Enhanced enzymatic activity and stability of trypsin by reductive alkylation in solid phase

1990 ◽  
Vol 36 (10) ◽  
pp. 1002-1005 ◽  
Author(s):  
Ryohei F. Tsuji
Keyword(s):  
Enzymatic Activity ◽  
Solid Phase ◽  
Reductive Alkylation

The utility of reductive alkylation in solid phase peptide synthesis

Peptides ◽  
1988 ◽  
pp. 223-225 ◽  
Author(s):  
Simon J. Hocart ◽  
Ning Y. Jiang ◽  
Mary V. Nekola ◽  
David H. Coy
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Reductive Alkylation

A Reductive Alkylation Procedure Applicable to Both Solution- and Solid-Phase Syntheses of Secondary Amines

1996 ◽  
Vol 61 (19) ◽  
pp. 6720-6722 ◽  
Author(s):  
Anna K. Szardenings ◽  
Timothy S. Burkoth ◽  
Gary C. Look ◽  
David A. Campbell
Keyword(s):  
Solid Phase ◽  
Secondary Amines ◽  
Reductive Alkylation

Solid phase reductive alkylation techniques in analogue peptide bond and sidechain modification

Tetrahedron ◽  
1988 ◽  
Vol 44 (3) ◽  
pp. 835-841 ◽  
Author(s):  
David H. Coy ◽  
Simon J. Hocart ◽  
Yusuke Sasaki
Keyword(s):  
Solid Phase ◽  
Peptide Bond ◽  
Reductive Alkylation

scholarly journals How enzymes are adsorbed on soil solid phase and factors limiting its activity: A Review

2017 ◽  
Vol 31 (2) ◽  
pp. 287-302 ◽  
Author(s):  
Rahul Datta ◽  
Swati Anand ◽  
Amitava Moulick ◽  
Divyashri Baraniya ◽  
Shamina Imran Pathan ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Solid Phase ◽  
Solid Surfaces ◽  
Chemical Degradation ◽  
Enzyme Adsorption ◽  
Factors Affecting ◽  
Soil Enzymatic Activity ◽  
Different Types ◽  
Kinetics Of ◽  
Soil Solid Phase

Abstract A majority of biochemical reactions are often catalysed by different types of enzymes. Adsorption of the enzyme is an imperative phenomenon, which protects it from physical or chemical degradation resulting in enzyme reserve in soil. This article summarizes some of the key results from previous studies and provides information about how enzymes are adsorbed on the surface of the soil solid phase and how different factors affect enzymatic activity in soil. Many studies have been done separately on the soil enzymatic activity and adsorption of enzymes on solid surfaces. However, only a few studies discuss enzyme adsorption on soil perspective; hence, we attempted to facilitate the process of enzyme adsorption specifically on soil surfaces. This review is remarkably unmatched, as we have thoroughly reviewed the relevant publications related to protein adsorption and enzymatic activity. Also, the article focuses on two important aspects, adsorption of enzymes and factors limiting the activity of adsorbed enzyme, together in one paper. The first part of this review comprehensively lays emphasis on different interactions between enzymes and the soil solid phase and the kinetics of enzyme adsorption. In the second part, we encircle various factors affecting the enzymatic activity of the adsorbed enzyme in soil.

scholarly journals WOOD-DESTROYING PROPERTIES OF FOMITOPSIS PINICOLA (SW.) P. KARST. FROM MIDDLE SIBERIA

2017 ◽  
pp. 193-199
Author(s):  
Юлия (Julija) Александровна (Aleksandrovna) Литовка (Litovka) ◽  
Игорь (Igor') Николаевич (Nikolaevich) Павлов (Pavlov) ◽  
Татьяна (Tat'jana) Васильевна (Vasil'evna) Рязанова (Rjazanova) ◽  
Алена (Alena) Владимировна (Vladimirovna) Газизулина (Gazizulina) ◽  
Нелли (Nelli) Александровна (Aleksandrovna) Чупрова (Chuprova)
Keyword(s):  
Pinus Sylvestris ◽  
Enzymatic Activity ◽  
Solid Phase ◽  
Growth Parameters ◽  
Total Content ◽  
Culture Conditions ◽  
Larix Sibirica ◽  
Cellulolytic Enzymes ◽  
Pinus Sibirica ◽  
Fomitopsis Pinicola

Presents the results of a research of wood-destroying properties of the basidiomycete Fomitopsis pinicola (Sw.) P. Karst. The strains were isolated in pure culture from fruiting bodies that grew on the Abies sibirica Ledeb, Pinus sylvestris Ledeb, Pinus sibirica Du Tour and Larix sibirica Ledeb in the forests of the Krasnoyarsk Territory (56°09'–56°22' N; 92°10'–92°58' E). The cultures screened for growth parameters and the dynamics of enzymatic activity during cultivation on cellulose-containing media. The radial growth rate of Siberian strains on the agar medium and plant substrates is 1,1–3,3 mm / day; the growth coefficient is 10–42. All studied cultures synthesize extracellular cellulolytic enzymes that hydrolyze the sodium salt of carboxymethylcellulose. The enzymatic activity is in the range 0,05–0,39 U / ml with a maximum of 7–9 days. The most active strain of Fp5-15 F. pinicola used for bioconversion of coniferous plant substrates under solid-phase culture conditions. The maximum wood-destroying activity observed on pine and larch wood – the mass loss of substrates was 16 and 20%, respectively. On Pinus sylvestris sawdust, the total content of polysaccharides decreased by 1,2 times due to the fermentolysis of the hardly hydrolyzable fraction (a decrease of 1,4 times); the content of lignin substances did not change significantly. On the sawdust of Larix sibirica, the amount of polysaccharides decreased by 1,3 times in comparison with the initial substrate; lignin – in 1,2 times.

scholarly journals BIOTECHNOLOGICAL POTENTIAL OF THE SIBERIAN STRAINS OF BASIDIOMYCETES – PRODUCERS OF LIG-NOLYTIC AND CELLULOLYTIC ENZYMES

2020 ◽  
pp. 371-383
Author(s):  
Yuliya Aleksandrovna Litovka ◽  
Igor' Nikolayevich Pavlov ◽  
Polina Vasil'yevna Makolova ◽  
Anton Alekseyevich Timofeev ◽  
Ekaterina Alekseyevna Litvinova ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Ganoderma Lucidum ◽  
Solid Phase ◽  
Growth Parameters ◽  
Rapid Test ◽  
Maximum Activity ◽  
Cellulolytic Enzymes ◽  
Fomitopsis Pinicola ◽  
Xylotrophic Basidiomycetes

The results of a study of the wood-destroying properties of Siberian strains of xylotrophic basidiomycetes (Armillaria, Ganoderma, Fomitopsis, Heterobasidion and Porodaedalea) are presented. The growth parameters and enzymatic activity of the strains were determined during solid-phase and deep cultivation. Fomitopsis pinicola and Ganoderma lucidum are fast-growing fungi on cellulose-, tannin-containing nutrient media, coniferous and deciduous plant substrates (source and hydrodynamically activated). The growth coefficient on media with tannin and Na-carboxymethyl cellulose is more than 45; on plant substrates - more than 30. The strains exhibit a different growth reaction to the preliminary activation of birch sawdust (growth acceleration / growth slowdown / indifference). The maximum wood-destroying activity on wood of A. sibirica noted for the fungi A. borealis, G. tsugae, G. lucidum, F. pinicola, and P. niemelaei. The decrease in substrate mass was 8–11%; the amount of polysaccharides decreased on average 1.4 times mainly due to the fermentolysis of hard-hydrolyzable polysaccharides. High enzymatic activity of fungi observed during solid-phase and deep cultivation with an inducer. The maximum activity of phenol oxidase is characteristic of G. tsugae (1.21 units/g·s); carboxymethyl cellulase – for F.pinicola and G. lucidum strains (11.8 and 10.3 units/ml, respectively); xylanases – for H. abietinum (3.8 u/ml). The maximum accumulation of extracellular protein observed in F. pinicola (0.89 mg/ml). According to the totality of rapid test indicators, quantitative determination of enzymatic activity, growth parameters on lignocellulosic substrates and the degree of wood bioconversion, the most promising producer of lignolytic enzymes in vitro is the Gl4-16A Ganoderma lucidum strain; cellulolytic enzymes – strain Fp6-17 Fomitopsis pinicola.

Incorporation of 2-hydroxy-4-methoxybenzyl protection during peptide synthesis via reductive alkylation on the solid phase

1996 ◽  
Vol 37 (50) ◽  
pp. 9097-9100 ◽  
Author(s):  
Nicholas J. Ede ◽  
Kiah How Ang ◽  
Ian W. James ◽  
Andrew M. Bray
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Reductive Alkylation
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