Control of antibody high and low molecular weight species by depth filtration‐based cell culture harvesting

2019 ◽  
Vol 116 (10) ◽  
pp. 2610-2620 ◽  
Author(s):  
Deqiang Yu ◽  
Mukesh Mayani ◽  
Yuanli Song ◽  
Zhizhuo Xing ◽  
Sanchayita Ghose ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cell Culture ◽  
Low Molecular Weight ◽  
Depth Filtration

Differential effects of heparin and low molecular weight heparin on osteoblastogenesis and adipogenesis in vitro

2004 ◽  
Vol 92 (10) ◽  
pp. 803-810 ◽  
Author(s):  
Stephanie Osip ◽  
Martin Butcher ◽  
Edward Young ◽  
Lufang Yang ◽  
Stephen Shaughnessy
Keyword(s):  
Molecular Weight ◽  
Cell Culture ◽  
Bone Loss ◽  
Culture System ◽  
Low Molecular Weight Heparin ◽  
Low Molecular Weight ◽  
Cell Culture System ◽  
Term Administration ◽  
Stromal Cell Culture

SummaryWe have previously demonstrated that heparin produces cancellous bone loss in rats due in part to a decrease in the number of osteoblasts lining the trabecular bone surface. In the present study, we use a stromal-derived cell culture system together with measurements of alkaline phosphatase (ALP) activity, to compare the effects of heparin and the low molecular weight heparin (LMWH), Fragmin, on osteoblast differentiation in vitro. In addition, we examined the possibility that both heparin and LMWH can induce adipogenesis in our stromal cell culture system. Both heparin and LMWH were found to produce a statistically significant (P <0.01) and concentration-dependent decrease in the number of osteoblasts while increasing the number of adipocytes. When the effects of gravimetrically equivalent amounts of heparin and LMWH were compared, heparin had a 4-fold greater effect than LMWH. In contrast to heparin, N-desulfated heparin was found to have minimal effects on both osteoblast and adipocyte differentiation indicating that the heparin effect is not only chain-length dependent but also charge-dependent. The observation that LMWH has less of an effect on bone formation than heparin is compatible with the results of clinical trials indicating that LMWH produces less bone loss after long-term administration.

P.1.019 Neuroprotective properties of a low molecular weight dipeptide analogue of BDNF -GSB-106 in cell culture

2011 ◽  
Vol 21 ◽  
pp. S126-S127
Author(s):  
I.O. Logvinov ◽  
T.A. Antipova ◽  
A.T. Tarasiuk ◽  
T.A. Gudasheva
Keyword(s):  
Molecular Weight ◽  
Cell Culture ◽  
Low Molecular Weight

scholarly journals LMW Heparin Prevents Increased Kidney Expression of Proinflammatory Mediators in (NZBxNZW)F1 Mice

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Annica Hedberg ◽  
Premasany Kanapathippillai ◽  
Ole Petter Rekvig ◽  
Kristin Andreassen Fenton
Keyword(s):  
Molecular Weight ◽  
Cell Culture ◽  
Cell Proliferation ◽  
Cell Activation ◽  
Thymidine Incorporation ◽  
Low Molecular Weight ◽  
Activation Markers ◽  
Autoantibody Production ◽  
Proinflammatory Mediators

We have previously demonstrated that continuous infusion of low molecular weight (LMW) heparin delays autoantibody production and development of lupus nephritis in (NZBxNZW)F1 (B/W) mice. In this study we investigated the effect of LMW heparin on renal cytokine and chemokine expression and on nucleosome-mediated activation of nucleosome-specific splenocytes. Total mRNA extracted from kidneys of heparin-treated or -untreated B/W mice was analysed by qPCR for the expression of several cytokines, chemokines, and Toll-like receptors. Splenocytes taken from B/W mice were stimulated with nucleosomes with or without the presence of heparin. Splenocyte cell proliferation as thymidine incorporation and the expression of costimulatory molecules and cell activation markers were measured. Heparin treatment of B/W mice reduced thein vivoexpression of CCR2, IL1β, and TLR7 compared to untreated B/W mice. Nucleosome-induced cell proliferation of splenocytes was not influenced by heparin. The expression of CD80, CD86, CD69, CD25, CTLA-4, and TLR 2, 7, 8, and 9 was upregulated upon stimulation by nucleosomes, irrespective of whether heparin was added to the cell culture or not. In conclusion, treatment with heparin lowers the kidney expression of proinflammatory mediators in B/W mice but does not affect nucleosomal activation of splenocytes.

Nucleoside Derived Low Molecular Weight Gelators as a Synthetic Micro-Environment for 3D Cell Culture

2021 ◽  
Author(s):  
Omar El Hamoui ◽  
Tarek Saydé ◽  
Isabelle Svahn ◽  
Antoine Gudin ◽  
Etienne Gontier ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cell Culture ◽  
3D Cell Culture ◽  
Low Molecular Weight ◽  
Low Molecular Weight Gelators

Effects of Various Germicidal Chemicals on H.Ep. 2 Cell Culture and Herpes simplex Virus

1970 ◽  
Vol 53 (6) ◽  
pp. 1229-1236
Author(s):  
J H Blackwell ◽  
J H S Chen
Keyword(s):  
Molecular Weight ◽  
Cell Culture ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Chemical Compounds ◽  
Low Molecular Weight ◽  
Viral Inactivation ◽  
Low Molecular Weight Compounds ◽  
Simplex Virus ◽  
Ammonium Compounds

Abstract The disinfectant present in most disinfectant-virus mixtures is inherently toxic to cell cultures and thus precludes the observation of viral inactivation at low dilutions of virus. Therefore, a method employing Sephadex was devised which would remove the cytotoxic activity of virus-disinfectant mixtures. These studies show that Sephadex is an acceptable detoxicant for numerous low molecular weight compounds and does not affect the biological activity of Herpes simplex virus. Data are presented on the effect of low molecular weight chemical compounds, i.e., phenol, ethyl alcohol, quaternary ammonium compounds, and halogens, on H.Ep. 2 cell culture and the macroplaque strain of H. simplex virus.

Low molecular weight self-assembling peptide-based materials for cell culture, antimicrobial, anti-inflammatory, wound healing, anticancer, drug delivery, bioimaging and 3D bioprinting applications

Soft Matter ◽  
2020 ◽  
Vol 16 (44) ◽  
pp. 10065-10095
Author(s):  
Apurba K. Das ◽  
Pramod K. Gavel
Keyword(s):  
Drug Delivery ◽  
Molecular Weight ◽  
Cell Culture ◽  
Wound Healing ◽  
Rational Design ◽  
Low Molecular Weight ◽  
3D Bioprinting ◽  
Anticancer Drug Delivery ◽  
Self Assembling ◽  
Anti Inflammatory

This article emphasizes on the rational design and development of self-assembling peptide-based materials for cell culture, antimicrobial, anti-inflammatory, anticancer, wound healing, drug delivery, bioimaging and 3D bioprinting applications.

Tris[2-(acryloyloxy)ethyl]isocyanurate Cross-Linked Low-Molecular-Weight Polyethylenimine as Gene Delivery Carriers in Cell Culture and Dystrophic mdx Mice

2012 ◽  
Vol 23 (4) ◽  
pp. 837-845 ◽  
Author(s):  
Mingxing Wang ◽  
Jay D. Tucker ◽  
Peijuan Lu ◽  
Bo Wu ◽  
Caryn Cloer ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cell Culture ◽  
Gene Delivery ◽  
Mdx Mice ◽  
Low Molecular Weight
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