A kinetic analysis of hybridoma growth and metabolism in continuous suspension culture on serum-free medium

1991 ◽  
Vol 38 (7) ◽  
pp. 733-741 ◽  
Author(s):  
G. W. Hiller ◽  
A. D. Aeschlimann ◽  
D. S. Clark ◽  
H. W. Blanch
Keyword(s):  
Suspension Culture ◽  
Kinetic Analysis ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Hybridoma Growth

HeLa-S3 Cell Growth Conditions in Serum-Free Medium and Adaptability for Proliferation in Suspension Culture

2011 ◽  
Vol 11 (2) ◽  
pp. 124-134 ◽  
Author(s):  
Sherif H. Abdeen ◽  
Ahmed M. Abdeen ◽  
Hesham A. El-Ensh ◽  
Abdalla A. El Sher
Keyword(s):  
Cell Growth ◽  
Suspension Culture ◽  
Growth Conditions ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Hela S3

HEK 293 cell suspension culture using fibronectin-adsorbed polymer nanospheres in serum-free medium

2004 ◽  
Vol 71A (1) ◽  
pp. 128-133 ◽  
Author(s):  
Ju Hee Ryu ◽  
Sang-Soo Kim ◽  
Seung-Woo Cho ◽  
Cha Yong Choi ◽  
Byung-Soo Kim
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Serum Free Medium ◽  
Free Medium ◽  
Hek 293 ◽  
Serum Free ◽  
Adsorbed Polymer ◽  
Polymer Nanospheres

Accelerate adherent animal cell growing in serum-free medium suspension culture

2019 ◽  
Vol 305 ◽  
pp. S23
Author(s):  
A.A. Elshereef ◽  
A.A. Elshereef ◽  
H. El Enshasy ◽  
S. Abdeen ◽  
T. Scheper
Keyword(s):  
Suspension Culture ◽  
Animal Cell ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Exogeneous factors are not necessary in attachment, spreading and polarization of hela cells

1978 ◽  
Vol 36 (2) ◽  
pp. 310-311
Author(s):  
W. Liebrich
Keyword(s):  
Hela Cells ◽  
Calf Serum ◽  
Glass Tube ◽  
Serum Free Medium ◽  
Nacl Solution ◽  
Free Medium ◽  
Minimum Essential Medium ◽  
Serum Free ◽  
All Solutions ◽  
Glass Support

HeLa cells were grown for 2-3 days in EAGLE'S minimum essential medium with 10% calf serum (S-MEM; Seromed, München) and then incubated for 24 hours in serum free medium (MEM). After detaching the cells with a solution of 0. 14 % EDTA and 0. 07 % trypsin (Difco, 1 : 250) they were suspended in various solutions (S-MEM = control, MEM, buffered salt solutions with or without Me++ions, 0. 9 % NaCl solution) and allowed to settle on glass tube slips (Leighton-tubes). After 5, 10, 15, 20, 25, 30, 1 45, 60 minutes 2, 3, 4, 5 hours cells were prepared for scanning electron microscopy as described by Paweletz and Schroeter. The preparations were examined in a Jeol SEM (JSM-U3) at 25 KV without tilting.The suspended spherical HeLa cells are able to adhere to the glass support in all solutions. The rate of attachment, however, is faster in solutions without serum than in the control. The latter is in agreement with the findings of other authors.

Hemoglobin Enhances the Binding of Bacterial Endotoxin to Human Endothelial Cells

1996 ◽  
Vol 76 (02) ◽  
pp. 258-262 ◽  
Author(s):  
Robert I Roth
Keyword(s):  
Endothelial Cells ◽  
Binding Protein ◽  
Bacterial Endotoxin ◽  
Dependent Manner ◽  
Serum Free Medium ◽  
Free Medium ◽  
Human Endothelial Cells ◽  
Serum Factors ◽  
Serum Free ◽  
Lps Binding

SummaryHuman endothelial cells, when incubated with bacterial endotoxin (lipopolysaccharide, LPS), modify their surface in association with prominent production of procoagulant tissue factor (TF) activity. This deleterious biological effect of LPS has been shown previously to be enhanced approximately 10-fold by the presence of hemoglobin (Hb), a recently recognized LPS binding protein that causes disaggregation of LPS and increases the biological activity of LPS in a number of in vitro assays. The present study was performed to test the hypothesis that Hb enhances the LPS-induced procoagulant activity of human umbilical vein endothelial cells (HUVEC) by increasing LPS binding to the cells. The binding of 3H-LPS to HUVEC was determined in the absence or presence of Hb or two other known LPS-binding proteins, human serum albumin (HSA) and IgG. LPS binding was substantially increased in the presence of Hb, in a Hb concentration-dependent manner, but was not increased by HSA or IgG. Hb enhancement of LPS binding was observed in serum-free medium, indicating that there was no additional requirement for any of the serum factors known to participate in the interaction of LPS with cells (e.g., lipopolysaccharide (LPS)-binding protein (LBP) and soluble CD14 (sCD14)). Hb enhancement of LPS binding also was observed in the more physiologic condition of 100% plasma. LPS-induced TF activity was stimulated by Hb, but not by HSA or IgG. In serum-free medium, TF activity was not stimulated under any of the conditions tested. Ultrafiltration of LPS was dramatically increased after incubation with Hb but not with HSA or IgG, suggesting that LPS disaggregation by Hb was responsible for the enhanced binding of LPS to HUVEC and the subsequent stimulation of TF activity.

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