Anomeric specificity of glucose uptake systems inLactococcus cremoris,Escherichia coli, andSaccharomyces cerevisiae: Mechanism, kinetics, and lmplications

1992 ◽  
Vol 40 (1) ◽  
pp. 137-146 ◽  
Author(s):  
Stig Benthin ◽  
Jens Nielsen ◽  
John Villadsen
Keyword(s):  
Escherichia Coli ◽  
Glucose Uptake ◽  
Anomeric Specificity

Carbon-13 NMR investigation of the anomeric specificity of CMP-N-acetylneuraminic acid synthetase from Escherichia coli

Biochemistry ◽  
1992 ◽  
Vol 31 (3) ◽  
pp. 775-780 ◽  
Author(s):  
Michael G. Ambrose ◽  
Stephen J. Freese ◽  
Mary S. Reinhold ◽  
Thomas G. Warner ◽  
Willie F. Vann
Keyword(s):  
Escherichia Coli ◽  
Acetylneuraminic Acid ◽  
Anomeric Specificity

The anomeric specificity of β-galactosidase and lac permease from Escherichia coli

1981 ◽  
Vol 59 (2) ◽  
pp. 100-105 ◽  
Author(s):  
R. E. Huber ◽  
K. L. Hurlburt ◽  
C. L. Turner
Keyword(s):  
Escherichia Coli ◽  
Glycosidic Bond ◽  
Lac Operon ◽  
Acceptor Reaction ◽  
Glucose Release ◽  
Bond Breakage ◽  
Lactose Metabolism ◽  
Anomeric Specificity

β-Galactosidase was found to act on α-lactose slightly more than twice as rapidly as on β-lactose for both the hydrolysis and transgalactosylis reactions. The effect was shown to be on the Vmax values; the Km values for the different anomeric forms were the same. The step of the reaction for which the enzyme has anomeric specificity was shown to be glycosidic bond breakage. The steps in glucose release or in the glucose acceptor reaction were not affected by anomeric composition. Neither allolactose hydrolysis nor transport of lactose into the cells by lac permease was sensitive to the anomeric composition of the substrate. The implications of these results for lac operon induction and for lactose metabolism are discussed.

scholarly journals Flow-cytometric study of vital cellular functions in Escherichia coli during solar disinfection (SODIS)

Microbiology ◽  
2006 ◽  
Vol 152 (6) ◽  
pp. 1719-1729 ◽  
Author(s):  
Michael Berney ◽  
Hans-Ulrich Weilenmann ◽  
Thomas Egli
Keyword(s):  
Escherichia Coli ◽  
Membrane Potential ◽  
Glucose Uptake ◽  
Efflux Pump ◽  
Cellular Functions ◽  
Solar Disinfection ◽  
E Coli ◽  
Pump Activity ◽  
Uva Light ◽  
Uptake Activity

The effectiveness of solar disinfection (SODIS), a low-cost household water treatment method for developing countries, was investigated with flow cytometry and viability stains for the enteric bacterium Escherichia coli. A better understanding of the process of injury or death of E. coli during SODIS could be gained by investigating six different cellular functions, namely: efflux pump activity (Syto 9 plus ethidium bromide), membrane potential [bis-(1,3-dibutylbarbituric acid)trimethine oxonol; DiBAC4(3)], membrane integrity (LIVE/DEAD BacLight), glucose uptake activity (2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose; 2-NBDG), total ATP concentration (BacTiter-Glo) and culturability (pour-plate method). These variables were measured in E. coli K-12 MG1655 cells that were exposed to either sunlight or artificial UVA light. The inactivation pattern of cellular functions was very similar for both light sources. A UVA light dose (fluence) of <500 kJ m−2 was enough to lower the proton motive force, such that efflux pump activity and ATP synthesis decreased significantly. The loss of membrane potential, glucose uptake activity and culturability of >80 % of the cells was observed at a fluence of ∼1500 kJ m−2, and the cytoplasmic membrane of bacterial cells became permeable at a fluence of >2500 kJ m−2. Culturable counts of stressed bacteria after anaerobic incubation on sodium pyruvate-supplemented tryptic soy agar closely correlated with the loss of membrane potential. The results strongly suggest that cells exposed to >1500 kJ m−2 solar UVA (corresponding to 530 W m−2 global sunlight intensity for 6 h) were no longer able to repair the damage and recover. Our study confirms the lethal effect of SODIS with cultivation-independent methods and gives a detailed picture of the ‘agony’ of E. coli when it is stressed with sunlight.

Dynamics of Glucose Uptake by Single Escherichia coli Cells

1999 ◽  
Vol 1 (4) ◽  
pp. 320-333 ◽  
Author(s):  
Arvind Natarajan ◽  
Friedrich Srienc
Keyword(s):  
Escherichia Coli ◽  
Glucose Uptake ◽  
Escherichia Coli Cells

A novel fluorescent derivative of glucose applicable to the assessment of glucose uptake activity of Escherichia coli

1996 ◽  
Vol 1289 (1) ◽  
pp. 5-9 ◽  
Author(s):  
Kazuaki Yoshioka ◽  
Hirokazu Takahashi ◽  
Tomoo Homma ◽  
Mikako Saito ◽  
Ki-Bong Oh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Glucose Uptake ◽  
Uptake Activity ◽  
Fluorescent Derivative

scholarly journals Metabolic Flux Responses to Pyruvate Kinase Knockout in Escherichia coli

2002 ◽  
Vol 184 (1) ◽  
pp. 152-164 ◽  
Author(s):  
Marcel Emmerling ◽  
Michael Dauner ◽  
Aaron Ponti ◽  
Jocelyne Fiaux ◽  
Michel Hochuli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Glucose Uptake ◽  
Dilution Rate ◽  
Pyruvate Kinase ◽  
Carbon Flux ◽  
Metabolic Flux ◽  
Tricarboxylic Acid Cycle ◽  
Flux Analysis ◽  
E Coli ◽  
General Response

ABSTRACT The intracellular carbon flux distribution in wild-type and pyruvate kinase-deficient Escherichia coli was estimated using biosynthetically directed fractional 13C labeling experiments with [U-13C6]glucose in glucose- or ammonia-limited chemostats, two-dimensional nuclear magnetic resonance (NMR) spectroscopy of cellular amino acids, and a comprehensive isotopomer model. The general response to disruption of both pyruvate kinase isoenzymes in E. coli was a local flux rerouting via the combined reactions of phosphoenolpyruvate (PEP) carboxylase and malic enzyme. Responses in the pentose phosphate pathway and the tricarboxylic acid cycle were strongly dependent on the environmental conditions. In addition, high futile cycling activity via the gluconeogenic PEP carboxykinase was identified at a low dilution rate in glucose-limited chemostat culture of pyruvate kinase-deficient E. coli, with a turnover that is comparable to the specific glucose uptake rate. Furthermore, flux analysis in mutant cultures indicates that glucose uptake in E. coli is not catalyzed exclusively by the phosphotransferase system in glucose-limited cultures at a low dilution rate. Reliability of the flux estimates thus obtained was verified by statistical error analysis and by comparison to intracellular carbon flux ratios that were independently calculated from the same NMR data by metabolic flux ratio analysis.

Large-scale expression, purification, and glucose uptake activity of recombinant human FGF21 in Escherichia coli

2011 ◽  
Vol 93 (2) ◽  
pp. 613-621 ◽  
Author(s):  
Minjing Zhang ◽  
Xuebin Jiang ◽  
Zhijian Su ◽  
Jiancong Lin ◽  
Qi Xiang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Glucose Uptake ◽  
Large Scale ◽  
Uptake Activity ◽  
Human Fgf21
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