A carbon dioxide stripping model for mammalian cell culture in manufacturing scale bioreactors

2017 ◽  
Vol 114 (6) ◽  
pp. 1184-1194 ◽  
Author(s):  
Zizhuo Xing ◽  
Amanda M. Lewis ◽  
Michael C. Borys ◽  
Zheng Jian Li
Keyword(s):  
Carbon Dioxide ◽  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture

scholarly journals Novel Carbon Dioxide-Based Method for Accurate Determination of pH and pCO2 in Mammalian Cell Culture Processes

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 520
Author(s):  
Christian Klinger ◽  
Verena Trinkaus ◽  
Tobias Wallocha
Keyword(s):  
Carbon Dioxide ◽  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Accurate Determination ◽  
Reference Method ◽  
Dissolved Carbon ◽  
Non Invasive ◽  
Offline Measurement ◽  
Pco2 Monitoring

In mammalian cell culture, especially in pharmaceutical manufacturing, pH is a critical process parameter that has to be controlled as accurately as possible. Not only does pH directly affect cell culture performance, ensuring a comparable pH is also crucial for scaling and transfer of processes. A sample-based offline pH measurement is commonly used to ensure correct bioreactor pH probe signals after sterilization and as a detection measure for drifts of probe signals. However, the sample-based pH offline measurement does not necessarily deliver required accuracy. Offsets between bioreactor pH and sample pH heavily depend on equipment, local procedures and the offline measurement method that is used. This article adequately describes a novel, non-invasive method to determine pH and pCO2 in sterile bioreactors without the need to sample and measure offline. This method utilizes the chemical correlation between carbon dioxide in the gas phase, dissolved carbon dioxide, bicarbonate and dependent proton concentrations that directly affect the pH in carbonate buffered systems. The proposed carbon dioxide-based pH reference method thereby is able to accurately determine the true pH in the bioreactor without the need to sample. The proposed method is independent of scale and bioreactor configuration and does not depend on local procedures that may differ between sites, scales or operators. Applicability of the method for both stainless steel and single use bioreactors is shown. Furthermore, the very same principles are applicable for non-invasive, online pCO2 monitoring.

Miniature Bioreactors for Rapid Bioprocess Development of Mammalian Cell Culture

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Mohd Helmi Sani ◽  
Frank Baganz
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Development ◽  
Mammalian Cell Culture ◽  
Small Scale ◽  
Cell Culture Process ◽  
Deep Wells ◽  
Working Volume ◽  
Culture Process ◽  
And Control

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Arthur H. Intosh ◽  
K. Maramorosch ◽  
C. Rechtoris
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cell ◽  
Insect Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Insect Cell Line ◽  
Cell Culture Medium

Interaction of mammalian cell culture broth with adsorbents in expanded bed adsorption of monoclonal antibodies

1999 ◽  
Vol 34 (2) ◽  
pp. 159-165 ◽  
Author(s):  
J. Feuser ◽  
M. Halfar ◽  
D. Lütkemeyer ◽  
N. Ameskamp ◽  
M.-R. Kula ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Culture Broth ◽  
Expanded Bed Adsorption ◽  
Expanded Bed

Mammalian cell culture: engineering principles and scale-up

1983 ◽  
Vol 1 (4) ◽  
pp. 102-108 ◽  
Author(s):  
M.W. Glacken ◽  
R.J. Fleischaker ◽  
A.J. Sinskey
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Scale Up ◽  
Cell Culture Engineering

Hydrogen peroxide production is affected by oxygen levels in mammalian cell culture

2017 ◽  
Vol 493 (1) ◽  
pp. 246-251 ◽  
Author(s):  
Lucas A. Maddalena ◽  
Shehab M. Selim ◽  
Joao Fonseca ◽  
Holt Messner ◽  
Shannon McGowan ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Hydrogen Peroxide Production ◽  
Oxygen Levels

Expression of recombinant calf prochymosin in mammalian cell culture

1991 ◽  
Vol 20 (2) ◽  
pp. 131-139 ◽  
Author(s):  
Meelis Kolmer ◽  
Tõnis Örd ◽  
Ismo Ulmanen
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture
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