Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process

1993 ◽  
Vol 41 (9) ◽  
pp. 846-853 ◽  
Author(s):  
George P. Philippidis ◽  
Tammy K. Smith ◽  
Charles E. Wyman
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fermentation Process ◽  
Simultaneous Saccharification And Fermentation ◽  
Fuel Ethanol ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of ◽  
Simultaneous Saccharification

Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

2019 ◽  
Vol 15 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Swapnil Gaikwad ◽  
Avinash P. Ingle ◽  
Silvio Silverio da Silva ◽  
Mahendra Rai
Keyword(s):  
Catalytic Activity ◽  
Enzymatic Hydrolysis ◽  
Immobilized Enzyme ◽  
Free Enzyme ◽  
Magnetic Nanomaterials ◽  
Sugar Production ◽  
Cellulase Enzyme ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

In Situ Microscopy for In-line Monitoring of the Enzymatic Hydrolysis of Cellulose

2013 ◽  
Vol 85 (17) ◽  
pp. 8121-8126 ◽  
Author(s):  
Britta Opitz ◽  
Andreas Prediger ◽  
Christian Lüder ◽  
Marrit Eckstein ◽  
Lutz Hilterhaus ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Enhanced enzymatic hydrolysis of cellulose in microgels

2015 ◽  
Vol 51 (52) ◽  
pp. 10502-10505 ◽  
Author(s):  
Aiping Chang ◽  
Qingshi Wu ◽  
Wenting Xu ◽  
Jianda Xie ◽  
Weitai Wu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

The physical trapping of cellulose in microgels leads to a low-ordered cellulose, favoring enzymatic hydrolysis of cellulose to generate glucose.

β-Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose

1977 ◽  
Vol 23 (2) ◽  
pp. 139-147 ◽  
Author(s):  
D. Sternberg ◽  
P. Vuayakumar ◽  
E. T. Reese
Keyword(s):  
Enzymatic Hydrolysis ◽  
Liquid Culture ◽  
Enzyme System ◽  
Microbial Production ◽  
Enzymatic Conversion ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Black Aspergilli ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of ◽  
Predominant Product

The enzymatic conversion of cellulose is catalyzed by a multiple enzyme system. The Trichoderma enzyme system has been studied extensively and has insufficient β-glucosidase (EC 3.2.1.21) activity for the practical saccharification of cellulose. The black aspergilli (A. niger and A. phoenicis) were superior producers of β-glucosidase and a method for production of this enzyme in liquid culture is presented. When Trichoderma cellulase preparations are supplemented with β-glucosidase from Aspergillus during practical saccharifications, glucose is the predominant product and the rate of saccharification is significantly increased. The stimulatory effect of β-glucosidase appears to be due to the removal of inhibitory levels of cellobiose.

scholarly journals Impact of phenolic compound as activators or inhibitors on the enzymatic hydrolysis of cellulose

2020 ◽  
Author(s):  
Ioanna Stamogiannou ◽  
John Van Camp ◽  
Guy Smagghe ◽  
Davy Van de Walle ◽  
Koen Dewettinck ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Phenolic Compound ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of
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