On-line characterization of a hybridoma cell culture process

1994 ◽  
Vol 44 (2) ◽  
pp. 170-177 ◽  
Author(s):  
Weichang Zhou ◽  
Wei-Shou Hu
Keyword(s):  
Cell Culture ◽  
Cell Culture Process ◽  
Hybridoma Cell Culture ◽  
Culture Process ◽  
On Line

Application of Quality by Design to the characterization of the cell culture process of an Fc-Fusion protein

2012 ◽  
Vol 81 (2) ◽  
pp. 426-437 ◽  
Author(s):  
Yolande Rouiller ◽  
Thomas Solacroup ◽  
Véronique Deparis ◽  
Marco Barbafieri ◽  
Ralf Gleixner ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fusion Protein ◽  
Quality By Design ◽  
Cell Culture Process ◽  
Fc Fusion Protein ◽  
Culture Process

APPLICATION OF AMBR15 MINIBIOREACTORS IN CHARACTERIZATION OF SUSPENSION MONOCLONAL ANTIBODY CHO-PRODUCER CELL CULTURE PROCESS BASED ON QBD PRINCIPLES

2021 ◽  
Vol 1 (19) ◽  
pp. 180-181
Author(s):  
A.N. Morozov ◽  
I.R. Yakhin
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Quality By Design ◽  
Systematic Approach ◽  
Cell Culture Process ◽  
Culture Process ◽  
Producer Cell

A systematic approach to characterizing the cell culture process of CHO-producers of monoclonal antibodies within the framework of the concept "Quality by design" (QbD) with the use of ambr15 mini-bioreactors (TAP Biosystems) is shown.

Miniature Bioreactors for Rapid Bioprocess Development of Mammalian Cell Culture

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Mohd Helmi Sani ◽  
Frank Baganz
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Development ◽  
Mammalian Cell Culture ◽  
Small Scale ◽  
Cell Culture Process ◽  
Deep Wells ◽  
Working Volume ◽  
Culture Process ◽  
And Control

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

Automated Confluence Measurement Method for Mesenchymal Stem Cell from Brightfield Microscopic Images

2021 ◽  
pp. 1-9
Author(s):  
Zenan Wang ◽  
Rucai Zhan ◽  
Ying Hu
Keyword(s):  
Cell Culture ◽  
Phase Contrast ◽  
Low Cost ◽  
Cost Effective ◽  
Cell Phenotype ◽  
Cell Culture Process ◽  
Reconstruction Method ◽  
Image Reconstruction Method ◽  
Contrast Microscope ◽  
Culture Process

Cell confluence is an important metric in cell culture, as proper timing is essential to maintain cell phenotype and culture quality. To estimate cell confluence, transparent cells are observed under a phase-contrast or differential interference contrast microscope by a biologist, whose estimations are error-prone and subjective. To overcome the necessity of using the phase-contrast microscope and reducing intra- and inter-observer errors, we have proposed an algorithm that automatically measures cell confluence by using a commonly used brightfield microscope. The proposed method consists of a transport-of-intensity equation-based brightfield microscopic image processing, an image reconstruction method, and an adaptive image segmentation method based on edge detection, entropy filtering, and range filtering. Experimental results have shown that our method has outperformed several popular algorithms, with an F-score of 0.84 ± 0.07, in images with various cell confluence values. The proposed algorithm is robust and accurate enough to perform confluence measurement with various lighting conditions under a low-cost brightfield microscope, making it simple and cost-effective to use for a fully automated cell culture process.

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