Industrial scale harvest of proteins from mammalian cell culture by tangential flow filtration

1991 ◽  
Vol 38 (4) ◽  
pp. 413-422 ◽  
Author(s):  
Robert van Reis ◽  
Lee C. Leonard ◽  
Chung C. Hsu ◽  
Stuart E. Builder
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Industrial Scale ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

Investigation of single-pass tangential flow filtration (SPTFF) as an inline concentration step for cell culture harvest

2017 ◽  
Vol 524 ◽  
pp. 20-32 ◽  
Author(s):  
Abhiram Arunkumar ◽  
Nripen Singh ◽  
Michael Peck ◽  
Michael C. Borys ◽  
Zheng Jian Li
Keyword(s):  
Cell Culture ◽  
Tangential Flow Filtration ◽  
Single Pass ◽  
Tangential Flow ◽  
Concentration Step ◽  
Flow Filtration

Understanding and modeling alternating tangential flow filtration for perfusion cell culture

2014 ◽  
Vol 30 (6) ◽  
pp. 1291-1300 ◽  
Author(s):  
William Kelly ◽  
Jennifer Scully ◽  
Di Zhang ◽  
Gang Feng ◽  
Mathew Lavengood ◽  
...  
Keyword(s):  
Cell Culture ◽  
Perfusion Cell Culture ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

Miniature Bioreactors for Rapid Bioprocess Development of Mammalian Cell Culture

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Mohd Helmi Sani ◽  
Frank Baganz
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Development ◽  
Mammalian Cell Culture ◽  
Small Scale ◽  
Cell Culture Process ◽  
Deep Wells ◽  
Working Volume ◽  
Culture Process ◽  
And Control

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

Concentration of live pico- and nanoplankton by means of tangential flow filtration

1989 ◽  
Vol 11 (6) ◽  
pp. 1213-1221 ◽  
Author(s):  
Klaus-Günther Barthel ◽  
Gerald Schneider ◽  
Rolf Gradinger ◽  
Jürgen Lenz
Keyword(s):  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

High performance tangential flow filtration

1997 ◽  
Vol 56 (1) ◽  
pp. 71-82 ◽  
Author(s):  
Robert van Reis ◽  
Shishir Gadam ◽  
Leah N. Frautschy ◽  
Scott Orlando ◽  
Elizabeth M. Goodrich ◽  
...  
Keyword(s):  
High Performance ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Arthur H. Intosh ◽  
K. Maramorosch ◽  
C. Rechtoris
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cell ◽  
Insect Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Insect Cell Line ◽  
Cell Culture Medium
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