scholarly journals Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations

2016 ◽  
Vol 114 (5) ◽  
pp. 1006-1015 ◽  
Author(s):  
Josephine Chiu ◽  
Kristin N. Valente ◽  
Nicholas E. Levy ◽  
Lie Min ◽  
Abraham M. Lenhoff ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Host Cell ◽  
Lipoprotein Lipase ◽  
Cell Protein ◽  
Host Cell Protein

A rapid cIEF–ESI–MS/MS method for host cell protein analysis of a recombinant human monoclonal antibody

Talanta ◽  
2012 ◽  
Vol 98 ◽  
pp. 253-256 ◽  
Author(s):  
Guijie Zhu ◽  
Liangliang Sun ◽  
Roza Wojcik ◽  
Dawn Kernaghan ◽  
James B. McGivney ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Host Cell ◽  
Human Monoclonal Antibody ◽  
Protein Analysis ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Esi Ms

scholarly journals Identification and characterization of host cell protein product-associated impurities in monoclonal antibody bioprocessing

2013 ◽  
Vol 111 (5) ◽  
pp. 904-912 ◽  
Author(s):  
Nicholas E. Levy ◽  
Kristin N. Valente ◽  
Leila H. Choe ◽  
Kelvin H. Lee ◽  
Abraham M. Lenhoff
Keyword(s):  
Monoclonal Antibody ◽  
Host Cell ◽  
Protein Product ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Identification And Characterization

scholarly journals Host cell protein profiling of commercial therapeutic protein drugs as a benchmark for monoclonal antibody-based therapeutic protein development

mAbs ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 1955811
Author(s):  
Rosalynn Molden ◽  
Mengqi Hu ◽  
Sook Yen E ◽  
Diana Saggese ◽  
James Reilly ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Host Cell ◽  
Therapeutic Protein ◽  
Protein Profiling ◽  
Cell Protein ◽  
Protein Drugs ◽  
Host Cell Protein

Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification

2015 ◽  
Vol 113 (6) ◽  
pp. 1260-1272 ◽  
Author(s):  
Nicholas E. Levy ◽  
Kristin N. Valente ◽  
Kelvin H. Lee ◽  
Abraham M. Lenhoff
Keyword(s):  
Monoclonal Antibody ◽  
Host Cell ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Antibody Purification

scholarly journals The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nicholas M. Negretti ◽  
Christopher R. Gourley ◽  
Prabhat K. Talukdar ◽  
Geremy Clair ◽  
Courtney M. Klappenbach ◽  
...  
Keyword(s):  
Host Cell ◽  
Campylobacter Jejuni ◽  
Foodborne Pathogen ◽  
Small Gtpase ◽  
Host Cells ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Cell Binding ◽  
Actin Reorganization ◽  
Cell Cytosol

AbstractCampylobacter jejuni is a foodborne pathogen that binds to and invades the epithelial cells lining the human intestinal tract. Maximal invasion of host cells by C. jejuni requires cell binding as well as delivery of the Cia proteins (Campylobacter invasion antigens) to the host cell cytosol via the flagellum. Here, we show that CiaD binds to the host cell protein IQGAP1 (a Ras GTPase-activating-like protein), thus displacing RacGAP1 from the IQGAP1 complex. This, in turn, leads to the unconstrained activity of the small GTPase Rac1, which is known to have roles in actin reorganization and internalization of C. jejuni. Our results represent the identification of a host cell protein targeted by a flagellar secreted effector protein and demonstrate that C. jejuni-stimulated Rac signaling is dependent on IQGAP1.

Evaluating Immunogenicity Risk Due to Host Cell Protein Impurities in Antibody-Based Biotherapeutics

2016 ◽  
Vol 18 (6) ◽  
pp. 1439-1452 ◽  
Author(s):  
Vibha Jawa ◽  
Marisa K. Joubert ◽  
Qingchun Zhang ◽  
Meghana Deshpande ◽  
Suminda Hapuarachchi ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Protein ◽  
Host Cell Protein

scholarly journals Host cell protein platform assay development for therapeutic mAb bioprocessing using mammalian cells

2015 ◽  
Vol 9 (S9) ◽  
Author(s):  
Nadine Kochanowski ◽  
Gaetan Siriez ◽  
Larissa Mukankurayija ◽  
Aurélie Delangle ◽  
Alex Murray-Smith ◽  
...  
Keyword(s):  
Host Cell ◽  
Mammalian Cells ◽  
Assay Development ◽  
Cell Protein ◽  
Host Cell Protein
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