Determination of intraparticle immobilized enzyme distribution under moderate diffusion conditions

1992 ◽  
Vol 40 (6) ◽  
pp. 743-747 ◽  
Author(s):  
Md. M. Hossain ◽  
D. D. Do
Keyword(s):  
Immobilized Enzyme ◽  
Enzyme Distribution ◽  
Moderate Diffusion

Determination of Glucose, Sucrose, Lactose, and Ethanol in Foods and Beverages, Using Immobilized Enzyme Electrodes

1983 ◽  
Vol 66 (4) ◽  
pp. 981-984
Author(s):  
Marc Mason
Keyword(s):  
Hydrogen Peroxide ◽  
Immobilized Enzyme ◽  
Reference Electrode ◽  
Enzyme Electrodes ◽  
Enzyme Substrate ◽  
Platinum Anode ◽  
Cereal Samples ◽  
Sensitive Electrode ◽  
Better Than

Abstract An enzyme electrode coupling immobilized oxidase enzymes with a hydrogen peroxide-sensitive electrode is described. An enzyme or enzymes are immobilized in a thin microporous membrane which titsdirectly over a platinum anode held at +0.700 V relative to a Ag/AgCl2 reference electrode. When an enzyme substrate diffuses into the membrane, hydrogen peroxide is produced. The hydrogen peroxide is then oxidized at the platinum anode, producing anelectrical current that is directly proportional to hydrogen peroxide concentration, and hence substrate concentration. Glucose and sucrose in cereal samples, lactose in cheese, and ethanol in beer and wine were determined using enzyme electrodes. Relative precision of replicate analyses was better than ±2% and agreement with AOAC methods was good.

Sign in / Sign up

Export Citation Format

Share Document