Modular pathway engineering of key carbon‐precursor supply‐pathways for improved N ‐acetylneuraminic acid production in Bacillus subtilis

2018 ◽  
Vol 115 (9) ◽  
pp. 2217-2231 ◽  
Author(s):  
Xiaolong Zhang ◽  
Yanfeng Liu ◽  
Long Liu ◽  
Miao Wang ◽  
Jianghua Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Acid Production ◽  
Pathway Engineering ◽  
Carbon Precursor ◽  
Acetylneuraminic Acid ◽  
Precursor Supply

Pathway Engineering of Bacillus subtilis for Enhanced N ‐Acetylneuraminic Acid Production via Whole‐Cell Biocatalysis

2019 ◽  
Vol 14 (7) ◽  
pp. 1800682 ◽  
Author(s):  
Lin Zhao ◽  
Rongzhen Tian ◽  
Qingyang Shen ◽  
Yanfeng Liu ◽  
Long Liu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Acid Production ◽  
Pathway Engineering ◽  
Whole Cell ◽  
Acetylneuraminic Acid ◽  
Whole Cell Biocatalysis

scholarly journals Modular pathway engineering of key precursor supply pathways for lacto-N-neotetraose production in Bacillus subtilis

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiaomin Dong ◽  
Nan Li ◽  
Zhenmin Liu ◽  
Xueqin Lv ◽  
Jianghua Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Human Milk ◽  
Feeding Strategy ◽  
Intestinal Bacteria ◽  
Fine Tuning ◽  
Pathway Engineering ◽  
Cell Maturation ◽  
Lactose Permease ◽  
Human Milk Oligosaccharides ◽  
Precursor Supply

Abstract Background Lacto-N-neotetraose (LNnT) is one of the important ingredients of human milk oligosaccharides, which can enhance immunity, regulate intestinal bacteria and promote cell maturation. Results In this study, the synthetic pathway of LNnT was constructed by co-expressing the lactose permease (LacY) β-1,3-N-acetylglucosaminyltransferase (LgtA) and β-1,4-galactostltransferase (LgtB) in Bacillus subtilis, resulting in an LNnT titer of 0.61 g/L. Then, by fine-tuning the expression level of LgtB, the growth inhibition was reduced and the LNnT titer was increased to 1.31 g/L. In addition, by modular pathway engineering, the positive-acting enzymes of the UDP-GlcNAc and UDP-Gal pathways were strengthened to balance the two key precursors supply, and the LNnT titer was improved to 1.95 g/L. Finally, the LNnT titer reached 4.52 g/L in a 3-L bioreactor with an optimal glucose and lactose feeding strategy. Conclusions In general, this study showed that the LNnT biosynthesis could be significantly increased by optimizing enzymes expression levels and modular pathway engineering for balancing the precursors supply in B. subtilis.

scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

Understanding in vivo carbon precursor supply for fatty acid synthesis in leaf tissue

2000 ◽  
Vol 22 (1) ◽  
pp. 39-50 ◽  
Author(s):  
Xiaoming Bao ◽  
Manfred Focke ◽  
Mike Pollard ◽  
John Ohlrogge
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Leaf Tissue ◽  
Acid Synthesis ◽  
Carbon Precursor ◽  
Precursor Supply

Pathway engineering of Escherichia coli for α‐ketoglutaric acid production

2020 ◽  
Vol 117 (9) ◽  
pp. 2791-2801 ◽  
Author(s):  
Xiulai Chen ◽  
Xiaoxiang Dong ◽  
Jia Liu ◽  
Qiuling Luo ◽  
Liming Liu
Keyword(s):  
Escherichia Coli ◽  
Acid Production ◽  
Pathway Engineering

Pathway engineering of Bacillus subtilis for microbial production of N-acetylglucosamine

2013 ◽  
Vol 19 ◽  
pp. 107-115 ◽  
Author(s):  
Yanfeng Liu ◽  
Long Liu ◽  
Hyun-dong Shin ◽  
Rachel R. Chen ◽  
Jianghua Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Microbial Production ◽  
Pathway Engineering

Optimization of Lactic Acid Production from Food Waste by the Saccharification of Bacillus subtili

2010 ◽  
Vol 113-116 ◽  
pp. 1080-1083 ◽  
Author(s):  
Ying Ying Liu ◽  
Qun Hui Wang ◽  
Li Wei Chen ◽  
Xiao Qiang Wang ◽  
Juan Wang
Keyword(s):  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Acid Concentration ◽  
Food Waste ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Total Sugar ◽  
Lactic Acid Concentration ◽  
Two Phase ◽  
Better Than

In order to reduce the costs of production and increase the lactic acid yields, this research adopts Bacillus subtilis to substitute enzymes. The method used in the study is two-phase fermentation - inoculate Bacillus subtilis to food waste to produce sugar, and then inoculate Lactobacillus to food waste to yield lactic acid. 87.22 g l–1 of total sugar can be obtained from non-autoclaved food waste in 30 h of saccharification at 40 centigrade. After two-phase fermentation, the optimal lactic acid concentration was 50.77g/L. The results indicate that two-phase fermentation is better than synchronous saccharification fermentation.

Characterization of a NovelN-Acetylneuraminate Lyase from Staphylococcus carnosus TM300 and Its Application toN-Acetylneuraminic Acid Production

2012 ◽  
Vol 60 (30) ◽  
pp. 7450-7456 ◽  
Author(s):  
María Inmaculada García García ◽  
Agustín Sola Carvajal ◽  
Francisco García Carmona ◽  
Álvaro Sánchez Ferrer
Keyword(s):  
Acid Production ◽  
Acetylneuraminic Acid ◽  
Staphylococcus Carnosus
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