leukemic Cells ◽  
Toad Urinary Bladder ◽  
Pkc Isozymes ◽  
Antibody Labeling

Protein kinase C (PKC) isozymes, when activated, are translocated to particulate membrane fractions for transport to the apical membrane surface in a variety of cell types. Evidence of PKC translocation was demonstrated in human megakaryoblastic leukemic cells, and in cardiac myocytes and fibroblasts, using FTTC immunofluorescent antibody labeling techniques. Recently, we reported immunogold localizations of PKC subtypes I and II in toad urinary bladder epithelia, following 60 min stimulation with Mezerein (MZ), a PKC activator, or antidiuretic hormone (ADH). Localization of isozyme subtypes I and n was carried out in separate grids using specific monoclonal antibodies with subsequent labeling with 20nm protein A-gold probes. Each PKC subtype was found to be distributed singularly and in discrete isolated patches in the cytosol as well as in the apical membrane domains. To determine if the PKC isozymes co-localized within the cell, a double immunogold labeling technique using single grids was utilized.

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