In vitro lung cancer multicellular tumor spheroid formation using a microfluidic device

2019 ◽  
Vol 116 (11) ◽  
pp. 3041-3052 ◽  
Author(s):  
Sang Woo Lee ◽  
Soyoung Hong ◽  
Boyoung Jung ◽  
Soo Yeon Jeong ◽  
Jae Hee Byeon ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Microfluidic Device ◽  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Multicellular Tumor Spheroid

scholarly journals Mimicking Tumor Hypoxia in Non-Small Cell Lung Cancer Employing Three-Dimensional In Vitro Models

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 141
Author(s):  
Iwona Ziółkowska-Suchanek
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Tumor Hypoxia ◽  
Three Dimensional ◽  
In Vitro Models ◽  
Small Cell ◽  
Small Cell Lung ◽  
Multicellular Tumor Spheroid

Hypoxia is the most common microenvironment feature of lung cancer tumors, which affects cancer progression, metastasis and metabolism. Oxygen induces both proteomic and genomic changes within tumor cells, which cause many alternations in the tumor microenvironment (TME). This review defines current knowledge in the field of tumor hypoxia in non-small cell lung cancer (NSCLC), including biology, biomarkers, in vitro and in vivo studies and also hypoxia imaging and detection. While classic two-dimensional (2D) in vitro research models reveal some hypoxia dependent manifestations, three-dimensional (3D) cell culture models more accurately replicate the hypoxic TME. In this study, a systematic review of the current NSCLC 3D models that have been able to mimic the hypoxic TME is presented. The multicellular tumor spheroid, organoids, scaffolds, microfluidic devices and 3D bioprinting currently being utilized in NSCLC hypoxia studies are reviewed. Additionally, the utilization of 3D in vitro models for exploring biological and therapeutic parameters in the future is described.

scholarly journals Multicellular tumor spheroid formation in duplex microcapsules for analysis of chemosensitivity

2012 ◽  
Vol 103 (3) ◽  
pp. 549-554 ◽  
Author(s):  
Shinji Sakai ◽  
Kazuya Inamoto ◽  
Yang Liu ◽  
Shinji Tanaka ◽  
Shigeki Arii ◽  
...  
Keyword(s):  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Multicellular Tumor Spheroid

A multicellular tumor spheroid formation and extraction chip

2010 ◽  
Author(s):  
Hye-Jin Jin ◽  
Taeyoon Kim ◽  
Young-Ho Cho ◽  
Jin-Mo Gu ◽  
Jhingook Kim ◽  
...  
Keyword(s):  
Tumor Spheroid ◽  
Spheroid Formation ◽  
Multicellular Tumor Spheroid

Development of an in Vitro Multicellular Tumor Spheroid Model Using Microencapsulation and Its Application in Anticancer Drug Screening and Testing

2008 ◽  
Vol 21 (4) ◽  
pp. 1289-1296 ◽  
Author(s):  
Xulang Zhang ◽  
Wei Wang ◽  
Weiting Yu ◽  
Yubing Xie ◽  
Xiaohui Zhang ◽  
...  
Keyword(s):  
Anticancer Drug ◽  
Drug Screening ◽  
Tumor Spheroid ◽  
Multicellular Tumor Spheroid

In Vitro Chemosensitivity Testing Using the Multicellular Tumor Spheroid Model

1987 ◽  
Vol 4 (2) ◽  
pp. 63-73 ◽  
Author(s):  
D.J. KERR ◽  
T.E. WHELDON ◽  
A.M. KERR ◽  
S.B. KAYE
Keyword(s):  
Tumor Spheroid ◽  
Multicellular Tumor Spheroid ◽  
Chemosensitivity Testing ◽  
In Vitro Chemosensitivity

scholarly journals Detachment-induced E-cadherin expression promotes 3D tumor spheroid formation but inhibits tumor formation and metastasis of lung cancer cells

2017 ◽  
Vol 313 (5) ◽  
pp. C556-C566 ◽  
Author(s):  
Phattrakorn Powan ◽  
Sudjit Luanpitpong ◽  
Xiaoqing He ◽  
Yon Rojanasakul ◽  
Pithi Chanvorachote
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Epithelial To Mesenchymal Transition ◽  
Tumor Formation ◽  
Lung Cancer Cells ◽  
Tumor Spheroid ◽  
Mesenchymal Transition ◽  
E Cadherin

The epithelial-to-mesenchymal transition is proposed to be a key mechanism responsible for metastasis-related deaths. Similarly, cancer stem cells (CSCs) have been proposed to be a key driver of tumor metastasis. However, the link between the two events and their control mechanisms is unclear. We used a three-dimensional (3D) tumor spheroid assay and other CSC-indicating assays to investigate the role of E-cadherin in CSC regulation and its association to epithelial-to-mesenchymal transition in lung cancer cells. Ectopic overexpression and knockdown of E-cadherin were found to promote and retard, respectively, the formation of tumor spheroids in vitro but had opposite effects on tumor formation and metastasis in vivo in a xenograft mouse model. We explored the discrepancy between the in vitro and in vivo results and demonstrated, for the first time, that E-cadherin is required as a component of a major survival pathway under detachment conditions. Downregulation of E-cadherin increased the stemness of lung cancer cells but had an adverse effect on their survival, particularly on non-CSCs. Such downregulation also promoted anoikis resistance and invasiveness of lung cancer cells. These results suggest that anoikis assay could be used as an alternative method for in vitro assessment of CSCs that involves dysregulated adhesion proteins. Our data also suggest that agents that restore E-cadherin expression may be used as therapeutic agents for metastatic cancers.

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