scholarly journals Enhancing the functionality of a microscale bioreactor system as an industrial process development tool for mammalian perfusion culture

2019 ◽  
Vol 116 (6) ◽  
pp. 1315-1325 ◽  
Author(s):  
David J Sewell ◽  
Richard Turner ◽  
Ray Field ◽  
William Holmes ◽  
Rahul Pradhan ◽  
...  
Keyword(s):  
Process Development ◽  
Industrial Process ◽  
Perfusion Culture ◽  
Development Tool ◽  
Bioreactor System ◽  
Microscale Bioreactor

scholarly journals High-throughput microbioreactor provides a capable tool for early stage bioprocess development

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mathias Fink ◽  
Monika Cserjan-Puschmann ◽  
Daniela Reinisch ◽  
Gerald Striedner
Keyword(s):  
High Throughput ◽  
Process Development ◽  
Early Stage ◽  
Explanatory Power ◽  
Batch Mode ◽  
E Coli ◽  
Speed Up ◽  
Bioreactor System ◽  
Cell Densities ◽  
Microbial Systems

AbstractTremendous advancements in cell and protein engineering methodologies and bioinformatics have led to a vast increase in bacterial production clones and recombinant protein variants to be screened and evaluated. Consequently, an urgent need exists for efficient high-throughput (HTP) screening approaches to improve the efficiency in early process development as a basis to speed-up all subsequent steps in the course of process design and engineering. In this study, we selected the BioLector micro-bioreactor (µ-bioreactor) system as an HTP cultivation platform to screen E. coli expression clones producing representative protein candidates for biopharmaceutical applications. We evaluated the extent to which generated clones and condition screening results were transferable and comparable to results from fully controlled bioreactor systems operated in fed-batch mode at moderate or high cell densities. Direct comparison of 22 different production clones showed great transferability. We observed the same growth and expression characteristics, and identical clone rankings except one host-Fab-leader combination. This outcome demonstrates the explanatory power of HTP µ-bioreactor data and the suitability of this platform as a screening tool in upstream development of microbial systems. Fast, reliable, and transferable screening data significantly reduce experiments in fully controlled bioreactor systems and accelerate process development at lower cost.

Abstract 30: In vitro Fabrication of Human Cardiac Tissues With Porcine Perfusable Blood Vessels

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Akitoshi Inui ◽  
Hidekazu Sekine ◽  
Kazunori Sano ◽  
Izumi Dobashi ◽  
Azumi Yoshida ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Cardiac Tissue ◽  
Severe Heart Failure ◽  
Perfusion Culture ◽  
Cardiac Cell ◽  
Cell Sheets ◽  
Cardiac Tissues ◽  
Bioreactor System ◽  
Human Cardiac Tissue

The definitive treatment of severe heart failure is heart transplantation; however the number of heart transplantation procedures performed in Japan per year ranges from 30-40 due to donor shortage. Therefore, recently other treatments such as ventricular assist device or regenerative therapy by human cardiac tissue engineering have been developed and are considered as appropriate alternatives. We have developed an original technology, which was named cell-sheet based tissue engineering to fabricate functional three-dimensional tissue by layering cell sheets. The utilization of this technique allowed us to successfully engineer thick rat cardiac tissue with perfusable blood vessels in vitro. Here, we demonstrate a technique to engineer human cardiac tissue with perfusable blood vessels using cardiac cell sheets derived from human induced pluripotent stem cells, and porcine small intestine as a vascular bed for perfusion culture. The small intestine was harvested from with a branch of the superior mesenteric artery and vein and underwent mucosal resection after harvested tissue was cut open. To engineer cardiac tissue with perfusable blood vessels, cardiac cell sheets co-cultured with endothelial cells, were triple-layered and then was overlaid on the vascular bed in the bioreactor system. One day after perfusion culture, overlaid cardiac tissues pulsated spontaneously and were synchronized. The cardiac tissue construct was viable tissue without any observable necrosis. Furthermore we examined the possibility of transplantation of the in vitro engineered human cardiac tissue with the connectable host artery and vein. Engineered cardiac tissue was removed from the bioreactor system after 4-day perfusion, and transplanted to another pig heart. The branch of the superior mesenteric artery and vein of the graft were then reconnected to the host internal thoracic artery and vein. When the cardiac tissue reperfused, it began to beat spontaneously after a few minutes. We believe that this method is useful to fabricate functional cardiac tissue and may become an appropriate treatment for severe heart failure.

Multilayer coatings for the EUVL process development tool

2005 ◽  
Author(s):  
E. Louis ◽  
E. Zoethout ◽  
R. W. E. van de Kruijs ◽  
I. Nedelcu ◽  
A. E. Yakshin ◽  
...  
Keyword(s):  
Process Development ◽  
Multilayer Coatings ◽  
Development Tool

Innovation in process development: From catalyst to industrial process

2013 ◽  
Vol 218-219 ◽  
pp. 153-161 ◽  
Author(s):  
Jean-François Joly ◽  
Fabrice Giroudière ◽  
Fabrice Bertoncini
Keyword(s):  
Process Development ◽  
Industrial Process

Small-scale bioreactor system for process development and optimization

2001 ◽  
Vol 7 (2) ◽  
pp. 117-119 ◽  
Author(s):  
P. Girard ◽  
M. Jordan ◽  
M. Tsao ◽  
F.M. Wurm
Keyword(s):  
Process Development ◽  
Small Scale ◽  
Bioreactor System

scholarly journals PDU-Scale Experimental Results of CO2 Removal With Amp/Pz Solvent

2015 ◽  
Vol 36 (1) ◽  
pp. 39-48 ◽  
Author(s):  
Dariusz Śpiewak ◽  
Aleksander Krótki ◽  
Tomasz Spietz ◽  
Marcin Stec ◽  
Lucyna Więcław–Solny ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Laboratory Tests ◽  
Process Development ◽  
Industrial Process ◽  
Similar Process ◽  
Gas Mixture ◽  
Co2 Removal ◽  
Temperature Decrease ◽  
Development Unit ◽  
Reliable Representation

Abstract :This paper provides a discussion concerning results of CO2 removal from a gas mixture by the application of aqueous solutions of ethanoloamine (MEA) and 2-amino-2-methyl-1-propanol (AMP) promoted with piperazine (PZ). The studies were conducted using a process development unit. Research of such a scale provides far more reliable representation of the actual industrial process than modelling and laboratory tests. The studies comprised comparative analyses entailing identical energy supplied to a reboiler as well as tests conducted at similar process efficiencies for both solvents. The results thus obtained imply that using AMP/PZ enables reduction of the solvent heat duty. Moreover, while using AMP/PZ temperature decrease was also observed in the columns.

scholarly journals Parallel bioreactor system for accessible and reproducible anaerobic culture

2021 ◽  
Vol 3 (4) ◽  
Author(s):  
Taylor I. Monaghan ◽  
Joseph A. Baker ◽  
Gary K. Robinson ◽  
Mark Shepherd
Keyword(s):  
High Performance ◽  
Anaerobic Bacteria ◽  
Industrial Process ◽  
Culture Conditions ◽  
Batch Processes ◽  
Content Type ◽  
Point Of Entry ◽  
Redox Poise ◽  
Bioreactor System ◽  
Set Up

When working with anaerobic bacteria it is important to have the capability to perform parallel bioreactor growth experiments that are both controllable and reproducible, although capital and consumables costs for commercially available systems are often prohibitively high. Hence, a three-vessel parallel bioreactor system was designed and constructed that has the capabilities for batch and fed batch processes and can also be set up for continuous culture at a fraction of the cost of commercial systems. This system carries over many of the same functionalities of those systems with a higher price point of entry, including in-line monitoring of temperature, pH, and redox poise. To validate the performance of this system Clostridium saccharoperbutylacetonicum was grown under conditions that promote ABE fermentation, an established industrial process used to produce the solvents acetone, butanol and ethanol. Measurements of cell density, pH, and redox poise all confirmed reproducible culture conditions for these parallel vessels, and solvent quantitation via GCMS verified consistent metabolic activities for the separate cultures. In future, this system will be of interest to researchers that require high performance parallel fermentation platforms but where commercial systems are not accessible.

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