scholarly journals Comparison of microbial and transient expression (tobacco plants and plant‐cell packs) for the production and purification of the anticancer mistletoe lectin viscumin

2019 ◽  
Vol 116 (9) ◽  
pp. 2236-2249 ◽  
Author(s):  
Benjamin B. Gengenbach ◽  
Linda L. Keil ◽  
Patrick Opdensteinen ◽  
Catherine R. Müschen ◽  
Georg Melmer ◽  
...  
Keyword(s):  
Transient Expression ◽  
Plant Cell ◽  
Mistletoe Lectin ◽  
Tobacco Plants

Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression

2014 ◽  
Vol 48 (4) ◽  
pp. 208-217
Author(s):  
Y. R. Sindarovska ◽  
I. S. Golovach ◽  
V. B. Belokurova ◽  
I. M. Gerasymenko ◽  
Y. V. Sheludko ◽  
...  
Keyword(s):  
Cell Culture ◽  
Transient Expression ◽  
Plant Cell ◽  
Host Species ◽  
Plant Cell Culture ◽  
Culture Collection ◽  
Cell Culture Collection

scholarly journals OPTIMIZATION OF EXPRESSION CONDITIONS OF GENE ENCODING ANTIGEN M OF PRRSV IN LEAVES OF NICOTIANA BENTHAMIANA BY AGROINFILTRATION METHOD

2018 ◽  
Vol 16 (2) ◽  
pp. 293-300
Author(s):  
Nguyen Thi Minh Hang ◽  
Ho Thi Thuong ◽  
Nguyen Thu Giang ◽  
Pham Bich Ngoc ◽  
Nguyen Trung Nam ◽  
...  
Keyword(s):  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
M Protein ◽  
Physiological Age ◽  
Structural Protein ◽  
Gene Encoding ◽  
Tobacco Plants ◽  
Reading Frame ◽  
Prrs Virus ◽  
A Subunit

Reproductive disorders and respiratory swine (Porcine Reproductive and Respiratory Syndrome - PRRS) is an infectious disease caused by the PRRS virus (PRRSV), spread fast speeds, causing mass death when infected pigs. M protein is one of the major structural protein of the PRRSV, has a molecular weight of about 19 kDa, encoded by the open reading frame 6 (ORF6), which is used to design a subunit vaccine against back PRRSV. In this study, conditions for transient expression of the gene encoded the M protein of PRRSV in leaves of the Nicotiana benthamiana by agroinfiltration method were determined. The results show that optimal conditions for transient expression of protein M in the leaf are used the simultaneous of the A. tumefaciens strain containing vector carrying the gene encoding the protein M and A. tumefaciens containing vector carrying gene encoding supported protein HC-Pro PVY, concentration of acetosyringone with 450 µM, the bacterial cell density used to infect into leaf with OD600 is 0.5, The physiological age of the leaf suitable for infecting bacteria was young leaf and mature leaf of tobacco plants 4 to 6 weeks of age, and the transient expression time of gene encoding protein M of PRRSV in tobacco leaves is most effective 6 days after infecting. This agroinfiltration method can be used to express large amounts of protein - antigen M of PRRSV in tobacco plants to produce vaccines against this virus.

Characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene

1992 ◽  
Vol 12 (8) ◽  
pp. 3399-3406
Author(s):  
Y Wang ◽  
W Zhang ◽  
J Cao ◽  
D McElroy ◽  
R Wu
Keyword(s):  
Transient Expression ◽  
Plant Cell ◽  
Transcription Initiation ◽  
Regulatory Elements ◽  
Initiation Site ◽  
Gus Expression ◽  
Negative Regulator ◽  
Cis Acting ◽  
Highly Active ◽  
Constitutively Active

The promoter of the constitutively expressed rice (Oryza sativa) actin 1 gene (Act1) is highly active in transformed rice plants (W. Zhang, D. McElroy, and R. Wu, Plant Cell 3:1150-1160, 1991). A region 834 bp upstream of the Act1 transcription initiation site contains all the regulatory elements necessary for maximal gene expression in transformed rice protoplasts (D. McElroy, W. Zhang, J. Cao, and R. Wu, Plant Cell 2:163-171, 1990). We have constructed a series of Act1 promoter deletions fused to a bacterial beta-glucuronidase reporter sequence (Gus). Transient expression assays in transformed rice protoplasts, as well as transformed maize cells and tissues, identified two distinct cis-acting regulatory elements in the Act1 promoter. A 38-bp poly(dA-dT) region was found to be a positive regulator of Act1 promoter activity. Deletion of the poly(dA-dT) element lowered Gus expression by at least threefold compared with expression produced by the full-length Act1 promoter. By gel retardation and footprinting, we identified a ubiquitous rice protein which specifically recognizes this poly(dA-dT) element in the constitutively active Act1 promoter. A CCCAA pentamer repeat-containing region was found to be a negative regulator of the Act1 promoter in transformed rice protoplasts. Transient expression assays in different maize cells and tissues with use of the Act1 deletion constructs suggested that the CCCAA pentamer repeat region functions in a complex tissue-specific manner. A CCCAA-binding protein was detected only in root extracts.

Agrobacterium tumefaciensmediated transient expression of plant cell wall-degrading enzymes in detached sunflower leaves

2014 ◽  
Vol 30 (4) ◽  
pp. 905-915 ◽  
Author(s):  
Sang-Kyu Jung ◽  
Benjamin E. Lindenmuth ◽  
Karen A. McDonald ◽  
Min Sook Hwang ◽  
Mai Q. Nguyen Bui ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transient Expression ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Degrading Enzymes ◽  
Degrading Enzymes

scholarly journals Analysis of a maize alpha-tubulin gene promoter by transient expression and in transgenic tobacco plants

1993 ◽  
Vol 4 (6) ◽  
pp. 1043-1050 ◽  
Author(s):  
Joan Rigau ◽  
Montserrat Capellades ◽  
Lluis Montoliu ◽  
Miguel Angel Torres ◽  
Carme Romera ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Transient Expression ◽  
Gene Promoter ◽  
Transgenic Tobacco Plants ◽  
Tubulin Gene ◽  
Tobacco Plants ◽  
Alpha Tubulin

Transient Expression of Exogenous Gene into Plant Cell Mediated by PEI Nanovector

2011 ◽  
Vol 10 (6) ◽  
pp. 820-826 ◽  
Author(s):  
Ying LI ◽  
Hai-xin CUI ◽  
Yu SONG ◽  
Yao LI ◽  
Jin-li HUANG
Keyword(s):  
Transient Expression ◽  
Plant Cell ◽  
Exogenous Gene

scholarly journals Characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene.

1992 ◽  
Vol 12 (8) ◽  
pp. 3399-3406 ◽  
Author(s):  
Y Wang ◽  
W Zhang ◽  
J Cao ◽  
D McElroy ◽  
R Wu
Keyword(s):  
Transient Expression ◽  
Plant Cell ◽  
Transcription Initiation ◽  
Regulatory Elements ◽  
Initiation Site ◽  
Gus Expression ◽  
Negative Regulator ◽  
Cis Acting ◽  
Highly Active ◽  
Constitutively Active

The promoter of the constitutively expressed rice (Oryza sativa) actin 1 gene (Act1) is highly active in transformed rice plants (W. Zhang, D. McElroy, and R. Wu, Plant Cell 3:1150-1160, 1991). A region 834 bp upstream of the Act1 transcription initiation site contains all the regulatory elements necessary for maximal gene expression in transformed rice protoplasts (D. McElroy, W. Zhang, J. Cao, and R. Wu, Plant Cell 2:163-171, 1990). We have constructed a series of Act1 promoter deletions fused to a bacterial beta-glucuronidase reporter sequence (Gus). Transient expression assays in transformed rice protoplasts, as well as transformed maize cells and tissues, identified two distinct cis-acting regulatory elements in the Act1 promoter. A 38-bp poly(dA-dT) region was found to be a positive regulator of Act1 promoter activity. Deletion of the poly(dA-dT) element lowered Gus expression by at least threefold compared with expression produced by the full-length Act1 promoter. By gel retardation and footprinting, we identified a ubiquitous rice protein which specifically recognizes this poly(dA-dT) element in the constitutively active Act1 promoter. A CCCAA pentamer repeat-containing region was found to be a negative regulator of the Act1 promoter in transformed rice protoplasts. Transient expression assays in different maize cells and tissues with use of the Act1 deletion constructs suggested that the CCCAA pentamer repeat region functions in a complex tissue-specific manner. A CCCAA-binding protein was detected only in root extracts.

scholarly journals Biochemical composition of haemagglutinin-based influenza virus-like particle vaccine produced by transient expression in tobacco plants

2014 ◽  
Vol 13 (5) ◽  
pp. 717-725 ◽  
Author(s):  
François Le Mauff ◽  
Geneviève Mercier ◽  
Philippe Chan ◽  
Carole Burel ◽  
David Vaudry ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Transient Expression ◽  
Biochemical Composition ◽  
Tobacco Plants ◽  
Virus Like Particle
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