Impact of depth filtration on disulfide bond reduction during downstream processing of monoclonal antibodies from CHO cell cultures

2019 ◽  
Vol 116 (7) ◽  
pp. 1669-1683 ◽  
Author(s):  
Brian O’Mara ◽  
Zhong‐Hua Gao ◽  
Manju Kuruganti ◽  
Robert Mallett ◽  
Gautam Nayar ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Cultures ◽  
Disulfide Bond ◽  
Downstream Processing ◽  
Cho Cell ◽  
Depth Filtration ◽  
Disulfide Bond Reduction

Air sparging for prevention of antibody disulfide bond reduction in harvested CHO cell culture fluid

2014 ◽  
Vol 112 (4) ◽  
pp. 734-742 ◽  
Author(s):  
Melissa Mun ◽  
Stefanie Khoo ◽  
Aline Do Minh ◽  
James Dvornicky ◽  
Melody Trexler-Schmidt ◽  
...  
Keyword(s):  
Cell Culture ◽  
Disulfide Bond ◽  
Culture Fluid ◽  
Air Sparging ◽  
Cho Cell ◽  
Cell Culture Fluid ◽  
Cho Cell Culture ◽  
Disulfide Bond Reduction

scholarly journals Introduction and clearance of beta‐glucan in the downstream processing of monoclonal antibodies

2021 ◽  
Author(s):  
Simon Kluters ◽  
Karin Steinhauser ◽  
Roland Pfänder ◽  
Joey Studts
Keyword(s):  
Monoclonal Antibodies ◽  
Downstream Processing ◽  
Beta Glucan

Monitoring CHO cell cultures: Cell stress and early apoptosis assessment by mass spectrometry

2013 ◽  
Vol 168 (4) ◽  
pp. 452-461 ◽  
Author(s):  
Sebastian Schwamb ◽  
Bogdan Munteanu ◽  
Björn Meyer ◽  
Carsten Hopf ◽  
Mathias Hafner ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Cultures ◽  
Cell Stress ◽  
Cho Cell ◽  
Early Apoptosis

A Comparative Transcriptomics Workflow for Analyzing Microarray Data From CHO Cell Cultures

2017 ◽  
Vol 13 (3) ◽  
pp. 1700228 ◽  
Author(s):  
Chun Chen ◽  
Huong Le ◽  
Brian Follstad ◽  
Chetan T. Goudar
Keyword(s):  
Cell Cultures ◽  
Microarray Data ◽  
Comparative Transcriptomics ◽  
Cho Cell

scholarly journals Quantitative Risk assessment of product disulfide bond reduction in a recombinant protein manufacturing

2021 ◽  
Author(s):  
Yi Qing cui ◽  
rujie meng ◽  
yutong Li
Keyword(s):  
Risk Assessment ◽  
Cell Culture ◽  
Recombinant Protein ◽  
Disulfide Bond ◽  
Culture Fluid ◽  
Culture Conditions ◽  
Quantitative Risk Assessment ◽  
Prevention Measures ◽  
Recombinant Molecule ◽  
Disulfide Bond Reduction

Disulfide bond reduction occurred now and then during a recombinant protein manufacturing. Their reduction often led in the loss of batches. Various prevention measures were developed. Their implementation in a manufacturing inevitably increases its operation complexity and even might impact its product qualities and process performances. It is highly desirable to apply a reduction measure only if necessary and to apply no strong measure if a moderate one works. The reduction occurrence is determined both by the reduction sensitivity of a recombinant molecule and by the reduction power of harvested cell culture fluids. The reduction power of harvested cell culture fluids varies largely and depends on cells, lysis level, growth stage, and culture conditions. The method of the quantitative risk assessment of disulfide bond reduction was reported here. The quantitative assessment is realized by comparing the reduction sensitivity of a recombinant molecule with the reduction power of its harvested cell culture fluid.

scholarly journals Sustainable strategies based on glycine–betaine analogue ionic liquids for the recovery of monoclonal antibodies from cell culture supernatants

2019 ◽  
Vol 21 (20) ◽  
pp. 5671-5682 ◽  
Author(s):  
Emanuel V. Capela ◽  
Alexandre E. Santiago ◽  
Ana F. C. S. Rufino ◽  
Ana P. M. Tavares ◽  
Matheus M. Pereira ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Downstream Processing ◽  
Aqueous Biphasic Systems ◽  
Phase Partitioning ◽  
Hybrid Processes ◽  
Three Phase ◽  
Aqueous Biphasic ◽  
Biphasic Systems ◽  
Culture Supernatants

Ionic-liquid-based aqueous biphasic systems, three phase partitioning methods and hybrid processes combined with ultrafiltration are sustainable strategies for the downstream processing of monoclonal antibodies.

Sign in / Sign up

Export Citation Format

Share Document