Purification of a recombinant protein produced in a baculovirus expression system by immobilized metal affinity chromatography

Min-Ying Wang; William E. Bentley; Vikram Vakharia

doi:10.1002/bit.260430502

Purification of a recombinant protein produced in a baculovirus expression system by immobilized metal affinity chromatography

Biotechnology and Bioengineering ◽

10.1002/bit.260430502 ◽

1994 ◽

Vol 43 (5) ◽

pp. 349-356 ◽

Cited By ~ 18

Author(s):

Min-Ying Wang ◽

William E. Bentley ◽

Vikram Vakharia

Keyword(s):

Recombinant Protein ◽

Affinity Chromatography ◽

Expression System ◽

Baculovirus Expression System ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity ◽

Baculovirus Expression

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Faculty Opinions recommendation of The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell-baculovirus expression system.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1004287.49205 ◽

2002 ◽

Author(s):

Jeffrey Schwartz

Keyword(s):

Recombinant Protein ◽

Dissolved Oxygen ◽

Insect Cell ◽

Expression System ◽

Baculovirus Expression System ◽

Baculovirus Expression ◽

Do Concentration

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Purification of Recombinant Protein Derived from the Baculovirus Expression System Using Glutathione Affinity Agarose

Baculovirus Expression Protocols ◽

10.1385/0-89603-272-8:337 ◽

2003 ◽

pp. 337-348

Author(s):

Eric J. Sorscher ◽

Maja A. Sommerfelt

Keyword(s):

Recombinant Protein ◽

Expression System ◽

Baculovirus Expression System ◽

Baculovirus Expression

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Identification of native Escherichia coli BL21 (DE3) proteins that bind to immobilized metal affinity chromatography under high imidazole conditions and use of 2D-DIGE to evaluate contamination pools with respect to recombinant protein expression level

Protein Expression and Purification ◽

10.1016/j.pep.2011.04.021 ◽

2011 ◽

Vol 78 (2) ◽

pp. 216-224 ◽

Cited By ~ 9

Author(s):

Patrick Bartlow ◽

Guy T. Uechi ◽

John J. Cardamone ◽

Tamanna Sultana ◽

McKinzie Fruchtl ◽

...

Keyword(s):

Escherichia Coli ◽

Recombinant Protein ◽

Protein Expression ◽

Affinity Chromatography ◽

Recombinant Protein Expression ◽

Immobilized Metal Affinity Chromatography ◽

Protein Expression Level ◽

2D Dige ◽

Escherichia Coli Bl21 ◽

Metal Affinity

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APPLICABILITY OF FED BATCH PROCESSES FOR IMPROVING RECOMBINANT PROTEIN PRODUCTION BY THE BACULOVIRUS EXPRESSION SYSTEM

Animal Cell Technology ◽

10.1016/b978-0-7506-0421-5.50072-6 ◽

1992 ◽

pp. 276-281 ◽

Cited By ~ 2

Author(s):

S. Reid ◽

V. Saxena ◽

P.F. Greenfield ◽

S.A. Weiss

Keyword(s):

Recombinant Protein ◽

Recombinant Protein Production ◽

Protein Production ◽

Expression System ◽

Baculovirus Expression System ◽

Batch Processes ◽

Fed Batch ◽

Baculovirus Expression

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Optimization of growth methods and recombinant protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression system

Biotechnology Progress ◽

10.1021/bp00019a004 ◽

1993 ◽

Vol 9 (1) ◽

pp. 25-30 ◽

Cited By ~ 85

Author(s):

T. J. Wickham ◽

G. R. Nemerow

Keyword(s):

Recombinant Protein ◽

Recombinant Protein Production ◽

Protein Production ◽

Insect Cells ◽

Expression System ◽

Baculovirus Expression System ◽

Baculovirus Expression ◽

Growth Methods

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Time reduction and process optimization of the baculovirus expression system for more efficient recombinant protein production in insect cells

Protein Expression and Purification ◽

10.1016/j.pep.2005.03.020 ◽

2005 ◽

Vol 42 (1) ◽

pp. 211-218 ◽

Cited By ~ 23

Author(s):

Björn Philipps ◽

Daniel Rotmann ◽

Micha Wicki ◽

Lorenz M. Mayr ◽

Michael Forstner

Keyword(s):

Recombinant Protein ◽

Process Optimization ◽

Recombinant Protein Production ◽

Protein Production ◽

Insect Cells ◽

Expression System ◽

Baculovirus Expression System ◽

Baculovirus Expression ◽

Time Reduction

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Effect of hyperosmolarity on recombinant protein productivity in baculovirus expression system

Journal of Biotechnology ◽

10.1016/s0168-1656(03)00034-8 ◽

2003 ◽

Vol 102 (3) ◽

pp. 291-300 ◽

Cited By ~ 10

Author(s):

A Olejnik ◽

W Grajek ◽

R Marecik

Keyword(s):

Recombinant Protein ◽

Expression System ◽

Baculovirus Expression System ◽

Baculovirus Expression ◽

Recombinant Protein Productivity ◽

Protein Productivity

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Detection of anti-topoisomerase I antibodies using a full length human topoisomerase I recombinant protein purified from a baculovirus expression system

Clinical & Experimental Immunology ◽

10.1111/j.1365-2249.1995.tb03655.x ◽

2008 ◽

Vol 100 (2) ◽

pp. 214-218 ◽

Cited By ~ 7

Author(s):

J. WHYTE ◽

W. C. EARNSHAW ◽

J. J. CHAMPOUX ◽

L. H. PARKER ◽

L. STEWART ◽

...

Keyword(s):

Recombinant Protein ◽

Topoisomerase I ◽

Expression System ◽

Baculovirus Expression System ◽

Full Length ◽

Baculovirus Expression

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The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell-baculovirus expression system

Biotechnology and Bioengineering ◽

10.1002/bit.10131 ◽

2001 ◽

Vol 77 (2) ◽

pp. 219-224 ◽

Cited By ~ 16

Author(s):

Fuming Zhang ◽

Mark A. Saarinen ◽

Laura J. Itle ◽

Sybil C. Lang ◽

David W. Murhammer ◽

...

Keyword(s):

Recombinant Protein ◽

Dissolved Oxygen ◽

Insect Cell ◽

Expression System ◽

Baculovirus Expression System ◽

Baculovirus Expression ◽

Do Concentration

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Factors Affecting Recombinant Protein Yields in Insect Cell/Baculovirus Expression System

Journal of the Faculty of Agriculture, Kyushu University ◽

10.5109/24092 ◽

1995 ◽

Vol 40 (1/2) ◽

pp. 45-51

Author(s):

Hiroshi Tarui ◽

Hiroyuki Kawaguchi ◽

Shinji Kaneko ◽

Toshio Hara

Keyword(s):

Recombinant Protein ◽

Insect Cell ◽

Expression System ◽

Baculovirus Expression System ◽

Factors Affecting ◽

Baculovirus Expression

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