Improvement ofEscherichia coli microaerobic oxygen metabolism byVitreoscilla hemoglobin: New insights from NAD(P)H fluorescence and culture redox potential

1995 ◽  
Vol 47 (3) ◽  
pp. 347-354 ◽  
Author(s):  
Philip S. Tsai ◽  
Govind Rao ◽  
James E. Bailey
Keyword(s):  
Redox Potential ◽  
Oxygen Metabolism ◽  
Ofescherichia Coli ◽  
Culture Redox Potential

Relationship between culture redox potential and culture fluorescence inCorynebacterium glutamicum

1988 ◽  
Vol 2 (4) ◽  
pp. 233-236 ◽  
Author(s):  
E. L. Winter ◽  
Govind Rao ◽  
T. W. Cadman
Keyword(s):  
Redox Potential ◽  
Culture Redox Potential

scholarly journals Conditions for Vigorous Growth on Sulfide and Reactor-Scale Cultivation Protocols for the Thermophilic Green Sulfur Bacterium Chlorobium tepidum

1999 ◽  
Vol 65 (1) ◽  
pp. 301-306 ◽  
Author(s):  
Biswarup Mukhopadhyay ◽  
Eric F. Johnson ◽  
Manuel Ascano
Keyword(s):  
Redox Potential ◽  
Electron Source ◽  
Chlorobium Tepidum ◽  
Sulfide Concentration ◽  
Optimum Level ◽  
Green Sulfur Bacterium ◽  
The Family ◽  
Culture Redox Potential ◽  
Vigorous Growth ◽  
Green Sulfur

ABSTRACT We describe a reactor-scale cultivation protocol for the fastest-growing and only known thermophilic member of the family Chlorobiaceae, Chlorobium tepidum. We discovered that C. tepidum would grow with sulfide as the sole electron source at rates and with final cell yields comparable to those found with thiosulfate only if the sulfide concentration was maintained below 0.1 mM and the culture redox potential was at −300 ± 20 mV. Such was also the requirement for growth in a photobioreactor when thiosulfate (optimum level, 12 mM) was used as the preferred electron source. For cultivation of C. tepidum on a 5- to 500-ml scale, we used the system of Balch and Wolfe (Appl. Environ. Microbiol. 32:781–791, 1976) using stopper-sealed serum tubes and bottles as an alternative to the methods commonly used for the cultivation of phototrophic anaerobes and obtained consistent results.

scholarly journals Diagnostic and Prognostic Possibilities of the Redox-Potential Electrochemical Measurements in Blood Plasma

2015 ◽  
Vol 70 (6) ◽  
pp. 627-632 ◽  
Author(s):  
Valeriy Ivanovich Sergienko ◽  
Mogeli Shalvovich Khubutiya ◽  
Anatoliy Konstantinovich Evseev ◽  
Aleksey Valer'evich Pinchuk ◽  
Murad Saftarovich Novruzbekov ◽  
...  
Keyword(s):  
Redox Potential ◽  
Blood Plasma ◽  
Healthy Volunteers ◽  
Postoperative Period ◽  
Reference Electrode ◽  
Oxygen Metabolism ◽  
Redox Potentials ◽  
Operating Characteristics ◽  
Transplanted Kidney ◽  
Potential Monitoring

Aims: Determination of operating characteristics of the test based on blood plasma redox potential monitoring in patients with different pathological conditions associated with impaired oxygen metabolism during treatment in postoperative period and expanding the range of parameters of the developed method of investigation of blood plasma redox potential.Methods: It were examined healthy volunteers group as following group (n =63), groups of patients with transplanted liver (n =64), kidney (n =59), and lungs (n =7). Redox potential measurements were done by platinum electrode, reference electrode was silver-chlorine one. Potentiostate IPC-ProL was used to registrate and record a dependence redox potential via time. Time of measurement was 15 min.Results: statistically significant differencees of redox potentials ranges was found in healthy volunteers and patients with transplanted kidney and liver. Ratio of measured redox potentials coincident with the values within the confidence interval in healthy volunteers was 12% in patients with transplanted kidney and 10% in patients with transplanted liver. We observed significant differences in the nature of changes of blood plasma's redox potential values in course of monitoring of subgroups of patients with and without complications after liver transplantation. It was found that sensitivity of electrochemical method was 85%, selectivity — 69,8%, precision — 85,2%.Conclusion: we discovered value ranges of blood plasma redox potential typical for different pathological states; we detected an interaction between the effect of treatment and quantitative changes in the values of the blood plasma redox potentials; criterion for early predicition of complications in patients with transplanted liver was proposed basing on redox potential monitoring during postoperative period.

Redox potential as a controlling factor in enhancing carbon tetrachloride biodegradation

1996 ◽  
Vol 34 (10) ◽  
pp. 59-66
Author(s):  
G. Jin ◽  
A. J. Englande
Keyword(s):  
Carbon Tetrachloride ◽  
Redox Potential ◽  
Critical Role ◽  
Industrial Effluents ◽  
Cost Effective ◽  
Pseudomonas Cepacia ◽  
Degradation Rate Constant ◽  
Cost Effective Technique ◽  
Culture Redox Potential ◽  
Providencia Stuartii

Biodegradation of hazardous waste is often the most cost-effective technique suitable for purifying large quantities of polluted groundwater and industrial effluents. In an effort of optimizing environmental conditions for microorganisms to degrade carbon tetrachloride, culture redox potential (Eh) was demonstrated as having a critical role. The microorganisms tested were isolated from contaminated field sites and included Pseudomonas cepacia and Providencia stuartii. Ti(III) citrate was used as a reducing agent to poise Eh at designed values. Over 99% degradation of carbon tetrachloride was effected in 3 days at −250 mV ≤ Eh ≤ −200 mV. Lesser rates were observed at Eh ≥ 0 mV. Kinetic analysis indicated that the overall degradation rate constant increased from 2.75×10−3 h−1 to 4.75×10−2 h−1 by controlling Eh at about −200 mV compared with Eh at ≥ 0 mV. Results indicated that the implementation of critical redox potential may be effective in optimizing CT biodegradation activity.

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