Development of effective modified cellulase for cellulose hydrolysis process

1995 ◽  
Vol 45 (4) ◽  
pp. 366-373 ◽  
Author(s):  
Jin Won Park ◽  
Toshio Kajiuchi
Keyword(s):  
Cellulose Hydrolysis ◽  
Hydrolysis Process ◽  
Modified Cellulase

The influence of the cellulose hydrolysis process on the structure of cellulose nanocrystals extracted from capim mombaça ( Panicum maximum )

2015 ◽  
Vol 65 ◽  
pp. 496-505 ◽  
Author(s):  
Douglas Ferreira Martins ◽  
Alexandre Bernaldino de Souza ◽  
Mariana Alves Henrique ◽  
Hudson Alves Silvério ◽  
Wilson Pires Flauzino Neto ◽  
...  
Keyword(s):  
Cellulose Nanocrystals ◽  
Cellulose Hydrolysis ◽  
Panicum Maximum ◽  
Hydrolysis Process

scholarly journals Preparation and Characterization of Sulfonated Carbon from Candlenut Shell as Catalyst for Hydrolysis of Cogon Grass Cellulose into Glucose

2020 ◽  
Vol 32 (6) ◽  
pp. 1404-1408
Author(s):  
Taslim ◽  
Dian Halimah Batubara ◽  
Seri Maulina ◽  
Iriany ◽  
Okta Bani
Keyword(s):  
Solid Acid ◽  
Batch Reactor ◽  
Solid Acid Catalyst ◽  
Acid Catalyst ◽  
Cellulose Hydrolysis ◽  
Imperata Cylindrica ◽  
Hydrolysis Process ◽  
Catalyst Production ◽  
Hydrolysis Of

Cogon grass (Imperata cylindrica) is convertible into glucose by hydrolysis process, which usually requires a catalyst. A solid acid catalyst of sulfonated carbon was used in this work. This study aimed to observe the viability of candlenut shell as carbonaceous source in solid acid catalyst production and to characterize the sulfonated carbon. The carbonization was performed at 250-550 ºC for 4 h, while sulfonation was carried out at 100-180 ºC for 6 h. Sulfonated carbon was then characterized by H+ activity/acid density test, scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) and Fourier transform infrared (FTIR) spectroscopy. Sulfonated carbon was then tested as a heterogeneous catalyst for hydrolysis reaction. The reaction was performed in a stainless steel batch reactor at 100 ºC for 6 h. Glucose formed by hydrolysis was measured by dinitrosalicylic acid (DNS) method. Results of this study suggested that sulfonated carbon derived from candlenut shell may be used as a catalyst for cogon grass cellulose hydrolysis to produce glucose

scholarly journals BIOETHANOL PRODUCTION FROM COCONUT HUSK USING THE WET GAMMA IRADIATION METHOD

2021 ◽  
Vol 14 (2) ◽  
pp. 43
Author(s):  
Putra Oktavianto ◽  
Risdiyana Setiawan ◽  
Ilhami Ariyanti ◽  
Muhammad Fadhil Jamil
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cellulose Hydrolysis ◽  
Raw Material ◽  
Bioethanol Production ◽  
Coconut Husk ◽  
Hydrolysis Process ◽  
Hydrolysis Of Cellulose ◽  
Ethanol Ethanol ◽  
Hydrolysis Of ◽  
Low Dosage

BIOETHANOL PRODUCTION FROM COCONUT HUSK USING the WET GAMMA IRRADIATION METHOD. The use of coconut husk has only been used as a material for making handicrafts such as ropes, brooms, mats, and others or just burned. The combustion of coconut husk can cause air pollution. In fact, coconut husk can be used as a raw material for bioethanol production so that the beneficial value of coconut husk will also increase. One way of bioethanol production from coconut husk is by irradiating the coconut husk. The coconut husk irradiation technique to be carried out in this study is the wet irradiation technique. Wet irradiation is carried out to accelerate the process of bioethanol production because at the time of irradiation, cellulose has been hydrolyzed and glucose has been formed so that it is more efficient in time and use of the material so that the cellulose hydrolysis process is not necessary. The coconut husk samples were wet because they were mixed with 4% NaOH and were irradiated using a gamma irradiator from STTN-BATAN Yogyakarta with a dose of 30 kGy and 50 kGy and 0 kGy (or without irradiation). Then the sample is fermented with the fungus Saccharomyces Cerevisiae from tape yeast to form ethanol. Ethanol is purified and then analyzed for concentrations using pycnometric and refractometric methods. The result is that the highest ethanol content is without irradiation (0 kGy), this is due to the low dosage used. However, the main point in this wet method research is evidence of hydrolysis of cellulose by the formation of gluoxane after irradiated wet coconut husk, and with Fehling A and B analysis, brown deposits are seen proving that glucose has been formed.

Improvement of Cellulose Hydrolysis Process and Cost Savings

2015 ◽  
Vol 11 (8) ◽  
Author(s):  
Massimo Calabrese ◽  
Elena Spessot ◽  
Paolo Bogoni ◽  
Barbara Campisi
Keyword(s):  
Cost Savings ◽  
Cellulose Hydrolysis ◽  
Hydrolysis Process

scholarly journals High Diversity of β-Glucosidase-Producing Bacteria and Their Genes Associated with Scleractinian Corals

2021 ◽  
Vol 22 (7) ◽  
pp. 3523
Author(s):  
Hongfei Su ◽  
Zhenlun Xiao ◽  
Kefu Yu ◽  
Qi Zhang ◽  
Chunrong Lu ◽  
...  
Keyword(s):  
Food Industry ◽  
Cellulose Hydrolysis ◽  
Scleractinian Corals ◽  
Cultivable Bacteria ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Hydrolysis Process ◽  
Rate Limiting ◽  
Tolerance To Ethanol ◽  
Hydrolase Family

β-Glucosidase is a microbial cellulose multienzyme that plays an important role in the regulation of the entire cellulose hydrolysis process, which is the rate-limiting step in bacterial carbon cycling in marine environments. Despite its importance in coral reefs, the diversity of β-glucosidase-producing bacteria, their genes, and enzymatic characteristics are poorly understood. In this study, 87 β-glucosidase-producing cultivable bacteria were screened from 6 genera of corals. The isolates were assigned to 21 genera, distributed among three groups: Proteobacteria, Firmicutes, and Actinobacteria. In addition, metagenomics was used to explore the genetic diversity of bacterial β-glucosidase enzymes associated with scleractinian corals, which revealed that these enzymes mainly belong to the glycosidase hydrolase family 3 (GH3). Finally, a novel recombinant β-glucosidase, referred to as Mg9373, encompassing 670 amino acids and a molecular mass of 75.2 kDa, was classified as a member of the GH3 family and successfully expressed and characterized. Mg9373 exhibited excellent tolerance to ethanol, NaCl, and glucose. Collectively, these results suggest that the diversity of β-glucosidase-producing bacteria and genes associated with scleractinian corals is high and novel, indicating great potential for applications in the food industry and agriculture.

Enzymatic Hydrolysis of Lignocellulosic Oil Palm Empty Fruit Bunch (EFB)

2015 ◽  
Vol 1113 ◽  
pp. 305-310
Author(s):  
Qadly Ameen Pahlawi ◽  
Nazlee Faisal Ghazali ◽  
Khairilanuar Mohd Hanim ◽  
Nik Azmi Nik Mahmood
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Reaction ◽  
Cellulose Hydrolysis ◽  
Enzyme Concentration ◽  
Empty Fruit Bunch ◽  
Enzyme Adsorption ◽  
Enzyme Loading ◽  
Cellulose Conversion ◽  
Hydrolysis Process ◽  
Hydrolysis Of

A preliminary study was performed on enzymatic hydrolysis process for treating empty fruit bunch (EFB) fibre. The bioconversion of cellulose hydrolysis was carried out with soluble cellulase from Trichodermareesei as the biocatalyst. Crucial trends such as substrate and enzyme loading influencing the enzymatic reaction were also studied in order to enhance the cellulose conversion. The results indicate that as the enzyme loading was increased, the EFB conversion also increased until it reached 115.63 FPU/g of enzyme concentration, beyond this values, the reverse occurred. On the other hand, as the substrate loading was increased the conversion decreased. Inhibition of enzyme adsorption by hydrolysis products appear to be the main cause of the decreasing conversion at increasing enzyme loading and substrate loading.

Determination of Kinetic Parameters for Enzymatic Cellulose Hydrolysis Using Hybrid Differential Evolution

2012 ◽  
Vol 9 (1) ◽  
Author(s):  
Yu-Ting Chen ◽  
Feng-Sheng Wang
Keyword(s):  
Kinetic Model ◽  
Differential Evolution ◽  
Geometric Mean ◽  
Cellulose Hydrolysis ◽  
Model Parameters ◽  
Ph Effects ◽  
Dynamic Behaviors ◽  
Hydrolysis Process ◽  
Dynamical Description ◽  
Initial Starting

A kinetic model could provide a dynamical description of mechanism in that it is required for analysis, design, optimization and control. Temperature and pH could affect the cellulose activity. In this study, we have introduced the kinetic model, which includes temperature and pH effects, to describe dynamic behaviors of the enzymatic hydrolysis of cellulose to glucose. A commercial enzyme was applied to the hydrolysis process. Various batch time-series observations were collected and used to estimate the model parameters of the kinetic model. Hybrid differential evolution with a geometric mean mutation was applied to determine optimal estimates, and then such estimates were used as the initial starting for a gradient-based method to obtain the refined solution. The approach is capable of predicting the dynamic behaviors of the cellulose hydrolysis process as observed extra experimental validations. Furthermore, the time-average sensitivities were applied to evaluate accuracy and robustness of the mathematical model.

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