An on-line sampling system for fermentation monitoring Using membrane inlet mass spectrometry (MIMS): Application to phenoxyacetic acid monitoring in penicillin fermentation

1994 ◽  
Vol 44 (3) ◽  
pp. 347-353 ◽  
Author(s):  
Ken Flemming Hansen ◽  
Frants R. Lauritsen ◽  
Hans Degn
Keyword(s):  
Mass Spectrometry ◽  
Phenoxyacetic Acid ◽  
Sampling System ◽  
Membrane Inlet Mass Spectrometry ◽  
Line Sampling ◽  
On Line ◽  
Fermentation Monitoring ◽  
Penicillin Fermentation

Tandem mass spectrometry for on-line fermentation monitoring

1989 ◽  
Vol 3 (6) ◽  
pp. 361-364 ◽  
Author(s):  
Mark J. Hayward ◽  
Anita K. Lister ◽  
Tapio Kotiaho ◽  
R. Graham Cooks ◽  
Glen D. Austin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
On Line ◽  
Fermentation Monitoring

scholarly journals On-Line Monitoring of Microbial Volatile Metabolites by Proton Transfer Reaction-Mass Spectrometry

2008 ◽  
Vol 74 (7) ◽  
pp. 2179-2186 ◽  
Author(s):  
Michael Bunge ◽  
Nooshin Araghipour ◽  
Tomas Mikoviny ◽  
Jürgen Dunkl ◽  
Ralf Schnitzhofer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Proton Transfer ◽  
Transfer Reaction ◽  
Proton Transfer Reaction ◽  
Reaction Mass ◽  
Specific Marker ◽  
Sampling System ◽  
Voc Emissions ◽  
Microbial Cultures ◽  
On Line

ABSTRACT A method for analysis of volatile organic compounds (VOCs) from microbial cultures was established using proton transfer reaction-mass spectrometry (PTR-MS). A newly developed sampling system was coupled to a PTR-MS instrument to allow on-line monitoring of VOCs in the dynamic headspaces of microbial cultures. The novel PTR-MS method was evaluated for four reference organisms: Escherichia coli, Shigella flexneri, Salmonella enterica, and Candida tropicalis. Headspace VOCs in sampling bottles containing actively growing cultures and uninoculated culture medium controls were sequentially analyzed by PTR-MS. Characteristic marker ions were found for certain microbial cultures: C. tropicalis could be identified by several unique markers compared with the other three organisms, and E. coli and S. enterica were distinguishable from each other and from S. flexneri by specific marker ions, demonstrating the potential of this method to differentiate between even closely related microorganisms. Although the temporal profiles of some VOCs were similar to the growth dynamics of the microbial cultures, most VOCs showed a different temporal profile, characterized by constant or decreasing VOC levels or by single or multiple peaks over 24 h of incubation. These findings strongly indicate that the temporal evolution of VOC emissions during growth must be considered if characterization or differentiation based on microbial VOC emissions is attempted. Our study may help to establish the analysis of VOCs by on-line PTR-MS as a routine method in microbiology and as a tool for monitoring environmental and biotechnological processes.

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